Huonggiang Nguyen ¹ Uijin Juang ¹ Suhwan Gwon ¹ Woohyeong Jung ¹ Qingzhi Huang ¹ Soohyeon Lee ¹ Beomwoo Lee ¹ So Hee Kwon ² Jongsun Park ^1* Seon-Hwan Kim ^1*

• ¹ School of Medicine, Chungnam National University, Daejeon, Republic of Korea
• ² Yonsei University, Seoul, Seoul, Republic of Korea

The mutated in colorectal cancer (MCC) gene was initially identified as a candidate tumor suppressor gene in colorectal cancer, acting as a negative regulator of cell cycle progression.However, its functional roles in brain tumors, particularly glioblastoma, remains largely unexplored. This study reveals a significant association between MCC status and glioblastoma.In vitro analyses revealed elevated protein and mRNA levels of MCC in several glioblastoma cell lines (U118MG and T98G). Silencing MCC expression via siRNA-mediated knockdown resulted in increased proliferation and migration of these cell lines. Supporting these findings, analyses of The Cancer Genome Atlas (TCGA), Chinese Glioma Genome Atlas (CGGA), and Genotype-Tissue Expression (GTEx) databases confirmed higher MCC expression in glioblastoma tumors than in normal brain tissue. Importantly, we observed that high MCC expression was associated with poor prognosis in glioblastoma patients, highlighting its potential role in disease progression. Additionally, this study identifies a nuclear localization of MCC in the glioblastoma cell line. Collectively, these findings indicate that MCC expression is significantly upregulated in glioblastoma and may play a role in its pathophysiology, warranting further investigation.