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CLINICAL TRIAL article

Front. Oncol.
Sec. Surgical Oncology
Volume 14 - 2024 | doi: 10.3389/fonc.2024.1338811

The Use of Matrine To Inhibit Osteosarcoma Cell Proliferation via the Regulation of the MAPK/ERK Signaling Pathway

Provisionally accepted
Xincheng Huang Xincheng Huang 1Jun Zeng Jun Zeng 2*Siyuan Ruan Siyuan Ruan 1*Zhuolin Lei Zhuolin Lei 1*Jingyuan Zhang Jingyuan Zhang 1Hong Cao Hong Cao 1*
  • 1 Hubei University of Medicine, Shiyan, China
  • 2 Department of Anesthesiology, Shiyan People's Hospital, Shiyan, China

The final, formatted version of the article will be published soon.

    Background: Matrine is an alkaloid extracted from Sophorus beans of the legume family, and it has significant effects and a variety of pharmacological activities. Osteosarcoma(OS) is a common malignant bone tumor that is characterized by high incidence and rapid progression.There have been some preliminary studies on the therapeutic effect of matrine on OS, but the specific mechanism remains unclear.The aim of this study was to investigate the antitumor effect of matrine on HOS cells and the underlying molecular mechanism.The effects of matrine on the proliferation, apoptosis and cell cycle progression of HOS cells were determined by CCK-8 assay, TUNEL assay and flow cytometry in vitro. Wound healing and Transwell invasion assays were used to observe the effect of matrine on the migration and invasion of HOS cells. The mechanism underlying the antitumor effect of matrine on HOS cells was investigated by Western blotting.Results: Matrine significantly inhibited HOS cell proliferation, promoted HOS cell apoptosis, and arrested HOS cells in the G1 phase of the cell cycle. Both wound healing and Transwell invasion assays showed that matrine inhibited HOS cell migration and invasion. Western blotting results showed that matrine inhibited the activation of the MAPK/ERK signaling pathway. We found that matrine also downregulated Bcl-2 expression, which may be related to protein synthesis inhibition.Matrine can inhibit the proliferation of HOS cells, arrest HOS cells in the G1 phase, and promote HOS cell apoptosis through the MAPK/ERK signaling pathway.

    Keywords: matrine, Osteosarcoma, MAPK/ERK signaling, Antitumor effects, Pathway

    Received: 15 Nov 2023; Accepted: 23 Jul 2024.

    Copyright: © 2024 Huang, Zeng, Ruan, Lei, Zhang and Cao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Jun Zeng, Department of Anesthesiology, Shiyan People's Hospital, Shiyan, China
    Siyuan Ruan, Hubei University of Medicine, Shiyan, China
    Zhuolin Lei, Hubei University of Medicine, Shiyan, China
    Hong Cao, Hubei University of Medicine, Shiyan, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.