AUTHOR=Du Yongjun , Hou Yanmei , Shi Yongbo , Liu Juan , Li Tingxin 

TITLE=RETRACTED: Long Non-Coding RNA ELFN1-AS1 Promoted Colon Cancer Cell Growth and Migration via the miR-191-5p/Special AT-Rich Sequence-Binding Protein 1 Axis

JOURNAL=Frontiers in Oncology

VOLUME=10

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.588360

DOI=10.3389/fonc.2020.588360

ISSN=2234-943X

ABSTRACT=<p>Long non-coding RNAs (lncRNAs) are reported to participate in tumor development. It has been manifested in previous researches that lncRNA <italic>ELFN1-AS1</italic> is involved in early-stage colon adenocarcinoma with potential diagnostic value. However, no studies have revealed the specific mechanism of <italic>ELFN1-AS1</italic> in colon cancer, and there are no other studies on whether <italic>ELFN1-AS1</italic> is associated with tumorigenesis. In our study, <italic>ELFN1-AS1</italic> with high expression in colon cancer was selected by TCGA analysis, and the survival analysis was carried out to verify it. Subsequently, qRT-PCR was adopted for validating the results in tissues and cell lines. Cell counting kit-8 (CCK8), 5-ethynyl-2’-deoxyuridine (EdU), cell colon, cell apoptosis, cell cycle, cell migration, and invasion assays were utilized to assess the role of <italic>ELFN1-AS1</italic> in colon cancer. Results uncovered that <italic>ELFN1-AS1</italic> expression was prominently raised in colon cancer cells and tissues. <italic>ELFN1-AS1</italic> decrement restrained cells to grow through interfering with distribution of cell cycle and promoting apoptosis. Meanwhile, <italic>ELFN1-AS1</italic> decrement weakened the capacity of cells to migrate and invade. What’s more, <italic>ELFN1-AS1</italic> was uncovered to act as a competing endogenous RNA (ceRNA) to decrease miR-191-5p expression, thus raising <italic>special AT-rich sequence-binding protein 1</italic> (<italic>SATB1</italic>), a downstream target of ceRNA. To sum up, <italic>ELFN1-AS1</italic> drives colon cancer cells to proliferate and invade through adjusting the miR-191-5p/<italic>SATB1</italic> axis. The above results disclose that lncRNA <italic>ELFN1-AS1</italic> is possibly a novel treatment target for colon cancer cases.</p>