AUTHOR=Pang Bo , Wang Yong , Chang Xiaoyan 

TITLE=RETRACTED: A Novel Tumor Suppressor Gene, ZNF24, Inhibits the Development of NSCLC by Inhibiting the WNT Signaling Pathway to Induce Cell Senescence

JOURNAL=Frontiers in Oncology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.664369

DOI=10.3389/fonc.2021.664369

ISSN=2234-943X

ABSTRACT=<sec><title>Objective</title><p>Understanding the characteristics of tumor suppressor genes (TSGs) is of great significance for the development of new targeted treatment strategies for non-small cell lung cancer (NSCLC). Therefore, this present article is to explore the underlying molecular mechanism of ZFN24 inhibiting the development of NSCLC.</p></sec><sec><title>Methods</title><p>We performed RT-PCR and Western blotting for evaluating associated RNA and protein expression. CCK8, colony forming and sphere-forming assays were used to evaluate the proliferation and stemness of NSCLC cells. NSCLC cell senescence was examined by <italic>β</italic>-galactosidase staining assay. Luciferase assay was performed to evaluate <italic>β</italic>-catenin transcriptional activity. The effect of ZNF24 on NSCLC cells <italic>in vivo</italic> was evaluated by the xenograft tumor experiment.</p></sec><sec><title>Results</title><p>Ectopic expression of ZNF24 significantly inhibited cell viability, colony forming ability, and stemness of NSCLC cells. WNT signaling pathway was inhibited by ZNF24 resulting in NSCLC cell senescence. <italic>β</italic>-catenin transcriptional activity was significantly inhibited by ZNF24 (P &lt; 0.05). Ectopic expression of ZNF24 significantly inhibited xenotransplant tumors growth <italic>in vivo</italic> (P &lt; 0.05).</p></sec><sec><title>Conclusion</title><p>ZNF24 could notably inhibit the development of NSCLC by inhibiting the WNT signaling pathway.</p></sec>