This article is a correction to:
Doxycycline Inhibits Cancer Stem Cell-Like Properties via PAR1/FAK/PI3K/AKT Pathway in Pancreatic Cancer
Huijuan Liu1,2,3*†
Honglian Tao1,2†
Hongqi Wang1,2†
Yuyan Yang1,2Ru Yang1,2
Xintong Dai1,2
Xiujuan Ding1,2
Haidong Wu2
Shuang Chen2
Tao Sun1,2,4*- 1State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China
- 2Tianjin Key Laboratory of Early Druggability Evaluation of Innovative Drugs, Tianjin International Joint Academy of Biomedicine, Tianjin, China
- 3Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Tianjin Third Central Hospital, Tianjin, China
- 4Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin Institute of Digestive Disease, Tianjin, China
A Corrigendum on
Doxycycline Inhibits Cancer Stem Cell-Like Properties via PAR1/FAK/PI3K/AKT Pathway in Pancreatic Cancer
By: Liu H, Tao H, Wang H, Yang Y, Yang R, Dai X, Ding X, Wu H, Chen S and Sun T (2021) Front. Oncol. 10:619317. doi 10.3389/fonc.2020.619317
In the original article, there was a mistake in Figures 2 and 5 as published. The presented figure of siPAR1 (0 h) in Figure 2A and the presented figure of Group 30 μM (cells treated with 30 μM of doxycycline) in Figure 5E were wrongly presented in the original article. Furthermore, the annotation in the ordinate axis of the statistical figure in Figures 2B and 5E should be “invasion cells per field” not the “passed cells per field.” The corrected Figures 2 and 5 appear below.
FIGURE 2
Figure 2 PAR1 promotes EMT progression of pancreatic cancer cells. (A) Effect of PAR1 on Panc-1 and Aspc-1 cell migration potential detected using the wound healing assay. (B) Effect of PAR1 on pancreatic cancer cell invasion potential by using matrigel coated transwell assay. (C) Effect of PAR1 on the E-Cad and VIM expression detected by Western blot analysis. (D) Effect of PAR1 on the E-Cad and VIM expression detected by immunofluorescence. Data are shown as the mean ± SD (*P < 0.05, **P < 0.01).
FIGURE 5
Figure 5 Doxycycline inhibits the cancer stem cell-like properties of pancreatic cancer cells. (A) Effect of doxycycline on PAR1 activation stimulated by thrombin in pancreatic cancer cells detected by the Ca2+-mobilization assay. (B) Effect of doxycycline on pancreatic cancer cell viability after 48, 72, and 96 h treatment. (C) Effect of doxycycline on pancreatic cancer stem cell marker CD133 in Panc-1 cells. (D) Effect of doxycycline on the mammosphere formation of Panc-1 cells. (E) Effect of doxycycline on pancreatic cancer cell invasion ability. (F) Effect of doxycycline on pancreatic cancer cells migration ability. (G) Limiting dilution assay of pancreatic cancer stem cell from Panc-1 cells after treatment with doxycycline in node mice (n = 8). Cancer stem cell frequency was determined by ELDA. Data are shown as the mean ± SD (*P < 0.05, **P < 0.01).
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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Keywords: protease activation receptor 1, focal adhesion kinase, doxycycline, epithelial–mesenchymal transformation, pancreatic cancer stem cells
Citation: Liu H, Tao H, Wang H, Yang Y, Yang R, Dai X, Ding X, Wu H, Chen S and Sun T (2022) Corrigendum: Doxycycline Inhibits Cancer Stem Cell-Like Properties via PAR1/FAK/PI3K/AKT Pathway in Pancreatic Cancer. Front. Oncol. 12:830506. doi: 10.3389/fonc.2022.830506
Received: 07 December 2021; Accepted: 24 January 2022;
Published: 24 February 2022.
Edited and reviewed by:
Zhi-xiang Yuan, Southwest Minzu University, ChinaCopyright © 2022 Liu, Tao, Wang, Yang, Yang, Dai, Ding, Wu, Chen and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Tao Sun, dGFvLnN1bkBuYW5rYWkuZWR1LmNu; Huijuan Liu, bGl1aHVpanVhbnh5ekAxNjMuY29t
†These authors have contributed equally to this work