AUTHOR=Huang Sheng-Kai , Ni Ruo-Xuan , Wang Wen-Jie , Wang Di , Zhao Mei , Lei Cheng-Zhi , Sun Xiao-Jie , Huang Chang-Zhi , Bai Ping , Che Yi-Qun , Xu Jian-Ping 

TITLE=Overexpression of LINC00673 Promotes the Proliferation of Cervical Cancer Cells

JOURNAL=Frontiers in Oncology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.669739

DOI=10.3389/fonc.2021.669739

ISSN=2234-943X

ABSTRACT=<sec><title>Objective</title><p>To study the expression of LINC00673 in cervical cancer and cervical intraepithelial neoplasia (CIN) and to explore the role of LINC00673 in the development of cervical cancer.</p></sec><sec><title>Methods</title><p>The expression of LINC00673 in serum from cervical cancer patients, CIN patients, and healthy participants was detected by RT-qPCR. The function of LINC00673 in cervical cancer cells was analyzed using <italic>in vitro</italic> and <italic>in vivo</italic> experiments.</p></sec><sec><title>Results</title><p>Our results revealed that serum LINC00673 levels were highest in cervical cancer patients, followed by patients with CIN and healthy controls. <italic>In vitro</italic> experiments demonstrated that overexpression of LINC00673 enhanced the proliferation and cell cycle progression of HeLa and SiHa cells. <italic>In vivo</italic> experiments showed that the tumor weight and volume of nude mice subcutaneously injected with LINC00673-overexpressing HeLa cells were larger than those of nude mice injected with control cells (<italic>P</italic> &lt; 0.05). Western blotting showed that cell cycle-related proteins cyclin A2 and cyclin E and interstitial-associated proteins Snail and N-cadherin were upregulated and p53 signaling pathway-related proteins were downregulated in LINC00673-overexpressing HeLa and SiHa cells.</p></sec><sec><title>Conclusion</title><p>LINC00673 plays an important role in the development of cervical cancer and may serve as a new therapeutic target for cervical cancer.</p></sec>