AUTHOR=Qiu Wenli , Xie Hui , Chen Haibin , Zhou Hongli , Wang Zhongqiu , Zhou Hongguang 

TITLE=Integrated gut microbiota and metabolome analysis reveals the mechanism of Xiaoai Jiedu recipe in ameliorating colorectal cancer

JOURNAL=Frontiers in Oncology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2023.1184786

DOI=10.3389/fonc.2023.1184786

ISSN=2234-943X

ABSTRACT=<sec><title>Introduction</title><p>Xiaoai Jiedu recipe (XJR), a classical prescription of traditional Chinese medicine (TCM), has been clinically proven to be effective in ameliorating colorectal cancer (CRC). However, its exact mechanism of action is still elusive, limiting its clinical application and promotion to a certain extent. This study aims to evaluate the effect of XJR on CRC and further illustrate mechanism underlying its action.</p></sec><sec><title>Methods</title><p>We investigated the anti-tumor efficacy of XJR <italic>in vitro</italic> and <italic>vivo</italic> experiments. An integrated 16S rRNA gene sequencing and UPLC-MS based metabolomics approach were performed to explore possible mechanism of XJR anti-CRC on the gut microbiota and serum metabolic profiles. The correlation between altered gut microbiota and disturbed serum metabolites was investigated using Pearson’s correlation analysis.</p></sec><sec><title>Results</title><p>XJR effectively displayed anti-CRC effect both <italic>in vitro</italic> and <italic>in vivo</italic>. The abundance of aggressive bacteria such as <italic>Bacteroidetes, Bacteroides</italic>, and <italic>Prevotellaceae</italic> decreased, while the levels of beneficial bacteria increased (<italic>Firmicutes</italic>, <italic>Roseburia</italic>, and <italic>Actinobacteria</italic>). Metabolomics analysis identified 12 potential metabolic pathways and 50 serum metabolites with different abundances possibly affected by XJR. Correlation analysis showed that the relative abundance of aggressive bacteria was positively correlated with the levels of <italic>Arachidonic acid</italic>, <italic>Adrenic acid</italic>, <italic>15(S)−HpETE</italic>, <italic>DL−Arginine</italic>, and <italic>Lysopc 18:2</italic>, which was different from the beneficial bacteria.</p></sec><sec><title>Discussion</title><p>The regulation of gut microbiota and related metabolites may be potential breakthrough point to elucidate the mechanism of XJR in the treatment of the CRC. The strategy employed would provide theoretical basis for clinical application of TCM.</p></sec>