AUTHOR=Zhao Jian , Zuo Weiwei , Zhang Yue , He Caiyun , Zhao Wei , Meng Tongyu 

TITLE=The polymorphism rs4705342 in the promoter of miR-143/145 is related to the risk of epithelial ovarian cancer and patient prognosis

JOURNAL=Frontiers in Oncology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2023.1122284

DOI=10.3389/fonc.2023.1122284

ISSN=2234-943X

ABSTRACT=<sec><title>Objective</title><p>To evaluate the effects of two genetic variants in the promoter of the <italic>miR-143/145</italic> cluster on the risk of epithelial ovarian cancer (EOC) and the prognosis of EOC patients.</p></sec><sec><title>Study design</title><p>Genotypes were determined by the polymerase chain reaction and ligase detection reaction method in 563 EOC patients and 576 healthy women. The expression of miR-143 and miR-145 were detected by quantitative real-time polymerase chain reaction (qRT–PCR) in fifty-two EOC tissues.</p></sec><sec><title>Results</title><p>The rs4705342 CC genotype frequencies in EOC patients were higher than those in the controls (<italic>P</italic> = 0.014). Furthermore, the CC genotype of rs4705342 was associated with an advanced FIGO stage of EOC patients (<italic>P</italic> = 0.046). Patients with the rs4705342 CC genotype had shorter progression-free survival (PFS) and overall survival (OS) times than those carrying the TT genotype in multivariable analysis adjusting for clinical variables (HR = 1.30, 95% CI = 1.04-1.62, <italic>P</italic> = 0.020; HR = 1.33, 95% CI = 1.05-1.70, <italic>P</italic> = 0.020). In addition, the miR-145 levels were lower in EOC tissues with the rs4705342 CC genotype than in those with the TT genotype (<italic>P</italic> = 0.005).</p></sec><sec><title>Conclusion</title><p>The CC genotype of rs4705342 was related to an increased risk of EOC and poor prognosis of EOC patients, and rs4705342 may serve as a molecular marker for predicting the development of EOC and the clinical outcome of EOC patients.</p></sec>