AUTHOR=Sangiorgi Bruno , de Souza Felipe Canto , Mota de Souza Lima Ildercílio , dos Santos Schiavinato Josiane Lilian , Corveloni Amanda Cristina , Thomé Carolina Hassibe , Araújo Silva Wilson , Faça Vitor Marcel , Covas Dimas Tadeu , Zago Marco Antônio , Panepucci Rodrigo Alexandre TITLE=A High-Content Screening Approach to Identify MicroRNAs Against Head and Neck Cancer Cell Survival and EMT in an Inflammatory Microenvironment JOURNAL=Frontiers in Oncology VOLUME=9 YEAR=2019 URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2019.01100 DOI=10.3389/fonc.2019.01100 ISSN=2234-943X ABSTRACT=
Head and neck squamous cell carcinoma (HNSCC) is among the most common cancer types. Metastasis, the main cause of death by cancer, can be promoted by an inflammatory microenvironment, which induces epithelial-mesenchymal transition (EMT) through a NF-κB-mediated stabilization of Snail. Here, we aimed to explore how microRNAs (miRs) can affect cell survival and EMT in HNSCC cells under an inflammatory microenvironment. By using a high-content screening (HCS) approach, we evaluated alterations in morphometric parameters, as well as expression/localization of Snail/Slug, in HNSCC cells primed with TNF-α. Based on those quantitation, we established the optimal experimental conditions of EMT induction driven by TNF-α. Those conditions were applied to cells transfected with distinct miRs (