AUTHOR=Gao Jian , Xi Lei , Yu Rentao , Xu Huailong , Wu Min , Huang Hong 

TITLE=Differential Mutation Detection Capability Through Capture-Based Targeted Sequencing in Plasma Samples in Hepatocellular Carcinoma

JOURNAL=Frontiers in Oncology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.596789

DOI=10.3389/fonc.2021.596789

ISSN=2234-943X

ABSTRACT=<p>Circulating tumor DNA (ctDNA) is a promising biomarker for accurate monitoring and less invasive assessment of tumor burden and treatment response. Here, targeted next-generation sequencing (NGS) with a designed gene panel of 176 cancer-relevant genes was used to assess mutations in 90 ctDNA samples from 90 patients with multiple types of liver disease and 10 healthy donor samples for control. Using our ctDNA detection panel, we identified mutations in 98.89% (89/90) of patient plasma biopsy samples, and 19 coding variants located in 10 cancer-related genes [<italic>ACVR2A, PCLO, TBCK</italic>, adhesion G protein-coupled receptor (<italic>ADGRV1</italic>), <italic>COL1A1, GABBR1, MUC16, MAGEC1, FASLG</italic>, and <italic>JAK1</italic>] were identified in 96.7% of patients (87/90). The 10 top mutated genes were tumor protein p53 (<italic>TP53</italic>), <italic>ACVR2A, ADGRV1, MUC16, TBCK, PCLO, COL11A1</italic>, titin (<italic>TTN</italic>), <italic>DNAH9</italic>, and <italic>GABBR1</italic>. <italic>TTN</italic> and <italic>TP53</italic> and <italic>TTN</italic> and <italic>DNAH9</italic> mutations tended to occur together in hepatocellular carcinoma samples. Most importantly, we found that most of those variants were insertions (frameshift insertions) and deletions (frameshift deletions and in-frame deletions), such as insertion variants in <italic>ACVR2A, PCLO</italic>, and <italic>TBCK</italic>; such mutations were detected in almost 95% of patients. Our study demonstrated that the targeted NGS-based ctDNA mutation profiling was a useful tool for hepatocellular carcinoma (HCC) monitoring and could potentially be used to guide treatment decisions in HCC.</p>