AUTHOR=Yuan Jinrui , Wen Mengke , Matnuri Amina , Zhao Shihong , Jian Ning , Shen Guqun 

TITLE=The expression of lnc-CCDC170-4:1, ESR1, lncRNA SRA, and CYP19A1 in cervical squamous cell carcinoma and their relationship with the clinical characteristics

JOURNAL=Frontiers in Oncology

VOLUME=14

YEAR=2024

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2024.1430826

DOI=10.3389/fonc.2024.1430826

ISSN=2234-943X

ABSTRACT=<sec><title>Introduction</title><p>The occurrence of cervical cancer may be related to estrogen and estrogen receptors. This study investigated the expression of lnc-CCDC170–4:1, <italic>ESR1</italic> (estrogen receptor 1), lncRNA SRA, and <italic>CYP19A1</italic> (aromatase) in cervical squamous cell carcinoma tissues, as well as their relationship with the clinical characteristics of patients.</p></sec><sec><title>Methods</title><p>Whole transcriptome sequencing analysis was performed on cervical squamous cell carcinoma tissues (n=4) and normal tissues (n=4). The expressions of lnc-CCDC170–4:1, <italic>ESR1</italic>, lncRNA SRA, and <italic>CYP19A1</italic> were validated in 26 cases of cervical cancer tissue and 30 cases of normal cervical tissue using qRT-PCR. The relationship of gene expression with the clinical characteristics and 5-year overall survival rates of cervical cancer patients was analyzed.</p></sec><sec><title>Results</title><p>The expression levels of CYP19A1 and lncRNA SRA were upregulated, while those of ESR1 and lnc-CCDC170–4:1 were downregulated in cervical squamous cell carcinoma tissue. However, their expression was not related to 5-year overall survival rates (p&gt;0.05). Low expression of lnc-CCDC170–4:1 was associated with lymph node metastasis (p=0.030) and Tumor size (p=0.047), Low expression of ESR was associated with FIGO Staging (p=0.041)and Tumor size(p=0.002),High expression of LncSRA was associated with FIGO Staging(p=0.004).</p></sec><sec><title>Conclusion</title><p>Estrogen and estrogen receptors may play a role in the occurrence and development of cervical squamous cell carcinoma. Low expression of lnc-CCDC170–4:1 and ESR1 are associated with lymph node metastasis and FIGO stage, so it may be a potential biomarker to evaluate the prognosis of cervical cancer.</p></sec>