AUTHOR=Zahnreich Sebastian , Poplawski Alicia , Hartel Carola , Eckhard Lukas Stefan , Galetzka Danuta , Hankeln Thomas , Löbrich Markus , Marron Manuela , Mirsch Johanna , Ritter Sylvia , Scholz-Kreisel Peter , Spix Claudia , Schmidberger Heinz TITLE=Spontaneous and Radiation-Induced Chromosome Aberrations in Primary Fibroblasts of Patients With Pediatric First and Second Neoplasms JOURNAL=Frontiers in Oncology VOLUME=10 YEAR=2020 URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.01338 DOI=10.3389/fonc.2020.01338 ISSN=2234-943X ABSTRACT=
The purpose of the present study was to investigate whether former childhood cancer patients who developed a subsequent secondary primary neoplasm (SPN) are characterized by elevated spontaneous chromosomal instability or cellular and chromosomal radiation sensitivity as surrogate markers of compromised DNA repair compared to childhood cancer patients with a first primary neoplasm (FPN) only or tumor-free controls. Primary skin fibroblasts were obtained in a nested case-control study including 23 patients with a pediatric FPN, 22 matched patients with a pediatric FPN and an SPN, and 22 matched tumor-free donors. Clonogenic cell survival and cytogenetic aberrations in Giemsa-stained first metaphases were assessed after X-irradiation in G1 or on prematurely condensed chromosomes of cells irradiated and analyzed in G2. Fluorescence