AUTHOR=Ye Jin , Zou Man-Man , Li Pei , Lin Xi-Jun , Jiang Qi-Wei , Yang Yang , Huang Jia-Rong , Yuan Meng-Ling , Xing Zi-Hao , Wei Meng-Ning , Li Yao , Shi Zhi , Liu Hui 

TITLE=Oxymatrine and Cisplatin Synergistically Enhance Anti-tumor Immunity of CD8+ T Cells in Non-small Cell Lung Cancer

JOURNAL=Frontiers in Oncology

VOLUME=8

YEAR=2018

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2018.00631

DOI=10.3389/fonc.2018.00631

ISSN=2234-943X

ABSTRACT=<p>Oxymatrine (OMT) has shown broad antitumor activities for the treatment of several types of cancers. However, little is known about its effect on anti-tumor immunity. Combination therapy is a potentially promising strategy of cancer to enhance anticancer activity, overcome drug resistance, and lower treatment failure rate. In the present study, we demonstrated that the combination of OMT with cisplatin (DDP) synergistically inhibited non-small cell lung cancer (NSCLC) cells growth when co-cultured with peripheral blood mononuclear cells <italic>in vitro</italic>. Furthermore, the combination of OMT with DDP significantly inhibited the growth of Lewis lung cancer (LLC) mouse xenograft tumors. Flow cytometry analysis revealed that OMT and DDP synergistically increase the CD8<sup>+</sup>/ regulatory T cells ratio and enhanced more CD8<sup>+</sup> T cells secreted cytokines of IFN-γ, TNF-α, and IL-2 <italic>in vivo</italic>. Mechanistically, upregulation of <italic>miR-155</italic> and downregulation of suppressor of cytokine signaling-1 (<italic>SOCS1</italic>) were confirmed as a target signaling pathway to positively regulate the anti-tumor response of CD8<sup>+</sup> T cells. Overall, OMT in combination with DDP showed outstanding synergistic anti-tumor immunity, suggesting that this beneficial combination may offer a potential immunotherapy for NSCLC patients.</p>