Efficacy and Safety of Astragalus-Containing Traditional Chinese Medicine Combined With Platinum-Based Chemotherapy in Advanced Gastric Cancer: A Systematic Review and Meta-Analysis

Background: Astragalus-containing traditional Chinese medicine (TCM) is widely used as adjunctive treatment to platinum-based chemotherapy (PBC) in patients with advanced gastric cancer (AGC) in China. However, evidence regarding its efficacy remains limited. This study aimed to evaluate the efficacy and safety of Astragalus-containing TCM combined with PBC in AGC treatment.

Methods: We searched for literature (up to July 19, 2020) in eight electronic databases. The included studies were reviewed by two researchers. The main outcomes were the objective response rate (ORR), disease control rate (DCR), survival rate, quality of life (QOL), adverse drug reactions (ADRs), and peripheral blood lymphocyte levels. The effect estimate of interest was the risk ratio (RR) or mean difference (MD) with 95% confidence intervals (CIs). Trial sequential analysis (TSA) was used to detect the robustness of the primary outcome and to calculate the required information size (RIS). Certainty of the evidence was assessed using the GRADE profiler.

Results: Results based on available literature showed that, compared with patients treated with PBC alone, those treated with Astragalus-containing TCM had a better ORR (RR: 1.24, 95% CI: 1.15–1.34, P < 0.00001), DCR (RR: 1.10, 95% CI: 1.06–1.14, P < 0.00001), 1-year survival rate (RR: 1.41, 95% CI: 1.09–1.82, P = 0.009), 2-year survival rate (RR: 3.13, 95% CI: 1.80–5.46, P < 0.0001), and QOL (RR: 2.03, 95% CI: 1.70–2.43, P < 0.00001 and MD: 12.39, 95% CI: 5.48–19.30, P = 0.0004); higher proportions of CD3⁺ T cells and CD3⁺ CD4⁺ T cells; higher ratio of CD4⁺/CD8⁺ T cells; nature killer cells; and lower incidence of ADRs. Subgroup analysis showed that both oral and injection administration of Astragalus-containing TCM increased tumor response. Whether treatment duration was ≥8 weeks or <8 weeks, Astragalus-containing TCM could increase tumor response in AGC patients. Furthermore, Astragalus-containing TCM combined with oxaliplatin-based chemotherapy could increase the ORR and DCR; when with cisplatin, it could only increase the ORR.

Conclusion: Current low to moderate evidence revealed that Astragalus-containing TCM combined with PBC had better efficacy and less side effects in the treatment of AGC; however, more high-quality randomized studies are warranted.

Systematic Review Registration: PROSPERO, identifier CRD42020203486.

Introduction

As a global health problem, gastric cancer remains the third most common cause of cancer-related death worldwide accounting for 8.2% of cancer-related deaths in 2018, equating to 1 in every 12 deaths (1). Unfortunately, a considerable number of patients with gastric cancer are diagnosed at advanced stage, meaning that the opportunity for surgical treatment has been lost (2). Platinum-based chemotherapy (PBC) is widely used in the treatment of advanced gastric cancer (AGC), and platinum (cisplatin or oxaliplatin) plus fluoropyrimidine regimen is suggested as the first-line chemotherapy regimen according to the National Comprehensive Cancer Network (NCCN) guidelines (version 2.2020) (3, 4). However, in clinical practice, the adverse events of and chemotherapy resistance to PBC have exposed the limitations of PBC, prompting researchers and clinicians to pay more attention to the study of alternative and complementary therapies.

In China, Chinese herb medicine used in Traditional Chinese Medicine (TCM) is frequently combined with chemotherapy in the treatment of AGC; specifically, it has been shown to improve the efficacy of chemotherapy and reduce its side effects (5–7). Astragalus (also called Huangqi in Chinese) is sourced from the leguminous plant Astragalus membranaceus (Fisch.), which has been recognized as one of the primary tonic herbs in TCM over 2,000 years and is widely used in the treatment of malignant tumors in China.

Many researchers have examined the effectiveness and safety of Astragalus-containing TCM combined with PBC in the treatment of colorectal cancer and non-small-cell lung cancer (8–11). However, the effects of Astragalus-containing Chinese medicines plus PBC in AGC treatment have not been systematically assessed.

In recent decades, a large number of trials on the use of Astragalus-containing Chinese herbal therapies combined with PBC for AGC treatment have been published. This study aimed to provide a systematic analysis of the results obtained in these studies in order to understand the safety and efficacy of Astragalus-containing TCM in combination with PBC during AGC treatment. To do so, we performed a meta-analysis of the available studies on Astragalus-containing TCM combined with PBC in order to further clinically investigate the effects on safety and efficacy.

Methods

This study was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The protocol has been registered on PROSPERO, under the number CRD42020203486.

Search Strategy

PubMed, EMBASE, Cochrane Central Register of Controlled Trials (CENTRAL), clinicaltrials.gov, Chinese Biomedical Literature database (CBM), China Academic Journals (CNKI), Chinese Science and Technology Journals (CQVIP), and Wanfang Database were searched systematically for all articles published from inception to July 19, 2020. The following search terms were used: ([gastr* OR stomach* OR digest* OR epigastr*] AND [carcin* OR cancer* OR neoplas* OR tumour* OR tumor* OR growth* OR adenocarcin* OR malig*]) AND (Astragalus OR radix astragali OR huang qi OR huangqi). All retrievals were implemented using MeSH and free words (the detailed search strategy is available in Supplementary 1). The languages were restricted to Chinese and English.

Inclusion and Exclusion Criteria

Inclusion criteria: (1) All studies were randomized controlled trials (RCTs) or quasi-RCTs. (2) Patients who had TNM stage III-IV AGC and were diagnosed using the histopathological and cytological diagnostic criteria. (3) The experimental group of patients was given Astragalus-based herbal therapy combined with PBC. Any form of Astragalus (Huang qi) preparation, including water decoction, extracts, granules, or injection, among other forms, regardless of administration route, were included. The control group of patients was given PBC alone. (4) Outcomes were identified as the tumor response, survival rate, QOL, ADRs, and peripheral blood lymphocytes levels, and at least one of these outcomes was reported; and (5) for repeated publication studies, we selected the data with the most comprehensive report and the longest follow-up.

Exclusion Criteria: (1) Patients who underwent radiotherapy, chemotherapy, or other antitumor therapy within 1 month before treatment; (2) patients with severe infection, other malignant tumors, and severe medical diseases; (3) the prescription of Astragalus-based herbal therapy was not fixed; (4) studies for which the data could not be extracted; and (5) the baseline data of patients in two groups were not comparable.

Study Selection and Data Extraction

Studies were selected independently by two reviewers according to the above inclusion and exclusion criteria. Two reviewers independently extracted the data. Disagreements were discussed with and resolved by the third reviewer. The following data were extracted: first author (year of publication), sample sizes, gender, age, study arm, drug delivery, treatment duration, follow-up time, outcomes, and criteria. Data presented graphically were extracted using WebPlotDigitizer (https://automeris.io/WebPlotDigitizer).

Risk of Bias Assessment

Two reviewers independently assessed the quality of the selected studies according to the Cochrane Collaboration’s tool for RCTs. Items were sorted into three categories: low risk of bias, unclear bias, and high risk of bias. The following characteristics were evaluated: Random sequence generation (selection bias), allocation concealment (selection bias), blinding of participants and personnel (performance bias), incomplete outcome data (attrition bias), selective reporting (reporting bias), and other biases. Results from these evaluations were graphed and assessed using Review Manager 5.3.

Outcome Definition

Tumor response and the survival rate were the main outcomes. Tumor response, containing the objective response rate (ORR) and disease control rate (DCR), was based on the World Health Organization (WHO) criteria (12) or Response Evaluation Criteria in Solid Tumors (RECIST) (13). Complete response (CR), partial response (PR), stable disease (SD), and progressive disease (PD) were used as indicators. The CR plus PR rates were as equal to the ORR; the CR plus PR, and SD rates were equal to the DCR.

Quality of life (QOL), adverse drug reactions (ADRs), and peripheral blood lymphocytes levels were the second outcomes. QOL was considered improved when the Karnofsky Performance Status (KPS) score was 10 points higher after treatment than before treatment. ADRs were accessed by measuring hematotoxicity (neutropenia, anemia, and thrombocytopenia), gastrointestinal toxicity (nausea and vomiting, diarrhea), hepatic and renal dysfunction, neurotoxicity, alopecia, and stomatitis, based on WHO criteria (12) or NCI Common Terminology Criteria for Adverse Events (CTCAE) (14). The peripheral blood lymphocyte levels were evaluated by measuring the T-lymphocyte subsets such as the percentage of CD3⁺, CD3⁺CD4⁺, and CD3⁺CD8⁺ T cells, as well as the CD4⁺/CD8⁺ T cells ratio and the percentage of natural killer (NK) cells.

Data Analysis

The Review Manager 5.3 and Stata V16.0 software were used in this study. The risk ratio (RR) and 95% confidence interval (CI) were used to assess dichotomous variables, while mean difference (MD) with a 95% CI was used to assess continuous variables. Statistical heterogeneity was measured by the I² statistic and the Chi-squared test. The summary RR, MD, and 95% CI were estimated by a random-effect model. P values were used to calculate outcomes, and P < 0.05 was considered statistically significant. The funnel plots and Egger’s tests were applied to examine potential publication bias when studies ≥10. In accordance with the drug delivery of Astragalus-containing TCM, PBC regimen, and treatment duration, subgroup analyses were performed to reveal the clinical heterogeneity and its influence on tumor response.

Trial sequential analysis software (TSA, version 0.9.5.10 beta) was used to determine the robustness of main outcomes and to calculate the required information size (RIS) in the meta-analysis (15). If the cumulative Z-curve stretched across the monitoring boundaries, a sufficient level of evidence for the intervention effect may have been reached and no further studies were needed. Type I and II errors were needed to perform the TSA. In this review, the RIS was estimated using α = 0.05 (two sided) with power equal to 80%.

Evidence Quality Assessment

Two reviewers independently assessed the quality of the evidence for each outcome using the GRADE approach (16). Disagreements were discussed with and resolved by the third reviewer. The quality of evidence was classified as being high, moderate, low, and very low. The quality was downgraded according to the following five domains: (1) risk of bias (if most trials showed an unclear risk, with or without high risk, but the results had good robustness, the evidence was rated down by only one level; if the results showed poor robustness, the evidence was downgraded by two levels); (2) inconsistency (statistical heterogeneity was present and the results of the sensitivity analysis had poor robustness); (3) indirectness (the participants, intervention, outcomes, or comparison of the study did not meet the objectives of this study); (4) imprecision (the sample size for each outcome was fewer than 300 cases); and (5) reporting bias (publication bias). Except for the risk of bias, evidence was downgraded by one level.

Results

Search Results

A total of 2,873 records were identified through database searching. All records were screened by two reviewers with a three-step process. Firstly, we screened the titles and removed duplicates, after which 1,829 records remained. Secondly, we included 227 full texts by reading the title or abstract. Thirdly, we evaluated the full texts and excluded 192 trials by following the inclusion and exclusion criteria. In the end, 35 eligible studies involving 2,670 stage III/IV AGC patients were included in our study (Figure 1).

FIGURE 1

Figure 1 Flow diagram of selection process (17).

Characteristics of the Included Studies

The characteristic information of the included studies is shown in Table 1. All 35 studies were single-centered and conducted in China. There were 1,572 male and 1,098 female patients, and sample sizes ranged from 30 to 240. Eighteen studies used the oral TCM administration, and 17 studies used commercial TCM injections. All clinical trials were designed to use Astragalus-containing TCM as the principal drug together with PBC. Follow-up lasted from 4 weeks to 3 years. Twenty trials reported the tumor responses according to the WHO guidelines, and 12 reported them according to RECIST guidelines. Eighteen trials reported the ADRs using WHO criteria, three using NCI CTCAE criteria, and eight using unknown criteria.

TABLE 1

Table 1 Characteristics of the included studies.

Risk of Bias

All 35 studies referred to randomization; however, only eight studies (23, 25, 29, 30, 43, 47, 50, 52) used a random number table to generate a random sequence, one study used a lottery approach (24), and two studies (35, 49) used admission order, which was inappropriate. One study used an envelope method (44) to perform the allocation concealment, while the other studies did not report the method. Only one study mentioned single blinding (25), and none of the studies adequately reported the blinding of the investigators, patients, and outcome assessors. Selective reporting existed in one study (40) that failed to completely report the DCR. One study (20) had a high risk of other bias because it was funded by the industry. The risk of bias for the individual trials is summarized in Figure 2.

FIGURE 2

Figure 2 Risk of bias summary and diagram. (A) Risk of bias summary. (B) Risk of bias diagram.

Tumor Response

Thirty-two trials with 2,454 patients reported the ORR following WHO or RECIST guidelines. The random-effects meta-analysis showed that Astragalus-based herbal therapy plus PBC enhanced the ORR, which showed a statistically significant difference (RR: 1.24, 95% CI: 1.15–1.34, P < 0.00001, I² = 0%, Figure 3).

FIGURE 3

Figure 3 Meta-analysis results of objective response rate (ORR) between the two groups.

Thirty-one trials with 2,358 cases reported the DCR. The meta-analysis using a random-effects model showed that Astragalus-based TCM plus PBC increased the DCR; the difference was statistically significant (RR: 1.10, 95% CI: 1.06–1.14, P < 0.00001, I² = 11%, Figure 4).

FIGURE 4

Figure 4 Meta-analysis results of disease control rate (DCR) between the two groups.

Survival Rate

Six trials (19, 26, 27, 29, 44, 52) reported the survival rate (Figure 5). Additionally, in two trials (47, 48), the survival rate was extracted from survival curves. Thus, eight trials containing 512 cases were included. The results demonstrated that there was no significant difference in the half-year survival rate between the two groups (RR: 1.14, 95% CI: 0.89–1.45, P = 0.31, I² = 90%). However, compared with the PBC-treated control group, the 1- and 2-year survival rates in the Astragalus-based TCM group were significantly improved (RR: 1.41, 95% CI: 1.09–1.82, P = 0.005, I² = 65%; RR: 3.13, 95% CI: 1.80–5.46, P = 0.84, I² = 0%).

FIGURE 5

Figure 5 Meta-analysis results of survival rate between the two groups.

Quality of Life (QOL)

In the included studies, two types of data were applied to report the QOL changes on KPS score. First, the number of patients who reported QOL improvement (KPS score 10 points higher after treatment). And second, the mean ± standard deviation (SD) of the KPS score before and after treatment. As shown in Figure 6, the number of patients with improved QOL based on the KPS score were reported in 14 studies (RR: 2.03, 95% CI: 1.70–2.43, P < 0.00001, I² = 0%); and the mean ± SD of KPS score were reported in other six studies (MD: 12.39, 95% CI: 5.48–19.30, P = 0.0004, I² = 95%) (Figure 7). Taken together, compared with PBC, the study results showed that Astragalus-containing TCM plus PBC significantly improved QOL.

FIGURE 6

Figure 6 Meta-analysis results of quality of life (QOL) according to the number of KPS improved patients.

FIGURE 7

Figure 7 Meta-analysis results of QOL according to mean ± SD.

Adverse Drug Reactions (ADRs)

Twenty-nine trials that included 2,053 patients reported ADRs principally according to the WHO criteria (12) (Table 2 and Figures S1–10). The results of the random-effects meta-analysis demonstrated that, compared with PBC alone, Astragalus-containing TCM plus PBC significantly reduced the risk of neutropenia (RR: 0.65, 95% CI: 0.54–0.79, P < 0.00001), anemia (RR: 0.68, 95% CI: 0.52–0.89, P = 0.005), thrombocytopenia (RR: 0.65, 95% CI: 0.51–0.82, P = 0.0004), nausea and vomiting (RR: 0.73, 95% CI: 0.64–0.82, P < 0.00001), diarrhea (RR: 0.58, 95% CI: 0.45–0.75, P < 0.0001), hepatic dysfunction (RR: 0.55, 95% CI: 0.40–0.77, P = 0.0005), renal dysfunction (RR: 0.48, 95% CI: 0.26–0.88, P = 0.02), neurotoxicity (RR: 0.78, 95% CI: 0.65–0.92, P = 0.004), and alopecia (RR: 0.77, 95% CI: 0.61–0.97, P = 0.03), but not the stomatitis rate (RR: 0.73, 95% CI: 0.53–1.00, P = 0.05).

TABLE 2

Table 2 Meta-analysis results of adverse drug reactions (ADRs).

Peripheral Blood Lymphocyte Levels

Ten trials that included 796 patients reported the peripheral blood lymphocyte levels. The results of CD3⁺ CD8⁺ T cell levels displayed poor clinical consistency. Therefore, we only evaluated the MD of the CD3⁺ T cells, CD3⁺ CD4⁺ T cells, the CD4⁺/CD8⁺ T cells ratio, and NK cells using a random-effects model. Our results indicated that Astragalus-containing TCM plus PBC significantly enhanced the ratio of CD3⁺ T cells (MD: 11.51, 95% CI: 5.94–17.08, P < 0.0001), CD3⁺ CD4⁺ T cells (MD: 6.44, 95% CI: 4.25–8.62, P < 0.00001), NK cells (MD: 4.58, 95% CI: 2.41–6.76, P < 0.0001) (Figure 8), and the CD4⁺/CD8⁺ T cells ratio (MD: 0.41, 95% CI: 0.27–0.55, P < 0.00001) (Figure 9) after treatment.

FIGURE 8

Figure 8 Meta-analysis results of CD3⁺ T cells, CD3⁺ CD4⁺ T cells, Nature Killer cells between the two groups.

FIGURE 9

Figure 9 Meta-analysis results of CD4⁺/CD8⁺ T cells ratio between the two groups.

Subgroup Analysis of ORR and DCR

We performed subgroup analyses in accordance with the drug delivery of Astragalus-containing TCM, PBC regimen, and treatment duration to reveal the influence of the addition of Astragalus-containing TCM on the ORR and DCR (Figures S11–16). The drug delivery of Astragalus-containing TCM was either orally or by injection. Subgroup analysis showed that Astragalus-containing TCM could improve the ORR and DCR regardless of whether it was administered orally or by injection, respectively (Table 3). PBC regimens were cisplatin-based chemotherapy and oxaliplatin-based chemotherapy regimens. Subgroup analysis displayed that Astragalus-containing TCM plus oxaliplatin-based chemotherapy could enhance both the ORR and DCR; however, when combined with cisplatin-based chemotherapy, it could only enhance the ORR (Table 3). Finally, the treatment durations were ≥8 weeks and <8 weeks. As shown in Table 3, whether the treatment duration was ≥8 weeks or <8 weeks, the combination of Astragalus-containing TCM and PBC could improve the ORR and DCR.

TABLE 3

Table 3 Subgroup analysis of ORR and DCR.

Sensitivity Analysis

We performed sensitivity analysis by omitting each study in turn to check the robustness of the main outcome, including ORR, DCR, and survival rate. This analysis showed that the pooled RR values of the ORR, DCR, and 1-year survival rate were stable, except for the half-year survival rate (Table 4). Considering that the half-year survival rate was extracted from survival curves in the study of Pan, B.Y.2018 (48), we excluded this study, and the I² decreased from 90 to 0%.

TABLE 4

Table 4 Sensitivity analysis.

As shown in Figures S17, 18, the TSA for the ORR and DCR showed that the Z-curve (blue dashed line) crossed the conventional boundary (brown dotted line), the trial sequential monitoring boundary for benefit (lower red solid line), and the RIS of the TSA (vertical red line; 660 for ORR, 574 for DCR), which suggested that the findings of the meta-analysis were robust for the ORR and DCR.

The TSA for the half-year survival rate (Figure S19) showed that the Z-curve did not cross either the conventional or trial sequential monitoring boundary, as well as the RIS (equal to 2920), which indicated that the evidence on the effect of half-year survival rate was insufficient. TSA for 1-year survival rate (Figure S20) showed that the Z-curve crossed the conventional and trial sequential monitoring boundary for benefit, but not the RIS (equal to 611). This indicates that the combination of Astragalus-containing TCM and PBC might enhance the 1-year survival rate for AGC patients. However, we did not have enough power to confirm the conclusion before RIS of 611 participants. The TSA for the 2-year survival rate (Figure S21) showed that the Z-curve crossed the conventional boundary and RIS (equal to 95), which indicated that the evidence on the effect of the 2-year survival rate was robust. Due to insufficient data, we failed to evaluate the effect on the 3-year survival rate.

Publication Bias

The funnel plots (Figures S22–33) were not strictly symmetrical in the meta-analysis of the ORR, DCR, QOL, neutropenia, anemia, thrombocytopenia, nausea and vomiting, diarrhea, hepatic dysfunction, renal dysfunction, neurotoxicity, and CD4⁺/CD8⁺ T cells ratio. However, as shown in Egger’s test (Table 5), no significant publication bias existed among the meta-analysis of ORR (P = 0.3986), QOL (P = 0.7377), diarrhea (P = 0.3092), hepatic dysfunction (P = 0.5113), renal dysfunction (P = 0.9436), and CD4⁺/CD8⁺ T cells ratio (P=0.5250).

TABLE 5

Table 5 Egger’s test.

Quality of Evidence

In summary, the quality was moderate for the ORR, 1-year survival rate, QOL, hepatic dysfunction, renal dysfunction, stomatitis, and levels of peripheral blood lymphocytes; low for DCR, neutropenia, anemia, thrombocytopenia, nausea and vomiting, diarrhea, and neurotoxicity; and very low for other results (Table 6).

TABLE 6

Table 6 GRADE evidence profile.

TABLE 6

Table 6 GRADE evidence profile.

Discussion

Gastric cancer has high incidence and morbidity levels around the world. In China, patients with AGC have poor clinical outcomes and a low 5-year survival rate of <20% (53). PBC is widely used and plays a crucial role in the treatment of AGC (54). However, the survival benefit in patients with AGC is still limited; therefore, it is necessary to develop effective combination therapies to increase the efficacy of PBC and reduce side effects in order to prolong the survival time and improve QOL in these patients.

As an important part of the comprehensive treatment of cancer, TCM, especially Astragalus-containing TCM, is used in combination with chemotherapy and broadly prescribed for AGC patients in China. To evaluate whether Astragalus-containing TCM with PBC improves the clinical efficacy and its safety, 35 RCTs involving 2,670 patients with AGC were included in this meta-analysis. To the best of our knowledge, it is the first systematic review and meta-analysis that has evaluated the efficacy and safety of Astragalus-containing TCM in combination with PBC for AGC treatment.

Our results indicated that Astragalus-containing TCM could enhance the ORR and DCR of PBC, meaning that the experimental group had a better short-term efficacy. Furthermore, we also performed subgroup analyses according to the drug delivery method of Astragalus-containing TCM, PBC regimen, and treatment duration. The results suggested that compared with PBC alone, both oral and injection-based administration of Astragalus-containing TCM resulted in better tumor response in AGC patients. In addition, both Astragalus-containing TCM combined with cisplatin-based chemotherapy and oxaliplatin-based chemotherapy resulted in a better ORR than chemotherapy alone. AGC patients that were given Astragalus-containing TCM plus oxaliplatin-based chemotherapy had better DCR; however, those who were given Astragalus-containing TCM plus cisplatin-based chemotherapy showed no difference in DCR. In addition, the subgroup analysis results also showed that Astragalus-containing TCM plus PBC resulted in better tumor response in AGC patients regardless of the duration of administration. Moreover, basic studies also showed that Astragalus and its main components could reduce or stabilize the gastric tumor by inducing antiproliferation, promoting apoptosis, and modulating the invasiveness of tumor cells, among other mechanisms (55–57). These results provided indirect basic and mechanistic evidences for the antitumor mechanisms of using Astragalus-containing TCM in AGC.

In terms of the survival rate, our study indicated that Astragalus-containing TCM plus PBC enhanced the 1- (P = 0.009) and 2-year survival rates (P < 0.0001) compared with PBC alone. However, due to the small number of included trials (n = 8), the data available for subgroup and sensitivity analyses were limited. In addition, none of the included studies completely reported the overall survival, progression-free survival, 5-year survival rate, which were important clinical survival outcomes. Therefore, conducting a meta-analysis on these outcome measures was not possible. More evidence is needed to support our findings.

PBC often leads to many adverse drug reactions in patients with AGC, which seriously impacts QOL. Therefore, reducing chemotherapy side effects while maintaining the curative effect has become a popular and urgent topic. More and more evidence has shown that Astragalus and its main components (such as Astragalus polysaccharide, flavonoid compounds, saponins compounds, alkaloids, etc.) have the potential to reduce side effects of chemotherapeutic agents (58–61). According to our results, Astragalus-containing TCM plus PBC reduced ADRs (bone marrow suppression, gastrointestinal reaction, hepatic and renal dysfunction, neurotoxicity, and alopecia) in patients with stage III/IV AGC (P < 0.05) and brought significantly improvement on their QOL based on KPS.

The human immune system plays a vital role in immune surveillance of malignant cells. Patients with AGC have low immune function, and PBC often aggravates the immunosuppressive state in patients, impairing the antitumor response (62), which increases the risk of tumor invasion and metastasis. Therefore, improving immunity is of great significance in antitumor therapy. An increasing number of pharmacological studies have shown that Astragalus and its ingredients could improve chemotherapy-induced immunosuppression, which is associated with protective effects on immune organs by regulating leukocytes, lymphocytes, and macrophages, among other cells (63–65). In clinical settings, determination of peripheral blood lymphocytes is an effective method for evaluating the immune function of patients. This meta-analysis showed that Astragalus-containing TCM plus PBC significantly increased the percentages of CD3⁺, CD3⁺CD4⁺, CD4⁺/CD8⁺, and NK cells in the peripheral blood.

This study has several limitations. Firstly, we only searched for literature from Chinese and English databases; thus, some related studies from Japanese, Korean, or other databases might have been missed. All of the RCTs included in our study were conducted in China, and only one study was published in an international journal (48); Funnel plots and egger’s test results reflected that there was some potential publication bias. Secondly, only nine studies clearly described the use of randomization methods. In most included trials, allocation concealment and blinding were “unclear”, which might result in potential implementation bias and selective bias. Thirdly, in all included trials, the follow-up duration was relatively short (≤3 years). We look forward to more longer-term follow-up studies to support our findings. Fourthly, there was substantial heterogeneity (I² > 50%) among studies for many outcomes, such as the half- and 1-year survival rate, peripheral blood lymphocyte levels, neutropenia, anemia, and thrombocytopenia, among others, which might have weakened the evidence strength of this meta-analysis. Therefore, more well-designed RCTs are needed to support our findings. Fifthly, in the treatment of AGC, Astragalus is rarely used as single-agent therapy; it usually is combined with other different herbal medicines. Additional researches are needed to further understand the specific immunological and cytotoxic mechanisms of Astragalus as an adjuvant to chemotherapy in the treatment of AGC. And lastly, based on the GRADE approach, the quality of outcomes was moderate to very low; most trials we included in our manuscript might not be reported strictly in accordance with the CONSORT reporting standards. All of these limitations might have resulted in insufficient evaluation of the outcome. However, we hope that our manuscript will make more and more doctors realize the potential benefits of Astragalus-containing TCM in AGC, and conduct more well-designed clinical trials adhering to CONSORT guidelines to verify the efficacy of Astragalus-containing TCM in the future.

Conclusion

Our study demonstrated the potencies of Astragalus-containing TCM with PBC to enhance the efficacy and safety for patients with AGC, and more efforts are needed to promote the application of Astragalus-containing TCM in the clinic. Furthermore, the long-term efficacy of Astragalus-containing TCM plus PBC in AGC treatment still needs to be verified in future well-designed clinical trials that adhere to CONSORT guidelines.

Data Availability Statement

The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.

Author Contributions

BH and YQ designed the research. MC, JH, and YZ performed literature search. YL and JJ performed article selection. RL and QG assessed methodological bias risk. XZ and YQ performed data extraction. JJ, RQ, SC, and HZ conducted a meta-analysis and assessed study quality. MC finished the manuscript draft. All authors contributed to the article and approved the submitted version.

Funding

This study was supported by the National Natural Science Foundation of China (Grant Nos. 81673961, 81774294), Beijing Municipal Science and Technology Commission (No. Z181100001618006), Beijing Natural Science Foundation (No. 7172186), Institution Subject of Guang’anmen Hospital, China Academy of Chinese Medical Sciences (Grant No. 2019S452).

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fonc.2021.632168/full#supplementary-material

Abbreviations

TCM, traditional Chinese medicine; AGC, advanced gastric cancer; PBC, platinum-based chemotherapy; RCTs, randomized controlled trials; RR, risk ratio; MD, mean difference; CI, confidence interval; TSA, trial sequential analysis; GRADE, grading of recommendations assessment, development, and evaluation; RIS, required information size; ORR, objective response rate; DCR, disease control rate; QOL, quality of life; ADRs, adverse drug reactions.

References

1. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global Cancer Statistics 2018: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin (2018) 68:394–424. doi: 10.3322/caac.21492

PubMed Abstract | CrossRef Full Text | Google Scholar

2. Eusebi LH, Telese A, Marasco G, Bazzoli F, Zagari RM. Gastric Cancer Prevention Strategies: A Global Perspective. J Gastroenterol Hepatol (2020) 35:1495–502. doi: 10.1111/jgh.15037

PubMed Abstract | CrossRef Full Text | Google Scholar

3. Hwang IG, Ji JH, Kang JH, Lee HR, Lee HY, Chi KC, et al. A Multi-Center, Open-Label, Randomized Phase III Trial of First-Line Chemotherapy With Capecitabine Monotherapy Versus Capecitabine Plus Oxaliplatin in Elderly Patients With Advanced Gastric Cancer. J Geriatr Oncol (2017) 8:170–5. doi: 10.1016/j.jgo.2017.01.002

PubMed Abstract | CrossRef Full Text | Google Scholar

4. National Comprehensive Cancer Network. (NCCN). Clinical Practice Guidelines in Oncology. Gastric Cancer, Version 2.2020. Available at: https://www.nccn.org/professionals/physician_gls/pdf/gastric.pdf (Accessed May 21, 2020).

Google Scholar

5. Li Y, Sui X, Su Z, Yu C, Shi X, Johnson NL, et al. Meta-Analysis of Paclitaxel-Based Chemotherapy Combined With Traditional Chinese Medicines for Gastric Cancer Treatment. Front Pharmacol (2020) 11:132. doi: 10.3389/fphar.2020.00132

PubMed Abstract | CrossRef Full Text | Google Scholar

6. Li J, Niu J, Yang M, Ye P, Zhai J, Yuan W, et al. Using Single-Patient (N-of-1) Trials to Determine Effectiveness of Traditional Chinese Medicine on Chemotherapy-Induced Leukopenia in Gastric Cancer: A Feasibility Study. Ann Transl Med (2019) 7:124. doi: 10.21037/atm.2019.02.03

PubMed Abstract | CrossRef Full Text | Google Scholar

7. Yao K, Ma Y, Ma W, Hu J, Wang C, Chen J, et al. Shenqifuzheng Injection Combined With Chemotherapy in the Treatment of Advanced Gastric Cancer: A Systematic Review and Meta-Analysis. J Cancer Res Ther (2014) 10:70–4. doi: 10.4103/0973-1482.139768

PubMed Abstract | CrossRef Full Text | Google Scholar

8. Lin S, An X, Guo Y, Gu J, Xie T, Wu Q, et al. Meta-Analysis of Astragalus-Containing Traditional Chinese Medicine Combined With Chemotherapy for Colorectal Cancer: Efficacy and Safety to Tumor Response. Front Oncol (2019) 9:749. doi: 10.3389/fonc.2019.00749

PubMed Abstract | CrossRef Full Text | Google Scholar

9. Cao A, He H, Wang Q, Li L, An Y, Zhou X. Evidence of Astragalus Injection Combined Platinum-Based Chemotherapy in Advanced Nonsmall Cell Lung Cancer Patients: A Systematic Review and Meta-Analysis. Med (Baltimore) (2019) 98:e14798. doi: 10.1097/MD.0000000000014798

CrossRef Full Text | Google Scholar

10. Wang SF, Wang Q, Jiao LJ, Huang YL, Garfield D, Zhang J, et al. Astragalus-Containing Traditional Chinese Medicine, With and Without Prescription Based on Syndrome Differentiation, Combined With Chemotherapy for Advanced non-Small-Cell Lung Cancer: A Systemic Review and Meta-Analysis. Curr Oncol (2016) 23:e188–95. doi: 10.3747/co.23.2920

PubMed Abstract | CrossRef Full Text | Google Scholar

11. McCulloch M, See C, Shu XJ, Broffman M, Kramer A, Fan WY, et al. Astragalus-Based Chinese Herbs and Platinum-Based Chemotherapy for Advanced non-Small-Cell Lung Cancer: Meta-Analysis of Randomized Trials. J Clin Oncol (2006) 24:419–30. doi: 10.1200/JCO.2005.03.6392

PubMed Abstract | CrossRef Full Text | Google Scholar

12. Miller AB, Hoogstraten B, Staquet M, Winkler A. Reporting Results of Cancer Treatment. Cancer (1981) 47:207–14. doi: 10.1002/1097-0142(19810101)47:1<207::aid-cncr2820470134>3.0.co;2-6

PubMed Abstract | CrossRef Full Text | Google Scholar

13. Watanabe H, Yamamoto S, Kunitoh H, Sekine I, Yamamoto N, Ohe Y, et al. Tumor Response to Chemotherapy: The Validity and Reproducibility of RECIST Guidelines in NSCLC Patients. Cancer Sci (2003) 94:1015–20. doi: 10.1111/j.1349-7006.2003.tb01394.x

PubMed Abstract | CrossRef Full Text | Google Scholar

14. Trotti A, Colevas A, Setser A, Rusch V, Jaques D, Budach V, et al. CTCAE V3.0: Development of a Comprehensive Grading System for the Adverse Effects of Cancer Treatment. Semin Radiat Oncol (2003) 13:176–81. doi: 10.1016/s1053-4296(03)00031-6

PubMed Abstract | CrossRef Full Text | Google Scholar

15. Wetterslev J, Jakobsen JC, Gluud C. Trial Sequential Analysis in Systematic Reviews With Meta-Analysis. BMC Med Res Methodol (2017) 17:39. doi: 10.1186/s12874-017-0315-7

PubMed Abstract | CrossRef Full Text | Google Scholar

16. Guyatt GH, Oxman AD, Vist GE, Kunz R, Falck-Ytter Y, Alonso-Coello P, et al. GRADE: An Emerging Consensus on Rating Quality of Evidence and Strength of Recommendations. BMJ (2008) 336:924–6. doi: 10.1136/bmj.39-489.470347.AD

PubMed Abstract | CrossRef Full Text | Google Scholar

17. Moher D, Liberati A, Tetzlaff J, Altman DG, , The PRISMA Group. Preferred Reporting Items for Systematic Reviews and Meta-Analyses: The PRISMA Statement. PLoS Med (2009) 6(7):e1000097. doi: 10.1371/journal.pmed1000097

CrossRef Full Text | Google Scholar

18. Yang J. The Clinical Investigation About Advanced Gastric Cancer With Supplementing Spleen, Retrieving Gastric Function, Expelling Dampness, Removing Stasis and Relieving Poison Associated With Oxaliplatin/5-Fu Regimen. Nanjing: Nanjing University of Chinese Medicine (2005).

Google Scholar

19. Gong LY, Jiang CM, Zhao YZ. Ai-Di Injection Combined With Chemotherapy in the Treatment for 56 Cases With Advanced Gastric Cancer. J Oncol (2006) 12:424–25. doi: 10.3969/j.issn.1671-170X.2006.05.023

CrossRef Full Text | Google Scholar

20. Chen P, Zheng WD, Gao HL, Song BJ. Clinical Analysis on the Treatment of Late Gastric Cancer by the Combination of Cidan Capsule With ELF Chemotherapy Plan. World J Integr Trad Western Med (2007) 2:167–69. doi: 10.3969/j.issn.1673-6613.2007.03.015

CrossRef Full Text | Google Scholar

21. Li AM, Li FZ. Evaluation of Therapeutic Effect of FLP Regimen Combined With Xiaoai Jiandu Decoction in the Treatment of Advanced Gastric Cancer. J Modern Med Health (2007) 23:2260. doi: 10.3969/j.issn.1009-5519.2007.15.033

CrossRef Full Text | Google Scholar

22. Wang L. The Clinical Investigation About Advanced Gastric Cancer With Supplementing Spleen, Dissipating Phlegm, Removing Stasis, Relieving Poison Associated With Chemotherapy of Paclitaxel, Oxalipatin, 5-Fu. Nanjing: Nanjing University of Chinese Medicine (2007).

Google Scholar

23. He ZQ, Cheng DY, Gong H, Cheng J, Xu ZJ, Chen D, et al. The Clinical Efficacy of FOLFOX4 Chemotherapy Combined With Delisheng in Therapy of Advanced Gastric Cancer. Modern Oncol (2008) 16:408–11. doi: 10.3969/j.issn.1672-4992.2008.03.032

CrossRef Full Text | Google Scholar

24. Liu LH, Zhang CX. Clinical Efficacy of Aidi Injection Combined With TPF in the Treatment of Advanced Gastric Carcinoma. China Mod Doct (2009) 47:16–7. doi: 10.3969/j.issn.1673-9701.2009.29.007

CrossRef Full Text | Google Scholar

25. Liu XQ. The Clinical Research of Kang’ai Injection Combined With Modified DCF Regimen for Patients With Advanced Gastric Cancer. Fujian: Fujian University of Traditional Chinese Medicine (2009).

Google Scholar

26. Zhu Y, Yuan WJ, Zhang XJ, Li X. Clinical Study of Modified Sanwu Bai San Prescription in the Treatment of Advanced Gastric Cancer. J Emergency Tradit Chin Med (2010) 19:578–80. doi: 10.3969/j.issn.1004-745X.2010.04.020

CrossRef Full Text | Google Scholar

27. Chen QS. Clinical Observation on 30 Cases of Advanced Gastric Cancer Treated With Yiqi Jianpi Decoction Combined With FOLFOX Regimen. Yunnan J Tradit Chin Med Mater Med (2011) 32:45–7. doi: 10.3969/j.issn.1007-2349.2011.04.029

CrossRef Full Text | Google Scholar

28. Du CJ, Wang TL, Guo YS. Clinical Observation of Jianpi Bushen Yiqi Yangxue Recipe in Improving the Quality of Life of Advanced Gastric Cancer. J Hebei TCM Pharmacol (2011) 26:24–5. doi: 10.3969/j.issn.1007-5615.2011.02.015

CrossRef Full Text | Google Scholar

29. Fan CM, Ge CH, Lu F, Chen Y, Xu CY, Zhu JP, et al. Therapeutic Efficacy of Aidi Injection Combined With S-1 and Oxaliplatin in Treating Patients With Advanced Gastric Cancer. Modern J Integr Tradit Chin Western Med (2011) 20:4607–9. doi: 10.3969/j.issn.1008-8849.2011.36.004

CrossRef Full Text | Google Scholar

30. Hu FS. Summary of Professor Yu Rencun’s Academic Thought and Clinical Experience and Clinical Observation of Yiqi Huoxue Jiedu Recipe Combined With Chemotherapy in the Treatment of Advanced Gastric Cancer. Beijing: Beijing University of Chinese Medicine (2011).

Google Scholar

31. Ren YZ, Wang F. Influence of Advanced Gastric Cancer Treated With Shenqi Fuzheng Injection Combined With Chemotherapy on Effect and Immune Function. J Basic Clin Oncol (2012) 25:394–96. doi: 10.3969/j.issn.1673-5412.2012.05.009

CrossRef Full Text | Google Scholar

32. Wang JR, Wang JW, Wang J, Wang ZX, Wang KM. Clinical Observation of Shengifuzheng Injection in Combination With Chemotherapy in the Treatment of Patients With Advanced Gastric Carcinoma. China Med Herald (2012) 9:79–81. doi: 10.3969/j.issn.1673-7210.2012.33.030

CrossRef Full Text | Google Scholar

33. Li HY, Ji WM, Tu J, Shen YL. Clinical Analysis of Shenqi Fuzheng Injection Combined With Chemotherapy in Patients With Advanced Gastric Cancer. China Med Herald (2013) 10:99–101. doi: 10.3969/j.issn.1673-7210.2013.15.035

CrossRef Full Text | Google Scholar

34. Tan G. Clinical Observation of Jianpi Anticancer Decoction Combined With FOLFOX Regimen in the Treatment of Advanced Gastric Cancer. Inner Mongol J Tradit Chin Med (2013) 32:75–6. doi: 10.3969/j.issn.1006-0979.2013.19.071

CrossRef Full Text | Google Scholar

35. Yin LL, Jiang CY. Observation on the Influence of Shenqi-Fuzheng Injection on T-Lymphocyte Subsets, NK Cell and the Leukocyte of the Patients With Advanced Gastric Cancer. Int J Trad Chin Med (2013) 35:22–4. doi: 10.3760/cma.j.issn.1673-4246.2013.01.007

CrossRef Full Text | Google Scholar

36. Fei YH, Wang NY, Wang Q. Clinical Study on Fuzheng Huayu Formula Combined With Chemotherapy in the Treatment of Advanced Gastric Cancer. Henan Tradit Chin Med (2014) 34:2182–183. doi: 10.16367/j.issn.1003-5028.2014.11.036

CrossRef Full Text | Google Scholar

37. Wen J. Clinical Observation of Advanced Gastric Cancer Treated With Shenqi Fuzheng Injection Combined With Chemotherapy. Yangzhou: Yangzhou University (2014).

Google Scholar

38. Xiang SL, Zhao Y. Clinical Observation of Aidi Injection Combined With TPF Regimen in the Treatment of Advanced Gastric Cancer. Med Inf (2014) 27:108. doi: 10.3969/j.issn.1006-1959.2014.37.142

CrossRef Full Text | Google Scholar

39. Zhang L. Study on Aidi Injection Combined With FOLFOX4 Chemotherapy for Advanced Gastric Cancer. Cardiovasc Dis Elec J Integr Tradit Chin Western Med (2014) 2:49–50.

Google Scholar

40. Zhang MJ, Zuo CF. Observation on the Therapeutic Effect of Aidi Injection in Patients With Advanced Gastric Cancer. Guide Chin Med (2014) 12:190–91. doi: 10.15912/j.cnki.gocm.2014.04.177

CrossRef Full Text | Google Scholar

41. Zhang YN, Zhang M, Li XF, Zhou HP. Clinical Observation on 46 Cases of Advanced Gastric Cancer Treated With Traditional Chinese Medicine for Supplementing Qi and Activating Blood Circulation. Zhejiang J Tradit Chin Med (2015) 50:656. doi: 10.13633/j.cnki.zjtcm.2015.09.023

CrossRef Full Text | Google Scholar

42. Duan F. Observation on the Efficacy of Aidi Injection Combined With Chemotherapy in the Treatment of Advanced Gastric Cancer. Chin J Clin Ration Drug Use (2016) 9:55–6. doi: 10.15887/j.cnki.13-1389/r.2016.02.034

CrossRef Full Text | Google Scholar

43. Hu Q. Observe the Clinical Curative Effect for the Treatment of Advanced Gastric Cancer With Weining Particles Combination Chemotherapy. Guangxi: Guangxi University of Traditional Medicine (2016).

Google Scholar

44. Huang P, Tao MX, Xu C, Pan YY. Clinical Effects of Jianpi Huoxue Decoction Combined With Chemotherapy on Quality of Life and Immune Function in Patients With Advanced Gastric Cancer. J Hubei Univ Chin Med (2016) 18:37–9. doi: 10.3969/j.issn.1008-987x.2016.06.11

CrossRef Full Text | Google Scholar

45. Ma M, Liu DS, Liang ES, Li XD, Cheng W, Huai XH. Clinical Observation of Fuzheng Xiaojia Recipe Combined With Chemotherapy in the Treatment of Advanced Gastric Cancer. Chin J Gerontol (2016) 36:5719–721. doi: 10.3969/j.issn.1005-9202.2016.22.105

CrossRef Full Text | Google Scholar

46. Shao KF, Xiao P. Effects of Modified Shenling Baizhu San on Immune Function and Toxicity Reaction of Advanced Gastric Cancer Patients in Chemotherapy. Guiding J Tradit Chin Med Pharm (2016) 22:26–8+32. doi: 10.13862/j.cnki.cn43-1446/r.2016.08.008

CrossRef Full Text | Google Scholar

47. Hu Q, Yang XY, Sun YS. Observation on the Therapeutic Effect of Kangai Injection Combined With Chemotherapy in Patients With Advanced Gastric Cancer. Modem Pract Med (2017) 29:886–89. doi: 10.3969/j.issn.1671-0800.2017.07.024

CrossRef Full Text | Google Scholar

48. Pan B, Zang J, He J, Wang Z, Liu L. Add-On Therapy With Chinese Herb Medicine Bo-Er-Ning Capsule (BENC) Improves Outcomes of Gastric Cancer Patients: A Randomized Clinical Trial Followed With Bioinformatics-Assisted Mechanism Study. Am J Cancer Res (2018) 8:1090–105.

PubMed Abstract | Google Scholar

49. Xu W. Observation on the Efficacy and Safety of Jianpi Jiedu Recipe in Adjuvant Chemotherapy in the Treatment of Advanced Gastric Cancer. Guangming J Chin Med (2019) 34:767–69. doi: 10.3969/j.issn.1003-8914.2019.05.047

CrossRef Full Text | Google Scholar

50. Yuan DD, Yue HG, Li LX. The Effects of Shenqifuzheng Injection on Serum miR-124 Level in Patients With Advanced Gastric Cancer. J Med Theor＆Prac (2019) 32:1799–800+812. doi: 10.19381/j.issn.1001-7585.2019.12.002

CrossRef Full Text | Google Scholar

51. Zhu YF, Zhang DW, Yu MH, Su Y, Wang CH, Xu XZ, et al. Efficacy of Aidi Injection Combined With Chemotherapy in Treatment of Advanced Gastric Cancer and Its Effects on Cancer-Related Fatigue, Quality of Life and Toxic and Side Effects. J Hubei Univ Chin Med (2019) 21:29–33. doi: 10.3969/j.issn.1008-987x.2019.04.07

CrossRef Full Text | Google Scholar

52. Hou Y, Yan B. Effects of Buzhong Yiqi Decoction Combined With XELOX Regimen on Short-Term Efficacy and Long-Term Survival Rate in Advanced Gastric Cancer. World Chin Med (2020) 15:426–29. doi: 10.3969/j.issn.1673-7202.2020.03.027

CrossRef Full Text | Google Scholar

53. Peng W, Zhang F, Wang Z, Li D, He Y, Ning Z, et al. Large Scale, Multicenter, Prospective Study of Apatinib in Advanced Gastric Cancer: A Real-World Study From China. Cancer Manag Res (2020) 12:6977–85. doi: 10.2147/CMAR.S249153

PubMed Abstract | CrossRef Full Text | Google Scholar

54. Cheng J, Cai M, Shuai X, Gao J, Wang G, Tao K. First-Line Systemic Therapy for Advanced Gastric Cancer: A Systematic Review and Network Meta-Analysis. Ther Adv Med Oncol (2019) 11:1–44. doi: 10.1177/1758835919877726

CrossRef Full Text | Google Scholar

55. Yu J, Ji H, Dong X, Feng Y, Liu A. Apoptosis of Human Gastric Carcinoma MGC-803 Cells Induced by a Novel Astragalus Membranaceus Polysaccharide via Intrinsic Mitochondrial Pathways. Int J Biol Macromol (2019) 126:811–9. doi: 10.1016/j.ijbiomac.2018.12.268

PubMed Abstract | CrossRef Full Text | Google Scholar

56. Auyeung KK, Han QB, Ko JK. Astragalus Membranaceus: A Review of its Protection Against Inflammation and Gastrointestinal Cancers. Am J Chin Med (2016) 44:1–22. doi: 10.1142/S0192415X16500014

PubMed Abstract | CrossRef Full Text | Google Scholar

57. Auyeung KK, Woo PK, Law PC, Ko JK. Astragalus Saponins Modulate Cell Invasiveness and Angiogenesis in Human Gastric Adenocarcinoma Cells. J Ethnopharmacol (2012) 141:635–41. doi: 10.1016/j.jep.2011.08.010

PubMed Abstract | CrossRef Full Text | Google Scholar

58. Ma Q, Xu Y, Tang L, Yang X, Chen Z, Wei Y, et al. Astragalus Polysaccharide Attenuates Cisplatin-Induced Acute Kidney Injury by Suppressing Oxidative Damage and Mitochondrial Dysfunction. BioMed Res Int (2020) 2020:2851349. doi: 10.1155/2020/2851349

PubMed Abstract | CrossRef Full Text | Google Scholar

59. You X, Xu Y, Huang J, Zhi Y, Wu F, Ding F, et al. A Data Mining-Based Analysis of Medication Rules in Treating Bone Marrow Suppression by Kidney-Tonifying Method. Evid Based Complement Alternat Med (2019) 2019:1907848. doi: 10.1155/2019/1907848

PubMed Abstract | CrossRef Full Text | Google Scholar

60. Yan W, Xu Y, Yuan Y, Tian L, Wang Q, Xie Y, et al. Renoprotective Mechanisms of Astragaloside IV in Cisplatin-Induced Acute Kidney Injury. Free Radic Res (2017) 51:669–83. doi: 10.1080/10715762.2017.1361532

PubMed Abstract | CrossRef Full Text | Google Scholar

61. Liu W, Gao FF, Li Q, Lv JW, Wang Y, Hu PC, et al. Protective Effect of Astragalus Polysaccharides on Liver Injury Induced by Several Different Chemotherapeutics in Mice. Asian Pac J Cancer Prev (2014) 15:10413–20. doi: 10.7314/apjcp.2014.15.23.10413

PubMed Abstract | CrossRef Full Text | Google Scholar

62. Rébé C, Ghiringhelli F. Cytotoxic Effects of Chemotherapy on Cancer and Immune Cells: How can it be Modulated to Generate Novel Therapeutic Strategies? Future Oncol (2015) 19:2645–54. doi: 10.2217/fon.15.198

CrossRef Full Text | Google Scholar

63. Li S, Sun Y, Huang J, Wang B, Gong Y, Fang Y, et al. Anti-Tumor Effects and Mechanisms of Astragalus Membranaceus (AM) and its Specific Immunopotentiation: Status and Prospect. J Ethnopharmacol (2020) 258:1–9. doi: 10.1016/j.jep.2020.112797

CrossRef Full Text | Google Scholar

64. Yang B, Xiao B, Sun T. Antitumor and Immunomodulatory Activity of Astragalus Membranaceus Polysaccharides in H22 Tumor-Bearing Mice. Int J Biol Macromol (2013) 62:287–90. doi: 10.1016/j.ijbiomac.20-13.09.016

PubMed Abstract | CrossRef Full Text | Google Scholar

65. Li J, Bao Y, Lam W, Li W, Lu F, Zhu X, et al. Immunoregulatory and Anti-Tumor Effects of Polysaccharopeptide and Astragalus Polysaccharides on Tumor-Bearing Mice. Immunopharmacol Immunotoxicol (2008) 30:771–82. doi: 10.1080/08923970802279183

PubMed Abstract | CrossRef Full Text | Google Scholar