AUTHOR=Chu Xiaojie , Li Wei , Hines Margaret G. , Lyakhov Ilya , Mellors John W. , Dimitrov Dimiter S. 

TITLE=Human antibody VH domains targeting uPAR as candidate therapeutics for cancers

JOURNAL=Frontiers in Oncology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2023.1194972

DOI=10.3389/fonc.2023.1194972

ISSN=2234-943X

ABSTRACT=<p>The high expression of uPAR has been linked to tumor progression, invasion, and metastasis in several types of cancer. Such overexpression of uPAR makes it a potential target for immunotherapies across common cancers such as breast, colorectal, lung, ovarian cancer, and melanoma. In our study, two high-affinity and specific human V<sub>H</sub> domain antibody candidates, designed as clones 3 and 115, were isolated from a phage-displayed human V<sub>H</sub> antibody library. Domain-based bispecific T- cell engagers (DbTE) based on these two antibodies exhibited potent killing of uPAR-positive cancer cells. Thus, these two anti-uPAR domain antibodies are promising candidates for treating uPAR positive cancers.</p>