AUTHOR=Huang Wei , Lu Jian , Tang Rongbiao , Wu Zhiyuan , Wang Qingbing , Ding Xiaoyi , Wang Zhongmin , Chen Kemin 

TITLE=Phase Contrast Imaging Based Microbubble Monitoring of Radiofrequency Ablation: An ex vivo Study

JOURNAL=Frontiers in Oncology

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.01709

DOI=10.3389/fonc.2020.01709

ISSN=2234-943X

ABSTRACT=<sec><title>Background</title><p>To explore the potential of synchrotron radiation (SR) phase contrast imaging (PCI) for real-time microbubble formation monitoring during radiofrequency ablation (RFA).</p></sec><sec><title>Methods</title><p>RFA was performed on <italic>ex vivo</italic> porcine muscle tissue using unipolar and multi-tined expandable electrodes. Images of microbubble formation in the samples were captured by both SR PCI and absorption contrast imaging. The synchronous ablation temperature was recorded. Each RFA electrode type group contained 6 samples. Ablation size was assessed by histologic examination.</p></sec><sec><title>Results</title><p>Microbubble formation during RFA could be visualized by SR PCI. The diameter of the microbubbles revealed on the image ranged from tens of microns to several millimeters, and these microbubbles first appeared at the edge of the RFA electrode when the target region temperature reached approximately 60°C and rapidly extended outwards. The average microbubble range measured on PCI was 17.66 ± 0.74 mm. The average range of coagulation necrosis measured by histological examination was 17.22 ± 0.38 mm. There was no significant difference between them (<italic>P</italic> &gt; 0.05). The range of microbubbles corresponded to the ablation zone.</p></sec><sec><title>Conclusion</title><p>PCI enabled real-time high-resolution visualization of microbubble formation during RFA, indicating a potential for its use in ablation monitoring.</p></sec>