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ORIGINAL RESEARCH article

Front. Oncol.
Sec. Cancer Genetics
Volume 14 - 2024 | doi: 10.3389/fonc.2024.1448502

Long noncoding RNA FAM87A is associated with overall survival and promotes cell migration and invasion in gastric cancer

Provisionally accepted
Xue Jiang Xue Jiang Manjiao Lu Manjiao Lu Wenna Fan Wenna Fan Jing Song Jing Song Fangzhou Song Fangzhou Song *
  • Chongqing Medical University, Chongqing, China

The final, formatted version of the article will be published soon.

    The role of long noncoding RNAs (lncRNAs) in the invasion and metastasis of gastric cancer remains largely unclear.Omnibus databases, we identified differentially expressed genes in gastric cancer. Using the Catalogue of Somatic Mutations in Cancer (COSMIC) database-curated gene set, lncRNAs associated with invasion and metastasis were identified. The Cox analyses were performed to identify prognostic lncRNAs. The competing endogenous RNA (ceRNA) regulation network was constructed to identify hub lncRNAs in gastric cancer. Functional and pathway analyses were used to investigate the function of identified lncRNAs. RT-qPCR and Transwell assays were used to investigate the expression in gastric cancer tissues and functions in gastric cancer cell lines.Results: Based on GEO and TCGA databases, 111 differentially expressed lncRNAs were identified between gastric cancer and normal samples. 43 lncRNAs were significantly correlated with hallmark genes of cancer invasion and metastasis. Among them, as a hub lncRNA in the invasion-related ceRNA regulation network, FAM87A showed potential regulation on MAPK signaling and transforming growth factor (TGF) signaling cascade, such as TGFB2, TGFBR1, and TGFBR2. Furthermore, FAM87A also showed significant correlation with cell adhesion molecules, such as Integrin alpha 6 (ITGA6) and Contactin-1 (CNTN1). RT-qPCR experiments showed that FAM87A expression was upregulated in gastric cancer tissues compared to normal samples (n=30).Transwell assays showed that FAM87A knockdown inhibited the migration and invasion abilities of gastric cancer cells in vitro. Notably, clinical data analysis showed that lncRNA FAM87A could be an independent factor for overall survival of patients with gastric cancer. Conclusions: LncRNA FAM87A may play a pivotal role in regulating migration and invasion of gastric cancer cells. FAM87A could be a potential biomarker for the overall survival of gastric cancer.

    Keywords: FAM87A, gastric cancer, overall survival, ceRNA, invasion and metastasis, Tumor Microenvironment

    Received: 13 Jun 2024; Accepted: 30 Aug 2024.

    Copyright: © 2024 Jiang, Lu, Fan, Song and Song. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Fangzhou Song, Chongqing Medical University, Chongqing, China

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