AUTHOR=Kocsis Zsuzsa S. , Farkas Gyöngyi , Bajcsay András , Kun-Gazda Márta , Lövey József , Ostoros Gyula , Pócza Tamás , Herein András , Ladányi Katalin , Székely Gábor , Markóczy Zsolt , Takácsi-Nagy Zoltán , Polgár Csaba , Juranyi Zsolt 

TITLE=Chromosomal Aberrations in Blood Lymphocytes as Predictors of Respiratory Function After Stereotactic Lung Irradiation

JOURNAL=Frontiers in Oncology

VOLUME=11

YEAR=2022

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.829972

DOI=10.3389/fonc.2021.829972

ISSN=2234-943X

ABSTRACT=<p>Due to the profound difference in radiosensitivity of patients and various side effects caused by this phenomenon, a radiosensitivity marker is needed. Prediction by a marker may help personalise the treatment. In this study, we tested chromosomal aberrations (CA) of <italic>in vitro</italic> irradiated blood as predictor of pulmonary function decrease of nonsmall cell lung cancer (NSCLC) patients and also compared it with the CAs in the blood of irradiated patients. Peripheral blood samples were taken from 45 lung cancer patients before stereotactic radiotherapy (SBRT) and immediately after the last fraction and 3, 6, 9, 12, 15, 18, 21, and 24 months later. Respiratory function measurements were performed at the same time. Diffusing capacity of lung for carbon monoxide (DLCO), forced vital capacity (FVC), forced expiratory volume in 1 s (FEV1s), and FEV1s/FVC (FEV1%) were monitored. Metaphase preparations of lymphocytes were made with standard procedures, and chromosome aberrations were analysed. In our cohort, the 36-month local relapse-free survival was 97.4%, and the distant metastasis-free survival was 71.5% at 36 months. There was no change in the mean of the pulmonary function tests (PFTs) after the therapy. However, there was a considerable variability between the patients. Therefore, we subtracted the baseline and normalised the PFT values. There were significant decreases at 12–24 months in relative FEV1s and relative FEV1%. The tendentious decrease of the PFTs could be predicted by the <italic>in vitro</italic> chromosome aberration data. We also found connections between the <italic>in vitro</italic> and <italic>in vivo</italic> CA values (i.e., dicentrics plus rings after 3 Gy irradiation predicts dicentric-plus-ring value directly after the radiotherapy/V<sub>54 Gy</sub> (<italic>p</italic> = 0.001 24.2%)).</p><p>We found that—after further validation—chromosome aberrations resulted from <italic>in vitro</italic> irradiation before radiotherapy can be a predictive marker of pulmonary function decrease after lung irradiation.</p>