AUTHOR=Friedrich Paola , Mercado Nataly , Echeandia-Abud Naomi , Guerrero-Gomez Karla , González-Zamorano Margarita , López-Ruíz Mayra Ivette , Portillo-Zavala Claudia Selene , García-Segura Laura Dolores , Reynoso-Gutiérrez Mariana , López-Facundo Norma Araceli , Cárdenas-Pedraza Daniela , Valois-Escamilla María Guadalupe , Mera-González Alma Beatriz , Covarrubias-Zapata Daniela , Vollbrechtshausen-Castelán Lilia Adriana , Loeza-Oliva José de Jesús , Garay-Sánchez Sergio Antonio , Moreno-Serrano Julio , Mendoza-Sánchez Patricia , Casillas-Toral Paola , Sandoval-Cabrera Antonio , Gutiérrez-Martínez Itzel , Jiménez-Osorio Mariana Isabel , Arce-Cabrera Daniela , Aguilar-Escobar Dinora Virginia , González-Montalvo Pablo Miguel , Romo-Rubio Hugo Antonio TITLE=Securing access to a comprehensive diagnostic panel for children with suspected acute lymphoblastic leukemia: Results from the Mexico in Alliance with St. Jude “Bridge Project” JOURNAL=Frontiers in Oncology VOLUME=13 YEAR=2024 URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2023.1286278 DOI=10.3389/fonc.2023.1286278 ISSN=2234-943X ABSTRACT=Background

The “Bridge Project” is a Mexico in Alliance with St. Jude (MAS) initiative developed in 2019 to improve access, accuracy, and timeliness of specialized diagnostic studies for patients with suspected acute lymphoblastic leukemia (ALL). The project strategy relies on service centralization to improve service delivery, biological characterization, risk-group classification, and support proper treatment allocation.

Methods

This is an ongoing prospective multisite intersectoral quality improvement (QI) project available to all patients 0-18 years of age presenting with suspected ALL to the 14 actively participating institutions in 12 Mexican states. Institutions send specimens to one centralized laboratory. From a clinical standpoint, the project secures access to a consensus-derived comprehensive diagnostic panel. From a service delivery standpoint, we assess equity, timeliness, effectiveness, and patient-centeredness. From an implementation science standpoint, we document feasibility, utility, and appropriateness of the diagnostic panel and centralized approach. This analysis spans from July 2019 to June 2023.

Results

612 patients have accessed the project. The median age was 6 years (IQR 3-11), and 53% were males. 94% of the specimens arrived within 48 hours, which documents the feasibility of the centralized model, and 100% of the patients received precise and timely diagnostic results, which documents the effectiveness of the approach. Of 505 (82.5%) patients with confirmed ALL, 463/505 (91.6%) had B-cell ALL, and 42/505 (8.3%) had T-cell ALL. High-hyperdiploidy was detected by DNA index in 36.6% and hypodiploidy in 1.6%. 76.6% of the patients had conclusive karyotype results. FISH studies showed t(12;21) in 15%, iAMP21 in 8.5%, t(1;19) in 7.5%, t(4;11) in 4.2%, t(9;22) in 3.2%, del(9)(p21) in 1.8%, and TRA/D (14)(q11.2) rearrangement in 2.4%. Among B-cell ALL patients, 344/403 (85.1%) had Day 15 MRD<1% and 261/305 (85.6%) Day 84 MRD<0.01. For T-cell ALL patients 20/28 (71.4%) had Day 29 MRD<0.01% and 19/22 (86.4%) Day 84 MRD<0.01%.

Conclusions

By securing access to a standardized consensus-derived diagnostic panel, the Bridge Project has allowed better characterization of childhood ALL in Mexico while producing unprecedented service improvements and documenting key implementation outcomes. We are using these results to inform iterative changes to the diagnostic panel and an associated treatment guideline (MAS-ALL18).