AUTHOR=Meng Guangqiang , Li Mingying , Xia Yuan , Wu Yuyan , Ma Yuechan , Ji Min , Zhang Jingru , Ye Jingjing , Sun Tao , Ji Chunyan 

TITLE=Mitochondrial apoptosis-related gene polymorphisms are associated with responses to anthracycline-based chemotherapy in acute myeloid leukaemia

JOURNAL=Frontiers in Oncology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2023.1179937

DOI=10.3389/fonc.2023.1179937

ISSN=2234-943X

ABSTRACT=<sec><title>Background</title><p>Although anthracyclines are the first-line chemotherapy drugs for treating non-M3 acute myeloid leukaemia (AML), their efficacy remains limited. It is important to identify factors that influence the efficacy of anthracyclines against AML. Mitochondrial apoptosis-related genes play significant roles in the pathogenesis, treatment, and prognosis of AML.</p></sec><sec><title>Methods</title><p>We utilized the CRISPR/Cas9 screening system to find AML anthracyclines resistance related genes and several mitochondrial apoptosis-related genes, such as <italic>BCL2L11</italic>, <italic>CASP8</italic>, <italic>TP63</italic>, <italic>TP53BP2</italic>, <italic>PLAUR</italic>, <italic>SOD2</italic>, <italic>BNIP3L</italic>, and <italic>MMP9</italic>, were screened out. Then, DNA from 279 patients with AML and 321 healthy individuals were extracted and the contributions of single nucleotide polymorphisms (SNPs) within these genes to the patient’s chemotherapy response, susceptibility to AML, and overall survival were investigated.</p></sec><sec><title>Results</title><p>Our findings indicated that SNP rs4251864 in the <italic>PLAUR</italic> gene was associated with an increase in complete remission after anthracycline-based induction chemotherapy. rs4880 in <italic>SOD2</italic> was associated with the response to the second course of chemotherapy, whereas rs3789068 in <italic>BCL2L11</italic> was associated with susceptibility to AML.</p></sec><sec><title>Conclusions</title><p>Our results about the association of SNPs in mitochondrial apoptosis-related genes with the response to anthracycline-based chemotherapy in AML provide an important reference for predicting the treatment outcomes in patients with this disease.</p></sec>