AUTHOR=Abba Martin C. , Fabre María Laura , Lee Jaeho , Tatineni Pradeep , Kil Hyunsuk , Aldaz C. Marcelo 

TITLE=HOTAIR Modulated Pathways in Early-Stage Breast Cancer Progression

JOURNAL=Frontiers in Oncology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.783211

DOI=10.3389/fonc.2021.783211

ISSN=2234-943X

ABSTRACT=<p>The long-non-coding HOX transcript antisense intergenic RNA (<italic>HOTAIR</italic>) was identified as significantly upregulated in breast ductal carcinoma <italic>in situ</italic> (DCIS). The aim of this study was to characterize the phenotypic effects and signaling pathways modulated by <italic>HOTAIR</italic> in early-stage breast cancer progression. We determined that <italic>HOTAIR</italic> induces premalignant phenotypic changes by increasing cell proliferation, migration, invasion and <italic>in vivo</italic> growth in normal and DCIS breast cell lines. Transcriptomic studies (RNA-seq) identified the main signaling pathways modulated by <italic>HOTAIR</italic> which include bioprocesses related to epithelial to mesenchymal transition, cell migration, extracellular matrix remodeling and activation of several signaling pathways (HIF1A, AP1 and FGFR). Similar pathways were identified as activated in primary invasive breast carcinomas with <italic>HOTAIR</italic> over-expression. We conclude that <italic>HOTAIR</italic> over-expression behaves as a positive regulator of cell growth and migration both in normal and DCIS breast cells involved with early-stage breast cancer progression.</p>