AUTHOR=Wang Ye , Mashock Michael , Tong Zhuang , Mu Xiaofeng , Chen Hong , Zhou Xin , Zhang Hong , Zhao Gexin , Liu Bin , Li Xinmin 

TITLE=Changing Technologies of RNA Sequencing and Their Applications in Clinical Oncology

JOURNAL=Frontiers in Oncology

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.00447

DOI=10.3389/fonc.2020.00447

ISSN=2234-943X

ABSTRACT=<p>RNA sequencing (RNAseq) is one of the most commonly used techniques in life sciences, and has been widely used in cancer research, drug development, and cancer diagnosis and prognosis. Driven by various biological and technical questions, the techniques of RNAseq have progressed rapidly from bulk RNAseq, laser-captured micro-dissected RNAseq, and single-cell RNAseq to digital spatial RNA profiling, spatial transcriptomics, and direct <italic>in situ</italic> sequencing. These different technologies have their unique strengths, weaknesses, and suitable applications in the field of clinical oncology. To guide cancer researchers to select the most appropriate RNAseq technique for their biological questions, we will discuss each of these technologies, technical features, and clinical applications in cancer. We will help cancer researchers to understand the key differences of these RNAseq technologies and their optimal applications.</p>