Corrigendum: A Novel Invadopodia-Specific Marker for Invasive and Pro-Metastatic Cancer Stem Cells

A Corrigendum on
A Novel Invadopodia-Specific Marker for Invasive and Pro-Metastatic Cancer Stem Cells

By Huang S-S, Liao W-Y, Hsu C-C, Chan T-S, Liao T-Y, Yang P-M, Chen L-T, Sung S-Y and Tsai KK (2021). Front. Oncol. 11:638311. doi: 10.3389/fonc.2021.638311

In the original article, there was a mistake in the legend for Figure 3 as published. The name of the mouse strain described in (A) and (C) was mis-spelled as “NOC/SCID”, which should be “NOD/SCID”. Appended below is the corrected legend.

FIGURE 3

Figure 3 sENO1⁺ CSCs are highly pro-metastatic. (A) Representative BLI of NOD/SCID mice receiving an intra-splenic injection of sENO1⁺ CSCs (represented by CD90⁺ NCI-N87 cells), sENO1^- CSCs (CD90^- NCI-N87 cells), and non-CSCs (represented by CD90^- cells). at the indicated time following cell inoculation. (B) Tumor bulk quantified as BLI normalized photon counts as a function of time. Error bars represent mean ± SEM from one experiment (n = 8 mice per group). Unpaired t-test was performed throughout where **p < 0.01 versus non-CSCs. (C) Representative BLI of NOD/SCID mice receiving intra-splenic injection of sENO1⁺CSCs (represented by CD90⁺ MKN-45 cells), sENO1^- CSCs (CD90^- MKN-45 cells) and non-CSCs (represented by CD90^- cells). at the indicated time following cell inoculation. (D) Tumor bulk quantified as BLI normalized photon counts as a function of time. Error bars represent mean ± SEM from one experiment (n = 8 mice per group). Unpaired t-test was performed throughout where *p < 0.05 versus non-CSCs.

In the original article, there was a mistake in the legend for Figure 4 as published. We mislabeled the name of the cell line in (B) as “NCI-N87”, which should be “AGS” as described in the main text. Appended below is the corrected legend.

FIGURE 4

Figure 4 ENO1 is expressed on the invadopodial surface of CSCs. (A) Confocal views of PAC CSCs (represented by CD44⁺CD133⁺ PC-3 cells) showing the cross-section of invadopodia structures (represented by cortactin⁺F-acin⁺ puncta) with the colocalized surface ENO1 (sENO1; green), cortactin (red), and F-actin (magenta) that penetrate into the underlying gelatin matrix. Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI; blue). Scale, 10 μm. (B) Top, a pie chart showing the percentage of sENO1⁺ invadopodia per PC-3 CSC. Bottom, a pie chart showing the percentage of sENO1⁺ invadopodia per GAC AGS CSC (represented by CD90⁺ AGS cells). (C) Left, representative three-dimensional (3D) reconstructed confocal image of CD44⁺CD133⁺ PC-3 CSCs showing the colocalization of sENO1 (green) and cortactin (red) at the ventral side of cell. Scale, 8 μm. Right upper, digital zoom-in image from serial Z sections (yellow rectangle) showing the spatial colocalization of sENO1 (green) and cortactin (red) at invadopodia. Scale, 5 μm. Right lower, the orthogonal view of the magnified areas (yellow squares at top) shown the distribution and localization of sENO1 and cortactin at the base of invadopodia. 3D rendered images of the invadopodia (arrows) were processed by using Imaris software. Scale, 1 μm.

In the original article, there was a mistake in Figure 4 as published. We mislabeled the name of the cell line as “NCI-N87” at the lower panel of Figure 4B, which should be “AGS” as described in the main text. We mark the right name in the red rectangle in the corrected figure below.

The authors apologize for these errors and state that these do not change the scientific conclusions of the article in any way.