AUTHOR=An Ho Jung , Partha Mira A. , Lee HoJoon , Lau Billy T. , Pavlichin Dmitri S. , Almeda Alison , Hooker Anna C. , Shin Giwon , Ji Hanlee P. 

TITLE=Tumor-associated microbiome features of metastatic colorectal cancer and clinical implications

JOURNAL=Frontiers in Oncology

VOLUME=13

YEAR=2024

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2023.1310054

DOI=10.3389/fonc.2023.1310054

ISSN=2234-943X

ABSTRACT=<sec><title>Background</title><p>Colon microbiome composition contributes to the pathogenesis of colorectal cancer (CRC) and prognosis. We analyzed 16S rRNA sequencing data from tumor samples of patients with metastatic CRC and determined the clinical implications.</p></sec><sec><title>Materials and methods</title><p>We enrolled 133 patients with metastatic CRC at St. Vincent Hospital in Korea. The V3-V4 regions of the 16S rRNA gene from the tumor DNA were amplified, sequenced on an Illumina MiSeq, and analyzed using the DADA2 package.</p></sec><sec><title>Results</title><p>After excluding samples that retained &lt;5% of the total reads after merging, 120 samples were analyzed. The median age of patients was 63 years (range, 34–82 years), and 76 patients (63.3%) were male. The primary cancer sites were the right colon (27.5%), left colon (30.8%), and rectum (41.7%). All subjects received 5-fluouracil-based systemic chemotherapy. After removing genera with &lt;1% of the total reads in each patient, 523 genera were identified. Rectal origin, high CEA level (≥10 ng/mL), and presence of lung metastasis showed higher richness. Survival analysis revealed that the presence of <italic>Prevotella</italic> (<italic>p</italic> = 0.052), <italic>Fusobacterium</italic> (<italic>p</italic> = 0.002), <italic>Selenomonas</italic> (<italic>p</italic>&lt;0.001), <italic>Fretibacterium</italic> (<italic>p</italic> = 0.001), <italic>Porphyromonas</italic> (<italic>p</italic> = 0.007), <italic>Peptostreptococcus</italic> (<italic>p</italic> = 0.002), and <italic>Leptotrichia</italic> (<italic>p</italic> = 0.003) were associated with short overall survival (OS, &lt;24 months), while the presence of <italic>Sphingomonas</italic> was associated with long OS (<italic>p</italic> = 0.070). From the multivariate analysis, the presence of <italic>Selenomonas</italic> (hazard ratio [HR], 6.35; 95% confidence interval [CI], 2.38–16.97; <italic>p</italic>&lt;0.001) was associated with poor prognosis along with high CEA level.</p></sec><sec><title>Conclusion</title><p>Tumor microbiome features may be useful prognostic biomarkers for metastatic CRC.</p></sec>