AUTHOR=Rong Xiaoxiang , Lv Jinyu , Liu Yantan , Wang Zhaojun , Zeng Dongqiang , Li Yuedan , Li Shaowei , Wu Jianhua , Shen Zheyu , Shi Min , Liao Wangjun , Wu Zhenzhen , Wang Chunlin 

TITLE=PET/CT Imaging of Activated Cancer-Associated Fibroblasts Predict Response to PD-1 Blockade in Gastric Cancer Patients

JOURNAL=Frontiers in Oncology

VOLUME=11

YEAR=2022

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.802257

DOI=10.3389/fonc.2021.802257

ISSN=2234-943X

ABSTRACT=<sec><title>Background</title><p>Promising development in immune checkpoint blockade (ICB) therapy has shown remarkable results in the treatment of gastric cancer (GC). However, the objective response rate in GC remains unsatisfactory. Noninvasive imaging to predict responses to ICB therapy <italic>via</italic> tumor microenvironment (TME) assessment is needed. Accordingly, this study aimed to evaluate the role of <sup>68</sup>Ga-FAPI-04 PET/CT in the assessment of the immunosuppressive TME in GC and to cross-correlate imaging findings with responses to ICB therapy.</p></sec><sec><title>Methods</title><p>The correlation between fibroblast-activation-protein (FAP) expression and immunosuppressive cell infiltration was analyzed using The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) database, and GC tissue microarrays. To characterize the TME, TMEscores were calculated based on RNA-seq data from four GC patients. A total of 21 patients with GC underwent <sup>68</sup>Ga-FAPI-04 PET/CT before ICB treatment, and two of them were imaged after ICB therapy.</p></sec><sec><title>Results</title><p>FAP expression was found to be closely correlated with poor prognosis and infiltration of immunosuppressive cells, including myeloid-derived suppressor cells (MDSCs), exhausted T cells, and regulatory T cells (Tregs) in GC. We also found a strong relationship (<italic>R</italic><sup>2</sup> = 0.9678, <italic>p</italic> = 0.0162) between <sup>68</sup>Ga-FAPI-04 uptake and TMEscore. Further analyses indicated that high <sup>68</sup>Ga-FAPI-04 uptake was correlated with reduced therapeutic benefits from ICB therapy.</p></sec><sec><title>Conclusions</title><p><sup>68</sup>Ga-FAPI-04 PET/CT may be used to noninvasively image the cancer-associated fibroblasts immunosuppressive TME <italic>in vivo</italic> and also potentially serve as a predictive biomarker of survival and antitumor immune response among patients who received ICB therapies.</p></sec>