AUTHOR=Qi Lu , Song Fuyao , Ding Yanqing 

TITLE=Regulatory Mechanism of ITGBL1 in the Metastasis of Colorectal Cancer

JOURNAL=Frontiers in Oncology

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.00259

DOI=10.3389/fonc.2020.00259

ISSN=2234-943X

ABSTRACT=<p>Integrin, beta-like 1 (ITGBL1) protein is located in the extracellular matrix (ECM) and involved in the development and metastasis of many tumors. However, the regulatory mechanism of ITGBL1 in colorectal cancer (CRC) remains unclear. This study was to analyze the expression profile of CRC and to identify the expression change of <italic>ITGBL1</italic> gene at different stages of CRC. Survival analysis showed that <italic>ITGBL1</italic> was related to the metastasis of CRC, and CRC patients with a high expression of <italic>ITGBL1</italic> had earlier metastasis. Gene Set Enrichment Analysis (GSEA) indicated the relationship between <italic>ITGBL1</italic> expression and molecular events of CRC. The results indicated that a high expression of <italic>ITGBL1</italic> was linked to Wnt signaling pathway, cell polarity, and tissue development, while a low expression of <italic>ITGBL1</italic> was related to cellular respiration, electron transfer chain, and oxidative phosphorylation. With the expression profiles from interstitial and parenchyma CRC tissues, a comparison was made to determine the difference between high/low expression of <italic>ITGBL1</italic> and Wnt signaling pathway, respectively, and further confirmed the close relation between <italic>ITGBL1</italic> and Wnt signaling pathway. To determine the relation, an interaction network of ITGBL1 and Wnt signaling proteins was constructed. It was found that β-catenin interacted with multiple extracellular Wnt signals and could bind to ITGBL1. As a result, the regulatory mechanism of ITGBL1 in CRC is related to extracellular Wnt signals and may affect extracellular Wnt signals via β-catenin.</p>