AUTHOR=Li Hui , Wang Shuoer , Yao Qianlan , Liu Yan , Yang Jing , Xu Lun , Yang Gong 

TITLE=A Combined Long Noncoding RNA Signature as a Candidate Prognostic Biomarker for Ovarian Cancer

JOURNAL=Frontiers in Oncology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.624240

DOI=10.3389/fonc.2021.624240

ISSN=2234-943X

ABSTRACT=<sec><title>Aims</title><p>Dysregulated long noncoding RNAs (lncRNAs) contributing to ovarian cancer (OC) development may serve as prognostic biomarker. We aimed to explore a lncRNA signature to serve as prognostic biomarker of OC.</p></sec><sec><title>Methods</title><p>Univariate Cox regression was conducted on the lncRNA expression dataset from the TCGA cohort, and 246 genes significantly associated with survival were retained for building a model. A random forest survival model was carried out, and a model was developed using 6 genes with the highest frequency. The selected genes were applied in a Cox multivariate regression model for prognostic prediction by calculating the risk score. We also used CCK-8, EdU, and colony formation assays to validate the function of these lncRNAs in OC cells.</p></sec><sec><title>Results</title><p>This study confirmed that the 6-lncRNA combined signature was related to OC prognosis. Systematic analysis demonstrated that lncRNA-associated genes were enriched in oncogenic signalling pathways. Five out of the 6 lncRNAs participated in OC proliferation.</p></sec><sec><title>Conclusion</title><p>We established a 6-lncRNA combined signature for OC prognosis, which may serve as powerful prognostic biomarker for OC after further validation.</p></sec>