AUTHOR=Zheng Yuehuan , Chen Zhe , Zhou Zezhu , Xu Xiangyang , Yang Huilin 

TITLE=Silencing of Long Non-Coding RNA LINC00607 Prevents Tumor Proliferation of Osteosarcoma by Acting as a Sponge of miR-607 to Downregulate E2F6

JOURNAL=Frontiers in Oncology

VOLUME=10

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.584452

DOI=10.3389/fonc.2020.584452

ISSN=2234-943X

ABSTRACT=<p>Osteosarcoma (OS), a type of malignant bone tumor, is commonly found in children and adolescents. Although previous studies have identified that long non-coding RNAs (lncRNAs) regulate OS, it is unclear whether lncRNAs impact the progression of OS. Here, we identified <italic>LINC00607</italic>, a lncRNA that facilitates OS proliferation, migration, and invasion. Based on the RNA-sequencing results, <italic>LINC00607</italic> expression was significantly upregulated in pulmonary metastasis within OS. Functional experiments revealed that <italic>LINC00607</italic> promoted migration and invasion of endothelial cells to exacerbate epithelial-mesenchymal transition (EMT). Furthermore, the results of RNA pull-down assay and invasion assay suggested that the binding between <italic>LINC00607</italic> and miR-607 promoted OS invasion. Bioinformatic analysis and rescue experiments demonstrated that <italic>E2F6</italic>, a transcriptional factor, functioned downstream of <italic>LINC00607</italic>/miR-607. Finally, we found that <italic>LINC00607</italic> promoted OS progression <italic>in vivo</italic>. This work revealed that <italic>LINC00607</italic> worked as an miR-607 sponge to upregulate <italic>E2F6</italic> expression, which promoted tumor proliferation in OS. These results identified a novel therapeutic target for treating OS.</p>