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ORIGINAL RESEARCH article

Front. Oncol.
Sec. Thoracic Oncology
Volume 14 - 2024 | doi: 10.3389/fonc.2024.1462519

The clinical relevance of surgical specimens for RNA sequencing in lung cancer: A cohort study

Provisionally accepted
Jung Seop Eom Jung Seop Eom 1,2,3Soo H. Kim Soo H. Kim 2,3Kyungbin Kim Kyungbin Kim 2Ahrong Kim Ahrong Kim 1,2Hyo Y. Ahn Hyo Y. Ahn 1,2Jeongha Mok Jeongha Mok 1,2,3Jeong Su Cho Jeong Su Cho 1,2Min Ki Lee Min Ki Lee 1,2Ju S. Song Ju S. Song 4Mihyun Kim Mihyun Kim 1,2,3*
  • 1 School of Medicine, Pusan National University, Busan, Republic of Korea
  • 2 Pusan National University Hospital, Seo-gu, Republic of Korea
  • 3 Biomedical Research Institute, Pusan National University Hospital, Busan, South Sudan
  • 4 GC Genome Corporation, Yongin, Republic of Korea

The final, formatted version of the article will be published soon.

    Background Molecular screening using next-generation sequencing (NGS) in the pathologic evaluation of lung cancer is considered the standard in clinical practice; hence, we evaluated the diagnostic yields of various sampling methods for NGS.Methods NGS data from patients with lung cancer at the Pusan National University Hospital (Busan, South Korea), admitted October, 2020-April, 2023, was obtained. The sampling methods by which NGS data was obtained were divided into surgical and nonsurgical. Surgical methods included thoracoscopic surgery, surgical biopsy from the metastatic site, and lymph node excisional biopsy, whereas nonsurgical methods included bronchoscopy procedures and medical thoracoscopic biopsy.In total, we obtained 319 patients' NGS data:150 (47.0%) and 169 (53.0%) was obtained using surgical and nonsurgical methods, respectively. The overall diagnostic yield of NGS analysis was 97.5% for all samples. There were no significant differences in the success rates of deoxyribonucleic acid sequencing between surgical and nonsurgical sampling methods (98.0% vs. 96.4%, p = 0.313). On the other hand, the success rate of ribonucleic acid (RNA) sequencing was significantly lower in the surgical method group (78.0% vs. 92.3%; p < 0.001).Multivariate analysis showed that surgical sampling significantly correlated with RNA sequencing failure (Odd Ratio 4.128, 95% Confidence Interval 1.681-10.133, p = 0.002).Conclusions Small samples obtained using nonsurgical procedures are suitable for NGS analysis in clinical practice. However, surgical sampling showed a relatively lower success rate for RNA sequencing than nonsurgical sampling. This information may help in the development of protocols to reduce RNA degradation during the surgical process.

    Keywords: Next-generation sequencing, RNA, Surgical Procedures, Operative, Lung neoplasm

    Received: 10 Jul 2024; Accepted: 02 Oct 2024.

    Copyright: © 2024 Eom, Kim, Kim, Kim, Ahn, Mok, Cho, Lee, Song and Kim. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Mihyun Kim, School of Medicine, Pusan National University, Busan, 50612, Republic of Korea

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