AUTHOR=Xu Zhihua , Gao Guanzhuang , Liu Fei , Han Ye , Dai Chen , Wang Sentai , Wei Guobang , Kuang Yuting , Wan Daiwei , Zhi Qiaoming , Xu Ye 

TITLE=Molecular Screening for Nigericin Treatment in Pancreatic Cancer by High-Throughput RNA Sequencing

JOURNAL=Frontiers in Oncology

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.01282

DOI=10.3389/fonc.2020.01282

ISSN=2234-943X

ABSTRACT=<p><bold>Objectives:</bold> Nigericin, an antibiotic derived from <italic>Streptomyces hygroscopicus</italic>, has been proved to exhibit promising anti-cancer effects on a variety of cancers. Our previous study investigated the potential anti-cancer properties in pancreatic cancer (PC), and demonstrated that nigericin could inhibit the cell viabilities in concentration- and time-dependent manners via differentially expressed circular RNAs (circRNAs). However, the knowledge of nigericin associated with long non-coding RNA (lncRNA) and mRNA in pancreatic cancer (PC) has not been studied. This study is to elucidate the underlying mechanism from the perspective of lncRNA and mRNA.</p><p><bold>Methods:</bold> The continuously varying molecules (lncRNAs and mRNAs) were comprehensively screened by high-throughput RNA sequencing.</p><p><bold>Results:</bold> Our data showed that 76 lncRNAs and 172 mRNAs were common differentially expressed in the nigericin anti-cancer process. Subsequently, the bioinformatics analyses, including Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, coding and non-coding co-expression network, cis- and trans-regulation predictions and protein-protein interaction (PPI) network, were applied to annotate the potential regulatory mechanisms among these coding and non-coding RNAs during the nigericin anti-cancer process.</p><p><bold>Conclusions:</bold> These findings provided new insight into the molecular mechanism of nigericin toward cancer cells, and suggested a possible clinical application in PC.</p>