AUTHOR=Racanelli Dominga , Brenca Monica , Baldazzi Davide , Goeman Frauke , Casini Beatrice , De Angelis Biagio , Guercio Marika , Milano Giuseppe Maria , Tamborini Elena , Busico Adele , Dagrada Gianpaolo , Garofalo Cecilia , Caruso Chiara , Brunello Antonella , Pignochino Ymera , Berrino Enrico , Grignani Giovanni , Scotlandi Katia , Parra Alessandro , Hattinger Claudia Maria , Ibrahim Toni , Mercatali Laura , De Vita Alessandro , Carriero Maria Vincenza , Pallocca Matteo , Loria Rossella , Covello Renato , Sbaraglia Marta , Dei Tos Angelo Paolo , Falcioni Rita , Maestro Roberta TITLE=Next-Generation Sequencing Approaches for the Identification of Pathognomonic Fusion Transcripts in Sarcomas: The Experience of the Italian ACC Sarcoma Working Group JOURNAL=Frontiers in Oncology VOLUME=10 YEAR=2020 URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.00489 DOI=10.3389/fonc.2020.00489 ISSN=2234-943X ABSTRACT=

This work describes the set-up of a shared platform among the laboratories of the Alleanza Contro il Cancro (ACC) Italian Research Network for the identification of fusion transcripts in sarcomas by using Next Generation Sequencing (NGS). Different NGS approaches, including anchored multiplex PCR and hybrid capture-based panels, were employed to profile a large set of sarcomas of different histotypes. The analysis confirmed the reliability of NGS RNA-based approaches in detecting sarcoma-specific rearrangements. Overall, the anchored multiplex PCR assay proved to be a fast and easy-to-analyze approach for routine diagnostics laboratories.