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ORIGINAL RESEARCH article

Front. Oncol.
Sec. Cancer Genetics
Volume 14 - 2024 | doi: 10.3389/fonc.2024.1456986
This article is part of the Research Topic Exploring DNA Repair Defects and Metabolic Vulnerability to Enhance Immunotherapy response View all articles

Comprehensive analysis of splicing factor SRs-related gene characteristics: predicting osteosarcoma prognosis and immune regulation status

Provisionally accepted
Changhai Long Changhai Long 1,2Biao Ma Biao Ma 1,3Sijing Liu Sijing Liu 1,3*
  • 1 Guangdong Medical University, Zhanjiang, China
  • 2 Department of Ophthalmology, Second Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong Province, China
  • 3 Second Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong Province, China

The final, formatted version of the article will be published soon.

    Objective: To investigate the impact of SRs-associated genes on the overall survival and prognosis of osteosarcoma patients through bulk and single-cell RNA-seq transcriptome analysis.In this study, we constructed a prognosis model based on serine/argininerich splicing factors (SRSF) and predicted the survival of osteosarcoma patients. By analyzing single-cell RNA sequencing data and applying AUCell enrichment analysis, we revealed oncogenic pathways of SRSF in osteosarcoma immune cells. Additionally, we described the regulatory role of SRSF7 in pan-cancer.Results: Lasso regression analysis identified 6 key SRSF-associated genes, and a prognosis prediction model was established. The upregulation of these pathways revealed that SRSFs promote tumor cell proliferation and survival by regulating related signaling pathways and help tumor cells evade host immune surveillance. Additionally, by grouping single-cell data using AUCell, we found significant differences in T cell expression between high and low-risk groups. The analysis results indicated that the regulatory activity of SRSFs is closely related to T cell function, particularly in regulating immune responses and promoting immune evasion. Furthermore, SRSF7 regulates cell proliferation and apoptosis.SRs-associated genes play a critical regulatory role in osteosarcoma. T cells are key in regulating immune responses and promoting immune evasion through SRs genes. SRSF7 is a significant gene influencing the occurrence and development of osteosarcoma.

    Keywords: Serine/Arginine-Rich Splicing Factor (SR) related genes, Osteosarcoma, SRSF7, T cell, immune microenvironment

    Received: 29 Jun 2024; Accepted: 09 Aug 2024.

    Copyright: © 2024 Long, Ma and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Sijing Liu, Guangdong Medical University, Zhanjiang, China

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