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ORIGINAL RESEARCH article

Front. Oncol.
Sec. Molecular and Cellular Oncology
Volume 14 - 2024 | doi: 10.3389/fonc.2024.1443346
This article is part of the Research Topic Deciphering the Role of Signature Genes in Cancer Prognosis and Therapy Resistance View all 6 articles

Expression of HOTAIR and PTGS2 as potential biomarkers in Chronic Myeloid Leukemia patients in Brazil

Provisionally accepted
Ana Paula Kubaski Benevides Ana Paula Kubaski Benevides 1Anelis Maria Marin Anelis Maria Marin 1Denise K. AnelisWosniaki Denise K. AnelisWosniaki 1Rafaela N. Oliveira Rafaela N. Oliveira 1Gabriela M. Koerich Gabriela M. Koerich 1Bianca N. Kusma Bianca N. Kusma 1Eduardo C. Munhoz Eduardo C. Munhoz 2Dalila L. Zanette Dalila L. Zanette 1Mateus N. Aoki Mateus N. Aoki 1*
  • 1 Carlos Chagas Institute (ICC), Oswaldo Cruz Foundation, Curitiba, Brazil
  • 2 Hospital Erasto Gaertner, Curitiba, Paraná, Brazil

The final, formatted version of the article will be published soon.

    Chronic myeloid leukemia (CML) is a clonal myeloproliferative neoplasm in which all the patients has the translocation (9;22) that generates de BCR::ABL1 tyrosine kinase. Despite this disease possessing a good biomarker (BCR::ABL1 transcripts level) for diagnosis and prognosis, many studies has been performed to investigate other molecules, such as the long noncoding RNAs (lncRNAs) and mRNAs, as potential biomarkers with the aim of predicting a change in BCR::ABL1 levels and as an associated biomarker. A RNAseq was performed comparing 6 CML patients with high BCR::ABL1 expression with 6 healthy control individuals, comprising the investigation cohort to investigate these molecules. To validate the results obtained by RNAseq, samples of 87 CML patients and 42 healthy controls were used in the validation cohort by RT-qPCR assays. The results showed lower expression of HOTAIR and PTGS2 in CML patients. The HOTAIR expression is inversely associated with BCR::ABL1 expression in imatinib-treated CML patients, and to PTGS2 showing that CML patients with high BCR::ABL1 expression showed reduced PTGS2 expression.

    Keywords: CML, biomarkers, lncRNAs, HOTAIR, PTGS2, RNAseq

    Received: 03 Jun 2024; Accepted: 16 Sep 2024.

    Copyright: © 2024 Kubaski Benevides, Marin, AnelisWosniaki, Oliveira, Koerich, Kusma, Munhoz, Zanette and Aoki. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Mateus N. Aoki, Carlos Chagas Institute (ICC), Oswaldo Cruz Foundation, Curitiba, Brazil

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