- 1Department of General Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Shaanxi, Xi’an, China
- 2Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Shaanxi, Xi’an, China
- 3Department of Plastic, Aesthetic and Maxillofacial Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Shaanxi, Xi’an, China
Purpose: Patients with colorectal cancer (CRC) frequently present with anemia and signs of infection. However, the relationships between these factors remain unclear. This study investigated the potential association between anemia, inflammatory indices, and CRC.
Methods: We analyzed data from the 2011–2018 National Health and Nutrition Examination Survey to investigate links between anemia, inflammation, and CRC. Inflammatory indices, including the neutrophil-percentage-to-albumin ratio, neutrophil-to-lymphocyte ratio, and eosinophil-to-lymphocyte ratio, were analyzed. Following rigorous inclusion criteria, 14,114 participants were included. Statistical methods such as logistic regression and subgroup analyses were employed. Moreover, survival analysis was performed.
Results: Among the 14,114 participants, 0.6% had CRC and 11.0% were diagnosed with anemia. Anemia and inflammatory indices were associated with CRC, suggesting an increased risk (OR range: 2.03-2.50, P<0.05). Patients with CRC had lower red blood cell counts, reduced hemoglobin levels, and higher inflammatory indices. This is accompanied by an increase in the inflammatory indices, which is also a risk factor for CRC (OR range: 1.12-7.00, P<0.05). Survival analyses indicated that anemia was associated with lower survival rates, impacting all-cause, cancer, and CRC mortality.
Conclusion: Our results indicate that anemia and inflammatory indices are correlated with CRC. Patients with CRC tend to exhibit increased inflammatory indices and decreased red blood cell count and albumin levels, potentially impacting survival.
Introduction
Colorectal cancer (CRC) accounts for approximately 9.2% of all malignant neoplasms globally (1), making it the fourth deadliest cancer and the second leading cause of cancer-related mortality (2), with a survival rate of 14% (3). Age is a pivotal risk factor, linked to alterations in the intestinal microbiome and genetic predispositions (4). Although a range of determinants, including lifestyle choices and pre-existing health conditions, impact CRC prognosis, the influence of comorbidities, such as anemia, on CRC incidence and patient survival rates remains inadequately explored (5). Since anemia and inflammation are common and frequently evaluated indicators in patients with CRC, they can be practical tools for guiding clinical decisions and management strategies.
Anemia, defined as reduced hemoglobin levels or a diminished red blood cell count, affects approximately one-third of the global population (6). It is associated with increased morbidity and mortality rates, decreased productivity, and compromised neurodevelopment (7). Anemia is diagnosed when hemoglobin levels fall below 13 g/dL in males and below 12 g/dL in females (8). It frequently co-occurs with other diseases, markedly affecting survival outcomes and the prevalence of comorbidities, including infections and heart failure (9). Evidence links anemia with digestive system diseases, such as inflammatory bowel disease (10, 11) and cancer progression. Despite these associations, research on the relationship between anemia and digestive system malignancies, including CRC, is limited. Given the substantial health burden of CRC, it is imperative to explore its association with anemia (12).
Anemia is common in cancer patients because of red blood cell loss, increased destruction, and reduced functional red blood cells (13). The presence of anemia signifies a poor prognosis (14, 15) and is exacerbated by CRC treatments through surgery or chemotherapy, significantly affecting long-term outcomes (16, 17). These findings highlight the need for further research on the interplay between CRC and anemia.
A study exploring the prognostic significance of the hemoglobin, albumin, lymphocyte, and platelet (HALP) scores in solid tumors links low hemoglobin levels to adverse outcomes (18). The hemoglobin/erythrocyte distribution width ratio is a cost-effective, reproducible, and accessible prognostic marker for cancer survival (19). Additionally, in metastatic hormone-sensitive prostate cancer, low serum hemoglobin levels predict higher tumor-specific mortality, disease progression, and biochemical recurrence (20). Thus, hemoglobin levels are crucial for diagnosing anemia and assessing cancer risk.
The HALP score incorporates both anemia and inflammation markers. This study evaluated the prognostic impact of inflammatory biomarkers, specifically the neutrophil-percentage-to-albumin ratio (NPAR), neutrophil-to-lymphocyte ratio (NLR), and eosinophil-to-lymphocyte ratio (ELR), on long-term mortality in patients with CRC (6). While NPAR is a recognized inflammation indicator, few studies compare the prognostic significance of NPAR, NLR, and ELR in CRC. This study aimed to clarify these relationships (21).
We analyzed data from the National Health and Nutrition Examination Survey (NHANES) database to assess the association between inflammatory markers, hemoglobin levels, and CRC. Subgroup analysis was performed based on varying hemoglobin levels, ultimately establishing an association between anemia and inflammation on CRC. We also investigated the correlation between these factors and their impact on cancer survival.
Materials and methods
Study cohort
We utilized data from four NHANES cycles (2011–2018) to gather baseline and disease status information. Accessible on the Centers for Disease Control and Prevention (CDC) website (https://www.cdc.gov/nchs/nhanes/), the NHANES database provides extensive demographic, laboratory, and questionnaire data. This annual survey assesses the health and nutritional status of approximately 9,000 individuals in the U.S., covering demographic, economic, dietary, and health topics, along with medical, dental, physiological measurements, and laboratory tests. NHANES findings are essential for understanding disease prevalence and risk factors, facilitating health promotion and disease prevention efforts. All participants provided informed consent, and the NHANES received approval from the National Center for Health Statistics (NCHS) Research Ethics Review Board.
CRC status
We accessed cancer status data from the Medical Conditions Questionnaire (MCQ) in the “Questionnaire Data” of the NHANES database at https://wwwn.cdc.gov/Nchs/Nhanes/2011-2012/MCQ_G.htm. This section provides self-reported health condition data for both children and adults. Specifically, information on malignant tumors was obtained through two questions: MCQ220: “Have you ever been told by a doctor or other health professional that you had a tumor or a malignancy?” and MCQ230: “What type of cancer was it?” Data collection involved interviews or computer-assisted systems with rigorous quality control measures implemented by trained professionals to minimize errors and ensure data accuracy.
Diagnosis of anemia, hypertension, and diabetes
Participant data regarding anemia, hypertension, and diabetes status were obtained from various sources in the NHANES database. Laboratory data on anemia, including hemoglobin levels, were extracted from the “Standard Biochemical Profile” and the “Complete Blood Count and 5-Part Classification - Whole Blood” tests. Body measurements were collected from the “Body Measures” section. Information on hypertension and diabetes status was gathered from the “Blood Pressure & Cholesterol” and “Diabetes” questionnaire sections. Hypertension was defined as diastolic blood pressure >90 mmHg and/or systolic blood pressure >140 mmHg. Diabetes diagnosis relied on responses to the question “DIQ010: Has a doctor ever told you that you have diabetes?” Anemia was diagnosed according to the 1972 WHO standards, with thresholds of 130 g/L for men and 120 g/L for adult women (8).
Mortality data
In 2020, the NCHS released the Public Use Mortality File for NHANES, covering 1999–2018. These files provide comprehensive information on mortality status and underlying causes, identified using the International Classification of Diseases, Tenth Revision (ICD-10). Individuals lacking sufficient identifying data or deemed ineligible for public release were excluded from our study. Our analyses specifically focused on data related to all-cause and cancer-related deaths.
NPAR, NLR, and ELR
Neutrophil, lymphocyte, eosinophil, and albumin levels were obtained from basic biochemistry and complete blood counts (CBC). NPAR is calculated as the percentage of neutrophils (%) divided by serum albumin (g/dL), serving as a composite biomarker for neutrophils and albumin. NLR and ELR were calculated by dividing the absolute number of neutrophils by lymphocytes and eosinophils by lymphocytes, respectively. Additionally, the neutrophil percentage, representing the proportion of neutrophils in the white blood cell count, was determined. These biomarkers were derived from the same blood sample collected from each patient.
Covariates
This study utilized seven groups of covariate types, categorized as follows: sociodemographic characteristics, including age, race (“non-Hispanic white,” “non-Hispanic black,” “Mexican American,” “Other Hispanic,” or “other race”), sex (“male” or “female”), education (“high school or below,” “college,” or “college graduate or above”), and body mass index (BMI); and habits of daily life, including drinking (“yes,” “no,” “don’t know,” or “not recorded”) and smoking (“yes,” “no,” or “don’t know”), as well as conditions such as diabetes (“yes,” “no,” or “don’t know”), hypertension (“yes,” “no,” or “don’t know”), coronary heart disease (“yes,” “no,” or “don’t know”), and stroke (“yes,” “no,” or “don’t know”).
Statistical analysis
Statistical Product and Service Solutions (SPSS, version 26) software was used for all analyses. Continuous variables are presented as means ± standard deviations, and categorical variables as counts (percentages). Pearson’s chi-squared test was used to assess differences in categorical variables for anemia, inflammation, and cancer. T-tests were used for continuous variables. Chi-squared tests were used to investigate the associations between CRC, anemia, and inflammation, with odds ratios (ORs) and 95% confidence intervals (95% CI) calculated to quantify anemia’s risk impact. A two-tailed t-test (unpaired) or Mann-Whitney U test were used to analyze continuous variables. The χ2 test or Fisher’s exact test were used to analyze categorical variables.
We used multivariate logistic regression in SPSS to compute ORs and 95% CI, assessing the association between anemia and cancer. Seven models were calibrated to adjust for various cofounders: Model 0: no covariates were adjusted, Model 1 (age, race, sex, and education), Model 2 (smoking, drinking), Model 3 (BMI), Model 4 (diabetes), Model 5 (hypertension), Model 6 (coronary heart disease), and Model 7 (stroke). These adjustments corrected for potential confounding effects of anemia and inflammation indices on CRC. Covariates were selected based on previous studies to ensure the model validity and correction value.
We used the Kaplan–Meier method for survival analysis of patients with anemia, covering all-cause, cancer-specific, and CRC-specific mortality. Survival curves were plotted to illustrate these findings. Receiver operating characteristic (ROC) analyses were conducted to assess anemia’s predictive ability for mortality, shown as the area under the curve (AUC). Statistical significance was defined as a two-sided P value of <0.05.
Results
Study cohort selection
In this study, we analyzed data from 2011 to 2018, comprising 39,156 participants. After excluding individuals under 20 years of age, pregnant women, and those with missing data on malignant tumors, hematological and biochemical tests, BMI, chronic diseases, and smoking and drinking habits, the final analysis included 14,114 individuals, as shown in Figure 1.
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Figure 1. Flow chart of the screening process for the selection of eligible participants in NHANES 2011–2018.
Baseline characteristics of participants with CRC
Table 1 shows that 91 CRC cases accounted for 0.6% of the total cohort (14,114). The average age at CRC diagnosis was 68.84 ± 12.47 years. CRC was associated with demographic factors such as age, race, marital status, smoking, and chronic diseases. Specifically, the proportion of CRC among non-Hispanic whites was 59.34%, significantly higher than that in other ethnic groups. Smoking and chronic diseases, such as high cholesterol, hypertension, and diabetes, were also linked to CRC. The data indicated that among CRC patients, 23.08% of them experience anemia, which is significantly higher than the probability of anemia in non-CRC individuals. Inflammatory indices, including NPAR, ELR, and NLR, were elevated. However, no differences in sex, alcohol consumption, or BMI were observed between the CRC and non-CRC groups (Table 1).
Baseline characteristics of participants with anemia
Table 2 presents participant characteristics according to hemoglobin levels. Anemia definitions followed the WHO criteria: aged 15 years and older, no anemia (hemoglobin level ≥13 g/dL for males, ≥12 g/dL for females), mild anemia (11–12.9 g/dL for males, 11–11.9 g/dL for females), moderate anemia (8–10.9 g/dL for males and females), and severe anemia (<8 g/dL for males and females) (8). Our results showed that anemia affected 11.0% of the population, with a mean age of 55.44 ± 17.95 years. Anemia was more prevalent among females, older individuals, and non-Hispanic black individuals. It was also associated with chronic diseases and inflammatory indicators, but not with smoking or alcohol consumption.
Correlation analysis between CRC and complete blood count
Table 3 shows that CBC is crucial for diagnosing anemia and assessing inflammation. We classified the CBC results by CRC status and observed differences in red blood cell count (4.46 ± 0.52 & 4.69 ± 0.50 million cells/L, P<0.001) and hemoglobin level (13.45 ± 1.44 & 14.08 ± 1.52 g/dL, P<0.001) between patients with and without CRC, with corresponding differences in percentages. However, individual white blood cell count or percentages, notably neutrophils, did not show significant associations with CRC. The CBC changes observed in Table 3 are closely related to anemia and inflammation.
Association between CRC and anemia
Figure 2 shows the integration of seven covariate models into the crude model to assess anemia’s relationship with CRC. Before adjustment, the OR was 2.43 (95% CI: 1.49–3.98). After adjustment, the OR values were as follows: sex, age, race, and education [OR = 2.03 (95% CI: 1.23–3.33)], smoking and drinking [OR = 2.50 (95% CI: 1.53–4.08)], BMI [OR = 2.44 (95% CI: 1.49–3.99)], diabetes [OR = 2.23 (95% CI: 1.35–3.66)], hypertension [OR = 2.11 (95% CI: 1.29–3.47)], coronary heart disease [OR = 2.27 (95% CI: 1.38–3.72)], and stroke [OR = 2.30 (95% CI: 1.40–3.77)]. All adjusted ORs were >1, indicating an increased CRC risk with anemia in multivariate logistic regression analysis.
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Figure 2. Association between the Anemia and CRC. Crude Model, without any adjustment. Model 1: Adjusted for age, race, gender, and education. Model 2: Further adjusted for smoking and drinking habits. Model 3: Included adjustment for BMI. Model 4: Included adjustment for diabetes. Model 5: Included adjustment for hypertension. Model 6: Included adjustment for coronary heart disease. Model 7: Included adjustment for stroke.
Association between NPAR, NLR, and ELR and CRC, and their effects on the association of anemia with CRC
Table 4 shows the relationships between NPAR, NLR, ELR, and CRC. Patients with CRC generally exhibited higher inflammatory indicator values. Subsequently, after categorizing into four quartiles, NPAR was classified as follows: Q1 (<12.02), Q2 (12.02–13.66), Q3 (13.67–15.40), and Q4 (≥15.40); for NLR, Q1 (1.44), Q2 (1.44–1.93), Q3 (1.93–2.66), and Q4 (≥2.66); and for ELR, Q1 (<0.05), Q2 (0.05–0.07), Q3 (0.07–0.13), and Q4 (≥0.13). Across all quartiles, the proportion of patients with CRC was higher than those without CRC. Subgroup analysis indicated a higher proportion of patients with CRC in the Q3 and Q4 quartiles compared to Q1 and Q2, particularly with ELR exceeding 70% in Q3 and Q4.
Figure 3 shows the integration of these models to examine the relationship between inflammatory indicators (NPAR, NLR, and ELR), and CRC. Before adjustment, ORs were: NPAR [OR = 1.14 (95% CI: 1.06–1.23)], NLR [OR = 1.12 (95% CI: 1.02–1.23)], and ELR [OR = 7.00 (95% CI: 1.23–39.81)]. After adjustment most indicators suggested elevated CRC risk, suggesting the potential role of inflammatory markers in CRC development.
![www.frontiersin.org](https://www.frontiersin.org/files/Articles/1457886/fonc-14-1457886-HTML/image_m/fonc-14-1457886-g003.jpg)
Figure 3. Association between CRC and (A) NPAP, (B) NLR and (C) ELR. Crude Model, without any adjustment; Model 1: Adjusted for age, race, gender, and education. Model 2: Further adjusted for smoking and drinking habits. Model 3: Included adjustment for BMI. Model 4: Included adjustment for diabetes. Model 5: Included adjustment for hypertension. Model 6: Included adjustment for coronary heart disease. Model 7: Included adjustment for stroke.
Figure 4 shows that anemia’s impact on CRC risk varies across NPAR, NLR, and ELR subgroups. The ORs were: NPAR Q1 (OR = 3.07, 95% CI: 1.01–9.37), NPAR Q3 (OR = 3.82, 95% CI: 1.59–9.14), NLR Q2 (OR = 3.01, 95% CI: 1.12–8.11), NLR Q4 (OR = 2.39, 95% CI: 1.11–5.16), ELR Q2 (OR = 3.55, 95% CI: 0.95–13.32), and ELR Q3 (OR = 2.50, 95% CI: 1.09–5.75). These indicators suggest that anemia may increase CRC risk within the four categories of NPAR, NLR, and ELR.
Anemia and cancer mortality outcomes
In our analysis of follow-up outcomes and death data from 2011 to 2018, we aimed to construct survival curves and authenticate all-cause and cancer-specific mortality data in patients with CRC. After adjusting for potential confounders, individuals with anemia had significantly poorer survival outcomes than those without anemia across all measured endpoints. The area under the ROC curve indicated a significant difference in cancer-related mortality between those with and without anemia (Figure 5). These findings underscore the negative influence of anemia on cancer prognosis and mortality.
![www.frontiersin.org](https://www.frontiersin.org/files/Articles/1457886/fonc-14-1457886-HTML/image_m/fonc-14-1457886-g005.jpg)
Figure 5. Kaplan-Meier survival curves for mortality outcomes. All-cause and cancer mortality and ROC curves (A, B) All-cause mortality survival curve and ROC curve for all-cause mortality, (C, D) Cancer mortality survival curve and ROC curve for cancer mortality, (E, F) CRC mortality survival curve and ROC curve for CRC mortality.
Discussion
This cohort study, spanning from 2011 to 2018, utilized comprehensive questionnaire information reflecting the demographics of the general U.S. population. Our results show an association between anemia, inflammatory markers (NPAR, ELR, and NLR), and CRC. Statistical analysis indicated that anemia and higher levels of these markers increased the risk of CRC. Hematological analysis revealed notable disparities in red blood cell-related indicators, with a higher prevalence of anemia in patients with CRC, especially older individuals (22). Cancer and chemotherapy-induced anemia are common (23), with prevalence rates between 30% and 90% (23, 24). Subgroup analysis of hemoglobin levels highlighted distinct distributions in patients with CRC. Moreover, survival curve analysis revealed increased cancer mortality rates among patients with anemia, spanning multiple causes of death. Despite the clinical significance of this association, research on the relationship between CRC and anemia remains limited, impacting patient quality of life and prognosis (25). Further investigation is needed to understand this relationship and inform clinical management strategies.
Anemia is more prevalent in advanced stages of CRC (26), with survival curve analysis showing elevated cancer mortality rates among patients with anemia across various causes of death. Anemia not only diminishes the quality of life of patients with cancer but also negatively impacts cancer prognosis, potentially increasing fivefold (27).
Anemia in patients with CRC is often due to gastrointestinal bleeding or iron deficiency (28). Our study observed an increased risk of CRC associated with anemia, leading to delayed treatment and worse survival (29). After adjusting for covariates, combining analysis of anemia with NPAR, ELR, and NLR showed a predictive effect on CRC risk. Simple blood tests can determine the presence of anemia or elevated NPAR, ELR, or NLR. Further prospective studies are necessary to establish a direct relationship between these indicators and CRC. This novel finding sheds light on an underexplored aspect of the association between CRC and anemia, suggesting that this association may disrupt the intestinal microbiota and influence cancer progression (30). Further research is warranted to elucidate these relationships and their implications for clinical practice and public health interventions.
Inflammation plays a pivotal role in both CRC and CRC with anemia. Our evaluation of CRC using inflammatory markers (NPAR, NLR, and ELR) revealed that elevated markers correlated with increased risk. NPAR, a measurable inflammatory indicator (31), has a significant predictive value in the prognosis of various malignant tumors (32, 33). Similarly, NLR has been linked to systemic inflammation in critically ill patients (34) and elevated cancer risk and mortality (35, 36). Studies have demonstrated an association between NLR and cancer risk (37), along with its impact on overall survival in patients with non-small cell lung cancer (38). Elevated ELR may indicate a poor cancer prognosis (39, 40). Integrating these biochemical indicators enhances prognostic accuracy for patients with anemic CRC, aiding in better understanding and management of CRC with anemia.
In addition to anemia and inflammation indices, there are now other methods used for the diagnosis of CRC, and deep learning (DL) is one of them. DL is a subtype of artificial intelligence (AI), and convolutional neural networks (CNN) is a form of supervised DL with multiple layers of artificial neurons. Scientists have trained a CNN to identify malignant tumors and achieve accurate diagnoses (41). In this way, DL can help researchers identify CRC more efficiently and accurately (42).
It is crucial to acknowledge the limitations of our study, particularly regarding the analysis of hemoglobin levels, a critical indicator of anemia. We did not specifically investigate severe anemia (hemoglobin < 6.0 g/dL), which may be more prevalent among patients with cancer and significantly impact those with CRC. Additionally, our study’s observational nature means residual confounding factors cannot be entirely ruled out (43).
Future research should include a more comprehensive analysis of hemoglobin levels, particularly severe anemia, and further explore the mechanistic links between anemia and CRC development. Understanding how cancer, chronic infections, and inflammation contribute to anemia could lead to therapeutic interventions targeting anemia in patients with CRC, ultimately improving outcomes. Preventing and correcting anemia could potentially improve survival for individuals with CRC. Further studies and clinical trials are needed to explore strategies for managing anemia in patients with CRC to improve survival and quality of life.
Conclusion
Our study highlights the association between anemia, inflammatory indices, and CRC, suggesting that anemia and inflammation may be significant risk indicators in patients with CRC. Patients with anemia have lower survival rates, underscoring the urgent need to address anemia in CRC management. Early detection and effective management of anemia are essential for improving the prognosis and quality of life of patients with CRC. These findings emphasize the importance of continued research and clinical efforts to understand and address the complexity of anemia in CRC, ultimately improving patient care and outcomes.
Data availability statement
The original contributions presented in the study are included in the article/supplementary material. Further inquiries can be directed to the corresponding author/s.
Ethics statement
Prior to participating in NHANES, all participants provided informed consent. The NHANES dataset is publicly available through the website. The NHANES protocol was approved by the institutional review board of the Centers for Disease Control and Prevention https://www.cdc.gov/nchs/nhanes/irba98.htm.
Author contributions
CQ: Writing – original draft. SY: Methodology, Writing – review & editing. TS: Data curation, Formal analysis, Writing – review & editing. QP: Investigation, Methodology, Writing – review & editing. SX: Supervision, Validation, Writing – original draft. YL: Validation, Visualization, Writing – original draft.
Funding
The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was supported by Natural Science Basic. Research Plan in Shaanxi Province of China (No. 2024JC-YBQN-0926).
Acknowledgments
We express gratitude to the NHANES participants and staff members for their invaluable contribution to data collection and for making the data publicly available and thank all individuals who participated in this research.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Abbreviations
CRC, colorectal cancer; NHANES, National Health and Nutrition Examination Survey; HALP, hemoglobin, albumin, lymphocyte, and platelet; NPAR, neutrophil-percentage-to-albumin ratio; NLR, neutrophil-to-lymphocyte ratio; ELR, eosinophil-to-lymphocyte ratio; MCQ, Medical Conditions Questionnaire; CBC, complete blood count; BMI, body mass index; DL, deep learning; AI, artificial intelligence; CNN, convolutional neural networks.
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Keywords: colorectal cancer, NHANES, anemia, inflammation indices, risk
Citation: Qu C, Yang S, Shen T, Peng Q, Sun X and Lin Y (2024) Exploring the influence of anemia and inflammation indices on colorectal cancer: analysis of the national health and nutrition examination survey From 2011 to 2018. Front. Oncol. 14:1457886. doi: 10.3389/fonc.2024.1457886
Received: 03 July 2024; Accepted: 15 August 2024;
Published: 03 September 2024.
Edited by:
Francesk Mulita, General University Hospital of Patras, GreeceReviewed by:
Angelis Peteinaris, University of Patras, GreeceAndreas Antzoulas, General University Hospital of Patras, Greece
Copyright © 2024 Qu, Yang, Shen, Peng, Sun and Lin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Xuejun Sun, sunxy@mail.xjtu.edu.cn; Yuyao Lin, yuyaolin@xjtufh.edu.cn
†These authors have contributed equally to this work