AUTHOR=Zhao Huan , Zheng Chunlei , Wang Yizhe , Hou Kezuo , Yang Xianghong , Cheng Yang , Che Xiaofang , Xie Shilin , Wang Shuo , Zhang Tieqiong , Kang Jian , Liu Yunpeng , Pan Dianzhu , Qu Xiujuan , Hu Xuejun , Fan Yibo 

TITLE=miR-1323 Promotes Cell Migration in Lung Adenocarcinoma by Targeting Cbl-b and Is an Early Prognostic Biomarker

JOURNAL=Frontiers in Oncology

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.00181

DOI=10.3389/fonc.2020.00181

ISSN=2234-943X

ABSTRACT=<p><bold>Purpose:</bold> MicroRNAs are known to regulate cellular processes in non-small cell lung cancer (NSCLC) cells and predict prognosis. However, identification of specific microRNAs in NSCLC as potential therapeutic targets is controversial. We aim to determine the clinical significance of miR-1323 in the prognosis of patients with lung cancer and the potential mechanism.</p><p><bold>Patients and methods:</bold> A bioinformatics approach was used to screen the importance microRNA in NSCLC through the online GEO database (GSE42425). The relationship between expression level of miR-1323 and overall survival of lung cancer patients was analyzed. Additionally, an independent corhort including 53 NSCLC cases that underwent resection validated the connection between miR-1323 and LUAD patients' overall survival. Next, the function of miR-1323 was studied <italic>in vitro</italic> by transient transfection. A more in-depth mechanism was studied through luciferase reporter gene experiments.</p><p><bold>Results:</bold> High miR-1323 expression correlated with poor survival in NSCLC patients (<italic>P</italic> = 0.011), and in lung adenocarcinoma (LUAD) patients (<italic>P</italic> = 0.015) based on GEO database (GSE42425). In the independent cohort based on our hospital, high miR-1323 expression was associated with LUAD patients (<italic>P</italic> = 0.025). Moreover, transfection with mimics of miR-1323 showed an increased migratory capacity in LUAD A549 and HCC827 cells. In addition, E3 ubiquitin-protein ligase Casitas B-lineage Lymphoma-b (Cbl-b) was found to be the target genes of miR-1323 and significantly down regulated after mimics of miR-1323 transfection, and high Cbl-b expression predicted better prognosis in NSCLC and LUAD (<italic>P</italic> = 0.00072 and <italic>P</italic> = 0.02, respectively).</p><p><bold>Conclusion:</bold> The miR-1323 promoted LUAD migration through inhibiting Cbl-b expression. High miR-1323 expression predicted poor prognosis in LUAD patients.</p>