AUTHOR=Li Jie , Xu Anping , Xie Weijie , Li Bowen , Yan Cunliang , Xia Yong , Liang Chao , Ji Ling 

TITLE=MALDI-TOF-MS for rapid screening analysis of M-protein in serum

JOURNAL=Frontiers in Oncology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2022.1073479

DOI=10.3389/fonc.2022.1073479

ISSN=2234-943X

ABSTRACT=<p>Monoclonal immunoglobin (M-protein) is a serum biomarker for the diagnosis of plasma cell dyscrasias. Despite limitation of analytical sensitivity and resolution, serum protein electrophoresis and immunofixation electrophoresis are still the front-line tests for the detection of M-proteins. Herein, we developed a MALDI-TOF Mass spectrometry-based method for the screening test of M-proteins in human serum. Based on the unique mass signature of different immunoglobin isotypes, M-Proteins could be rapidly identified and typed. The method demonstrated with high analytical performance and throughput, rapid and simple, which could be a new choice for the diagnosis of plasma cell dyscrasias.</p>