AUTHOR=Kennedy Susan A. , Annett Stephanie L. , Dunne Margaret R. , Boland Fiona , O’Neill Linda M. , Guinan Emer M. , Doyle Suzanne L. , Foley Emma K. , Elliott Jessie A. , Murphy Conor F. , Bennett Annemarie E. , Carey Michelle , Hillary Daniel , Robson Tracy , Reynolds John V. , Hussey Juliette , O’Sullivan Jacintha 

TITLE=Effect of the Rehabilitation Program, ReStOre, on Serum Biomarkers in a Randomized Control Trial of Esophagogastric Cancer Survivors

JOURNAL=Frontiers in Oncology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.669078

DOI=10.3389/fonc.2021.669078

ISSN=2234-943X

ABSTRACT=<sec><title>Background</title><p>The Rehabilitation Strategies Following Esophagogastric cancer (ReStOre) randomized control trial demonstrated a significant improvement in cardiorespiratory fitness of esophagogastric cancer survivors. This follow-up, exploratory study analyzed the biological effect of exercise intervention on levels of 55 serum proteins, encompassing mediators of angiogenesis, inflammation, and vascular injury, from participants on the ReStOre trial.</p></sec><sec><title>Methods</title><p>Patients &gt;6 months disease free from esophagogastric cancer were randomized to usual care or the 12-week ReStOre program (exercise training, dietary counselling, and multidisciplinary education). Serum was collected at baseline (T0), post-intervention (T1), and at 3-month follow up (T2). Serum biomarkers were quantified by enzyme-linked immunosorbent assay (ELISA).</p></sec><sec><title>Results</title><p>Thirty-seven patients participated in this study; 17 in the control arm and 20 in the intervention arm. Exercise intervention resulted in significant alterations in the level of expression of serum IP-10 (mean difference (MD): 38.02 (95% CI: 0.69 to 75.35)), IL-27 (MD: 249.48 (95% CI: 22.43 to 476.53)), and the vascular injury biomarkers, ICAM-1 (MD: 1.05 (95% CI: 1.07 to 1.66)), and VCAM-1 (MD: 1.51 (95% CI: 1.04 to 2.14)) at T1. A significant increase in eotaxin-3 (MD: 2.59 (95% CI: 0.23 to 4.96)), IL-15 (MD: 0.27 (95% CI: 0 to 0.54)) and decrease in bFGF (MD: 1.62 (95% CI: -2.99 to 0.26)) expression was observed between control and intervention cohorts at T2 (p&lt;0.05).</p></sec><sec><title>Conclusions</title><p>Exercise intervention significantly altered the expression of a number of serum biomarkers in disease-free patients who had prior treatment for esophagogastric cancer.</p></sec><sec><title>Impact</title><p>Exercise rehabilitation causes a significant biological effect on serum biomarkers in esophagogastric cancer survivors.</p></sec><sec><title>Clinical Trial Registration</title><p><uri xlink:href="http://www.clinicaltrials.gov" xmlns:xlink="http://www.w3.org/1999/xlink">ClinicalTrials.gov</uri> (NCT03314311).</p></sec>