AUTHOR=Shi Yubo , Tian Yunyun , Sun Xiangran , Qiu Yonglong , Zhao Yingchun 

TITLE=Silencing circOMA1 Inhibits Osteosarcoma Progression by Sponging miR-1294 to Regulate c-Myc Expression

JOURNAL=Frontiers in Oncology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2022.889583

DOI=10.3389/fonc.2022.889583

ISSN=2234-943X

ABSTRACT=<sec><title>Background</title><p>Several studies have reported that circRNAs have a crucial function in the tumorigenesis of various cancers. However, the expression and function of circOMA1 in osteosarcoma is unknown.</p></sec><sec><title>Methods</title><p>circOMA1 was identified through bioinformatics analysis. qRT-PCR was used to assess the expressions of circOMA1, miR-1294, and c-Myc in osteosarcoma tissues. Further, we performed functional experiments to explore the biological function of circOMA1 in osteosarcoma. Moreover, a luciferase reporter assay, RNA immunoprecipitation (RIP), and fluorescence <italic>in situ</italic> hybridisation (FISH) assay were performed to demonstrate the association between circOMA1 and miR-1294.</p></sec><sec><title>Results</title><p>circOMA1 exhibited considerable upregulation in osteosarcoma tissues compared with adjacent normal tissues. Silencing circOMA1 suppressed osteosarcoma progression <italic>in vitro</italic> and <italic>in vivo</italic>. Mechanically, circOMA1 functioned as a sponge of miR-1294 to upregulate c-Myc expression.</p></sec><sec><title>Conclusion</title><p>circOMA1 played the role of an oncogene in osteosarcoma and promoted osteosarcoma progression by mediating the miR-1294/c-Myc pathway, which might be a new target for treating osteosarcoma.</p></sec>