AUTHOR=Paradiso Angelo Virgilio , Patruno Margherita , Digennaro Maria , Tommasi Stefania , Pilato Brunella , Argentiero Antonella , Brunetti Oronzo , Silvestris Nicola TITLE=Somatic BRCA Mutation in a Cholangiocarcinoma Patient for HBOC Syndrome Detection JOURNAL=Frontiers in Oncology VOLUME=10 YEAR=2020 URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.01292 DOI=10.3389/fonc.2020.01292 ISSN=2234-943X ABSTRACT=

BRCA-associated hereditary breast and ovarian cancer syndrome (HBOC) is characterized by an increased risk of developing other malignancies including cholangiocarcinoma (CCA). Somatic BRCA mutations have been reported in CCA, but they have yet to be utilized in a proband case to identify HBOC in families. Two healthy daughters of a deceased female patient who had had metachronous breast cancer and CCA received genetic counseling to assess their cancer risk. Somatic BRCA1/2 mutation analysis was performed by next-generation sequencing on the DNA extracted from a formalin-fixed, paraffin-embedded CCA biopsy specimen of their mother. A pathogenic variant was identified (c.6468_6469delTC in a BRCA2 gene mutation). Germline BRCA mutation analysis of the two daughters detected the same pathogenic variant in one of them. For the first time, a CCA somatic BRCA mutation has been used to identify a family with HBOC.