AUTHOR=Marr Kendra D. , Ignatenko Natalia A. , Warfel Noel A. , Batai Ken , Cress Anne E. , Pollock Grant R. , Wong Ava C. , Lee Benjamin R. 

TITLE=Digital image analysis using video microscopy of human-derived prostate cancer vs normal prostate organoids to assess migratory behavior on extracellular matrix proteins

JOURNAL=Frontiers in Oncology

VOLUME=12

YEAR=2023

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2022.1083150

DOI=10.3389/fonc.2022.1083150

ISSN=2234-943X

ABSTRACT=<p>The advent of perpetuating living organoids derived from patient tissue is a promising avenue for cancer research but is limited by difficulties with precise characterization. In this brief communication, we demonstrate <italic>via</italic> time-lapse imaging distinct phenotypes of prostate organoids derived from patient material– without confirmation of cellular identity. We show that organoids derived from histologically normal tissue more readily spread on a physiologic extracellular matrix (ECM) than on pathologic ECM (p&lt;0.0001), while tumor-derived organoids spread equally on either substrate (p=0.2406). This study is an important proof-of-concept to defer precise characterization of organoids and still glean information into disease pathology.</p>