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REVIEW article

Front. Oncol., 03 February 2012
Sec. Molecular and Cellular Oncology
This article is part of the Research Topic The key role of ubiquitination and sumoylation in signaling and cancer View all 11 articles

The role of Elongin BC-containing ubiquitin ligases


      Fumihiko Okumura* Fumihiko Okumura*Mariko Matsuzaki Mariko MatsuzakiKunio Nakatsukasa and Takumi Kamura* Kunio Nakatsukasa and Takumi Kamura*
  • Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Aichi, Japan

The Elongin complex was originally identified as a positive regulator of RNA polymerase II and is composed of a transcriptionally active subunit (A) and two regulatory subunits (B and C). The Elongin BC complex enhances the transcriptional activity of Elongin A. “Classical” SOCS box-containing proteins interact with the Elongin BC complex and have ubiquitin ligase activity. They also interact with the scaffold protein Cullin (Cul) and the RING domain protein Rbx and thereby are members of the Cullin RING ligase (CRL) superfamily. The Elongin BC complex acts as an adaptor connecting Cul and SOCS box proteins. Recently, it was demonstrated that classical SOCS box proteins can be further divided into two groups, Cul2- and Cul5-type proteins. The classical SOCS box-containing protein pVHL is now classified as a Cul2-type protein. The Elongin BC complex containing CRL family is now considered two distinct protein assemblies, which play an important role in regulating a variety of cellular processes such as tumorigenesis, signal transduction, cell motility, and differentiation.

Introduction

Polyubiquitin-mediated protein degradation plays an important role in the elimination of short-lived regulatory proteins (Peters, 1998), including those that contribute to the cell cycle, cellular signaling in response to environmental stress or extracellular ligands, morphogenesis, secretion, DNA repair, and organelle biogenesis (Hershko and Ciechanover, 1998). The system responsible for the attachment of ubiquitin to the target protein consists of several components that act in concert (Hershko and Ciechanover, 1992; Scheffner et al., 1995), including a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin–protein isopeptide ligase (E3). E3 is believed to be the component of the ubiquitin conjugation system that is most directly responsible for substrate recognition (Scheffner et al., 1995). Based on structural similarity, E3 enzymes have been classified into three families: the HECT (homologous to E6-AP COOH terminus) family (Huibregtse et al., 1995; Hershko and Ciechanover, 1998), the RING finger-containing protein family (Lorick et al., 1999; Freemont, 2000; Joazeiro and Weissman, 2000), and the U box family (Aravind and Koonin, 2000; Hatakeyama et al., 2001; Cyr et al., 2002). The S phase kinase-associated protein 1 (Skp1)–Cullin 1 (Cul1)–F box protein (SCF) family is a member of the RING finger-containing ubiquitin ligase family (Lipkowitz and Weissman, 2011). Cul1 is a scaffold protein and assembles multiple proteins into complexes, which include a small RING finger protein (Rbx1), an adaptor protein (Skp1), and a substrate-targeting protein (F box protein). Substrate recognition by the RING finger-containing ubiquitin ligase family is modulated by post-translational modifications of the target substrate, including phosphorylation, glycosylation, and sumoylation (Lipkowitz and Weissman, 2011). One substrate can be polyubiquitinated by different ubiquitin ligases and vice versa. The Elongin B and C–Cul2 or Cul5–SOCS box protein (ECS) family also belongs to the Cullin RING ligase (CRL) superfamily (Kile et al., 2002). SCF and ECS ubiquitin ligases have structural similarities in that both contain Rbx1 or Rbx2 as a RING finger protein and Cul1, Cul2, or Cul5 as a scaffold protein (Kile et al., 2002; Kamura et al., 2004). Although Skp1 is used as an adaptor protein in the SCF complex, the Elongin B and C complex is used as an adaptor in the ECS complex. Here, we review the Cul2- or Cul5-containing ECS ubiquitin ligase family, about which, compared to SCF ubiquitin ligases, relatively little is known.

The Elongin Complex

The Elongin complex is a positive regulator of RNA polymerase II (pol II) and increases the rate of elongation by suppressing transient pausing along the DNA template (Bradsher et al., 1993a,b). The Elongin complex is composed of a transcriptionally active A subunit and two regulatory subunits, B and C (Garrett et al., 1994, 1995; Aso et al., 1995). Elongin B and C form the Elongin BC complex, which enhances the transcriptional activity of Elongin A. Since Elongin B and C partially resemble ubiquitin and Skp1 (an adaptor of SCF-type ubiquitin ligases), respectively (Bai et al., 1996), they are able to serve as components of protein complexes with functions other than transcriptional regulation. For example, they were found to be components of the von Hippel–Lindau (VHL) tumor suppressor complex, which is also known as the ECS complex (Figure 1; Duan et al., 1995; Kibel et al., 1995).

FIGURE 1
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Figure 1. Comparison of the structures of SCF- and ECS-type ubiquitin ligases. (A) SCF-type ubiquitin ligases. Cul1 is used as a scaffold protein and Skp1 bridges the gap between the F box protein and Cul1. (B) Cul2-type ubiquitin ligases. Cul2 is used as a scaffold protein and the Elongin BC complex connects the VHL box protein to Cul2. The Cul2 box determines the use of Cul2 as the scaffold protein. (C) Cul5-type ubiquitin ligases. Cul5 is used as a scaffold protein. Note that the Rbx2 is used for the recruitment of E2 enzyme.

Cul2-Type Ubiquitin Ligase

CRL2pVHL Complex

von Hippel–Lindau disease is a hereditary cancer syndrome caused by germline mutations in the VHL tumor suppressor gene (Latif et al., 1993). pVHL is the protein product of the VHL tumor suppressor gene and can bind to the Elongin BC complex. Elongin A and pVHL share a conserved Elongin C-binding sequence motif (S,T,P)LXXX(C,S,A)XXXΦ, which is referred to as the BC box (Conaway et al., 1998; Mahrour et al., 2008). More than 70% of VHL disease and sporadic clear cell renal carcinomas are caused by mutation or deletion of the BC box, which reduces binding affinity to the Elongin BC complex (Duan et al., 1995; Kishida et al., 1995). Approximately 57% of sporadic clear cell renal carcinomas contain inactivating mutations of VHL, of which 98% are caused by loss of heterozygosity (LOH) at the VHL locus (Gnarra et al., 1994). Epigenetic silencing of VHL by DNA methylation is also involved in the inactivation of VHL (Herman et al., 1996). Although pVHL inhibits the transcriptional activity of Elongin A by competing for binding sites on the Elongin BC complex (Duan et al., 1995), this review will focus on its ubiquitin ligase activity rather than its affect on Elongin-mediated transcription. In addition to the Elongin BC complex, the VHL complex also contains Cul2 and Rbx1 and is similar to SCF (Skp1–Cul1–F box protein) type ubiquitin ligases (Figure 1; Kibel et al., 1995; Pause et al., 1997; Kamura et al., 1999). In fact, the VHL complex has ubiquitin ligase activity and targets the hypoxia-inducible factor-α (HIF-α) family of transcription factors (HIF-1–3α) for proteasomal degradation (Figure 2; Maxwell et al., 1999). At normal oxygen levels, proline residues of the LXXLAP sequence motif within the oxygen-dependent degradation domain (ODDD) of HIF-α are hydroxylated and recognized by pVHL (Ivan et al., 2001; Jaakkola et al., 2001; Masson et al., 2001; Hon et al., 2002). As a result, HIF-α is polyubiquitinated and degraded. Three HIF prolyl hydroxylases (PHD1–3) have been identified in mammals and shown to hydroxylate HIF-α subunits (Epstein et al., 2001). Since PHD2 is a critical enzyme for the hydroxylation of HIF-1α, PHD1, and 3 may hydroxylate other target substrates (Berra et al., 2003). In low oxygen conditions, PHDs are unable to hydroxylate the HIF-α subunits, which are therefore not recognized and targeted for degradation by pVHL. The unhydroxylated HIF-α dimerizes with constitutively expressed HIF-1β, also called aryl hydrocarbon receptor nuclear translocator (ARNT), and translocates to the nucleus, where it induces the transcription of downstream target genes, including vascular endothelial growth factor A (VEGFA), solute carrier family 2 member 1 (SLC2A1, which encodes GLUT1), and platelet-derived growth factor-β (PDGFB; Kourembanas et al., 1990; Wizigmann-Voos et al., 1995; Gnarra et al., 1996; Iliopoulos et al., 1996; Maxwell et al., 1999). Loss of functional pVHL protein prevents the O2-dependent degradation of HIF-α, resulting in constitutive expression of HIF-dependent genes and consequently VHL disease.

FIGURE 2
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Figure 2. Regulation of HIF-α protein by pVHL. In normoxia, two proline residues of HIF-α are hydroxylated by PHD1–3, and then HIF-α is recognized by pVHL for polyubiquitination and degradation by the proteasome. On the other hand, in hypoxia, HIF-α escapes prolyl hydroxylation and thereby escapes degradation. HIF-α then dimerizes with HIF-1β to form an active transcription complex. Prolonged activation of the HIFα/β transcription complex is a major contributor to VHL disease.

The pVHL protein contains the recently defined “VHL box,” which is composed of a BC box and a Cul2 box (Figure 3; Kamura et al., 2004). The Cul2 box specifically recognizes the endogenous Cul2/Rbx1 complex in a similar manner to SCF-type ubiquitin ligase recognition by F box and Skp1 (Figure 1; Kamura et al., 2004). The ring finger protein Rbx1 recruits ubiquitin-conjugating enzymes and is an essential molecule for the formation of SCF-type and Cul2-type ubiquitin ligases (Kamura et al., 1999, 2004; Ohta et al., 1999; Seol et al., 1999; Skowyra et al., 1999; Tan et al., 1999). Further study demonstrated that the Cul2 box is located 8–23 amino acids C-terminal to the BC box and has the consensus sequence ΦPXXΦXXXΦ, where the first position is most frequently a leucine (Mahrour et al., 2008). The Cul2 box is therefore partially similar to the Cul5 box.

FIGURE 3
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Figure 3. Domain organization of VHL box proteins. (A) The VHL box consists of a BC box and a Cul2 box in the order indicated. LRR, leucine-rich repeats; SWIMZ, SWI2/SNF2 MuDR zinc fingers. (B) Alignment of amino acid sequences of selected Cul2 boxes. Identical amino acids are highlighted in yellow. GenBank™ accession numbers of each protein are indicated. The consensus sequence is indicated below. Φ, hydrophobic residue.

pVHL also polyubiquitinates and induces the degradation of Sprouty2 (Spry2), which is implicated in the growth and progression of tumors (Anderson et al., 2011). Proline residues of Spry2 are hydroxylated by PHD at normoxia and are recognized by pVHL for polyubiquitination and degradation (Anderson et al., 2011). Epidermal growth factor receptor (EGFR) is also targeted by pVHL for polyubiquitination and degradation (Zhou and Yang, 2011). pVHL is proposed to down-regulate tumor growth caused by prolonged signaling of activated EGFR (Zhou and Yang, 2011). pVHL also mediates the polyubiquitination of the atypical PKCs, PKCλ, and PKCζII (Okuda et al., 2001; Iturrioz and Parker, 2007). PKCζII interacts with Par6, which plays a critical role in the development of tight junction structures and apico-basal polarization. It also inhibits tight junction formation and thereby plays a regulatory role in the development and transformation of cell polarity (Suzuki et al., 2001; Parkinson et al., 2004). PKCλ may have a similar function, which is also inhibited by pVHL through ubiquitin-dependent degradation. pVHL also polyubiquitinates the seventh subunit of human RNA polymerase II (hsRPB7) and suppresses hsRPB7-dependent VEGF promoter transactivation, VEGF mRNA expression, and VEGF protein secretion (Na et al., 2003). The large subunit of RNA polymerase II (Rpb1), which has sequence and structural similarity to the pVHL-binding domain of HIF-1α, is bound and polyubiquitinated by pVHL (Figure 4; Kuznetsova et al., 2003). The interaction between pVHL and Rpb1 is enhanced by hyperphosphorylation of Rpb1 by UV radiation, which indicates that Rpb1 ubiquitination may have a role in transcription-coupled DNA repair (Figure 4; Svejstrup, 2002; Kuznetsova et al., 2003). Further study showed that proline 1465 of Rpb1, which is located within the LXXLAP motif, is hydroxylated mainly by PHD1 during oxidative stress (Mikhaylova et al., 2008). pVHL is necessary for the oxidative stress-dependent hydroxylation of Pro1465, the phosphorylation of Ser5, and the polyubiquitination of Rpb1 and its recruitment to the DNA (Mikhaylova et al., 2008). Surprisingly, in renal clear cell carcinoma (RCC), pVHL increased the protein abundance and non-degradative ubiquitination of Rpb1 (Mikhaylova et al., 2008). Polyubiquitination of Rpb1 in RCC cells by pVHL contributes to tumor growth by modulating gene expression (Mikhaylova et al., 2008). This is different from previous results found in PC12 cells, in which pVHL polyubiquitinates Rpb1 for protein degradation (Kuznetsova et al., 2003). How pVHL differentially regulates Rpb1 in cells of different origins awaits further investigation.

FIGURE 4
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Figure 4. Regulation of the large subunit of RNA polymerase II (Rpb1) by pVHL and Elongin A. Ser5 and Pro1465 of Rpb1 are phosphorylated and hydroxylated, respectively, by oxidative stress and then recognized by the Cul2-type ubiquitin ligase pVHL. The resulting polyubiquitinated Rpb1 is not degraded by proteasome and contributes to tumor growth by modulating gene expression in renal clear cell carcinoma. Ser5 of Rpb1 is also phosphorylated by UV irradiation and recognized by the Cul5-type ubiquitin ligase Elongin A for proteasomal degradation.

CRL2LRR-1 Complex

Leucine-rich repeat protein (LRR)-1 contains a VHL box and physiologically interacts with the endogenous Cul2–Rbx1 complex (Figure 3; Kamura et al., 2004; Costessi et al., 2011). In fact, nematode LRR-1 degrades the Cip/Kip CDK-inhibitor CKI-1 in C. elegans to promote cell cycle progression in germ cells (Starostina et al., 2010). Human LRR-1 also polyubiquitinates the CDK-inhibitor p21Cip1; however, it does not affect cell cycle progression (Starostina et al., 2010). Rather, human LRR-1 targets cytoplasmic p21 for degradation to prevent the inhibition of the Rho/ROCK/LIMK pathway (Starostina et al., 2010). These data indicate that human LRR-1 is a negative regulator of cofilin, an actin-depolymerizing protein that decreases cell motility (Starostina et al., 2010).

CRL2FEM1B Complex

Feminization-1 (FEM-1) also contains a VHL box and physiologically interacts with endogenous Cul2–Rbx1 complex (Figure 3). FEM-1 regulates apoptosis during the sex determination pathway of the nematode (Hodgkin et al., 1985). In C. elegans, FEM-1 interacts with CED-4, an Apaf-1 homolog, to promote apoptosis, suggesting an evolutionarily conserved role in apoptosis regulation (Chan et al., 2000). Nematode FEM-1 polyubiquitinates TRA-1, a Gli-family transcription factor and terminal effector of the sex determination pathway (Starostina et al., 2007). Mouse FEM-1 homolog B (FEM1B) interacts with and polyubiquitinates ankyrin repeat domain 37 (Ankrd37), which contains ankyrin repeats and a putative nuclear localization signal (NLS; Shi et al., 2011). Ankrd37 is highly enriched in mouse testis and is conserved from zebrafish to humans (Shi et al., 2011). These data indicate that the terminal step in sex determination is controlled by ubiquitin-mediated proteolysis.

Feminization-1 is polyubiquitinated by SEL-10, an F box and WD40 repeat protein, for proteasomal degradation (Jager et al., 2004). In mammalian cells, receptor for activated C kinase (RACK)1, also a WD40 repeat protein, associates with FEM1B and mediates the polyubiquitination and downregulation of FEM1B (Subauste et al., 2009). RACK1 also binds to the Elongin BC complex and promotes the ubiquitination of HIF-1α independently of the pVHL complex (Liu et al., 2007). Since the Elongin BC binding site in RACK1 is similar to that of pVHL, it has been suggested that RACK1 is a Cul2-type ubiquitin ligase (Liu et al., 2007).

CRL2PRAME Complex

Preferentially expressed antigen of melanoma (PRAME) contains a VHL box and physiologically interacts with endogenous Cul2–Rbx1 complex (Kamura et al., 2004; Costessi et al., 2011). Genome-wide chromatin immunoprecipitation experiments revealed that PRAME is specifically enriched at enhancers and at transcriptionally active promoters that are also bound by nuclear transcription factor Y (NFY), a transcription factor essential for early embryonic development (Bhattacharya et al., 2003; Costessi et al., 2011). However, the physiological substrates of PRAME have not yet been identified.

Cul5-Type Ubiquitin Ligase

CRL5Cis/SOCS Complex

This family consists of suppressor of cytokine signaling (SOCS) proteins and cytokine-inducible Src homology 2 (SH2) domain-containing protein (CIS, also known as CISH), which also interacts with the Elongin BC complex through its SOCS box (Piessevaux et al., 2008). To date, eight CIS/SOCS family proteins have been identified: CIS, SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, and SOCS7. All of them have a central SH2 domain as well as a C-terminally located SOCS box consisting of a 40-amino acid motif (Figure 5; Endo et al., 1997; Naka et al., 1997; Starr et al., 1997). Members of the CIS/SOCS family bind to janus kinases (JAKs), certain cytokine receptors, or signaling molecules, thereby suppressing downstream signaling events (Piessevaux et al., 2008). A small kinase inhibitory region (KIR) of SOCS1 and SOCS3 inhibits the JAKs by acting as a pseudo-substrate, resulting in the downregulation of further signal transduction (Piessevaux et al., 2008). The CIS/SOCS family can also down-regulate signaling by competing with downstream molecules for binding to the activated receptors (Ram and Waxman, 1999; Piessevaux et al., 2008) and can prevent signaling by polyubiquitination and degradation of target substrates. For example, SOCS1 polyubiquitinates JAK2, Vav, IRS1, and IRS2 (De Sepulveda et al., 2000; Kamizono et al., 2001; Rui et al., 2002). Recent studies also demonstrated that SOCS1 and SOCS3 are important regulators of adaptive immunity (Kile et al., 2002; Tamiya et al., 2011). Some SOCS box-containing proteins – for example, CIS, SOCS1–7, SPRY domain-containing SOCS box proteins (SSB1, SSB2, and SSB4, also known as SPSB1, 2, and 4, respectively), ras-related protein Rab-40C (also known as RAR3), WD40 repeat-containing SOCS box protein WSB1, leucine-rich repeat protein MUF1, and ankyrin repeat- and SOCS box-containing protein (ASB)11 – also contain a BC box and a Cul5 box inside the SOCS box (Figure 5; Hilton et al., 1998; Kamura et al., 2001, 2004; Babon et al., 2009; Sartori da Silva et al., 2010). The amino acid sequence LPΦP in the Cul5 box results in a specific interaction with Cul5, particularly when there is a proline in the fourth position of the motif (Kamura et al., 2004). Furthermore, endogenous Cul5 interacts with endogenous Rbx2, enabling SOCS box-containing proteins to form a protein complex with Cul5 and Rbx2 (Figure 1C; Kamura et al., 1999, 2004; Ohta et al., 1999). The selective interactions between Cul2 and Rbx1 or Cul5 and Rbx2 suggest that Rbx1 and Rbx2 are functionally distinct, at least in terms of their specific binding to Cullin family members. Although SOCS1 contains a Cul5 box, no interaction between SOCS1 and Cul5 has been detected, most likely because the Cul5 box is incompletely conserved (Kamura et al., 2004). Since SOCS1 polyubiquitinates JAK2, Vav, IRS1, and IRS2 (De Sepulveda et al., 2000; Kamizono et al., 2001; Rui et al., 2002), it is possible that the interaction of SOCS1 with these substrates recruits other ubiquitin ligase(s) that actually mediate their polyubiquitination and degradation, or that SOCS1 binds to the Cul5–Rbx2 module too weakly to have been previously detected (Kamura et al., 2004). Recently, it was reported that SOCS1 and SOCS3 bind more weakly to Cul5, with affinities of 100- and 10-fold lower, respectively, than to the rest of the family (Babon et al., 2009). In general, micromolar affinities are common in physiological interactions, and SOCS1 and SOCS3 have 1 and 0.1 μM affinities, respectively, for Cul5 (Babon et al., 2009). Therefore it is possible that all CIS/SOCS family members can act as ubiquitin ligases. This may explain why only SOCS1 and SOCS3 have been shown to suppress signaling using both SOCS box-dependent and -independent mechanisms (Babon et al., 2009).

FIGURE 5
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Figure 5. Domain organization of SOCS box proteins. (A) The SOCS box consists of a BC box and a Cul5 box in the order indicated. SH2, Src homology 2 phosphotyrosine binding domain; WD40, WD40 repeats; SPRY, sp1A/ryanodine receptor domain; Ank, ankyrin repeats; LRR, leucine-rich repeats; GTPase, GTPase domain. (B) Alignment of amino acid sequences of selected Cul5 boxes. Identical amino acids are highlighted in yellow. GenBank™ accession numbers of each protein are indicated. The consensus sequence is indicated below. Φ, hydrophobic residue.

CRL5Elongin A Complex

pVHL is a ubiquitin ligase for the large subunit of RNA polymerase II (Rpb1), as mentioned above. Interestingly, ubiquitination and proteasomal degradation of Rpb1 following UV irradiation are significantly suppressed in Elongin A-deficient cells, which suggests that Elongin A is also a ubiquitin ligase for Rpb1 (Yasukawa et al., 2008). In fact, polyubiquitination and degradation are rescued by the transfection of wild-type Elongin A (Yasukawa et al., 2008). Furthermore, Elongin A and the Elongin BC complex can associate with Cul5 and Rbx2, and this complex efficiently polyubiquitinates Rpb1 in vitro (Yasukawa et al., 2008). Phosphorylation of Rpb1 at Ser5 after UV irradiation significantly enhanced the interaction between Elongin A and Rpb1 (Yasukawa et al., 2008). These data indicate that Elongin A, like pVHL, is involved in the ubiquitination and degradation of Rpb1 following DNA damage (Figure 4).

CRL5SSB Complex

Inducible nitric oxide (NO) synthase (iNOS, NOS2) is a high-output NOS compared with NOS1 and NOS3. The activity of iNOS is approximately 10-fold greater than that of NOS1 and NOS3 (Lowenstein and Padalko, 2004). iNOS is not expressed under normal conditions but is induced in response to cytokines, microbes, or microbial products, resulting in the sustained production of NO (Lowenstein and Padalko, 2004). As a result, reactive nitrogen intermediates (such as NO, nitrite, and nitrate) and the products of the interaction of NO with reactive oxygen species (such as peroxynitrite and peroxynitrous acid) are accumulated and used to inhibit viruses or bacteria (Fang, 1997; Nathan and Shiloh, 2000; Lowenstein and Padalko, 2004). SSB1, 2 and 4 polyubiquitinate iNOS for proteasomal degradation (Kuang et al., 2010; Nishiya et al., 2011). SSB2-deficient macrophages showed prolonged iNOS and NO production, resulting in the enhanced killing of L. major parasites (Kuang et al., 2010). Further study showed that SSB1 and SSB4 are major ubiquitin ligases for iNOS and prevent the overproduction of NO, which could cause cytotoxicity (Nishiya et al., 2011).

CRL5WSB1 Complex

WSB1 polyubiquitinates homeodomain-interacting protein kinase 2 (HIPK2), which is a nuclear protein kinase and is well-conserved from Drosophila to humans (Choi et al., 2005, 2008). HIPK2 interacts with a variety of transcription factors (D’Orazi et al., 2002; Hofmann et al., 2002; Zhang et al., 2005; Kim et al., 2006), the p300/CBP co-activator (Kim et al., 2002; Aikawa et al., 2006), and the Groucho/TLE co-repressor (Choi et al., 2005). The loss of HIPK2 reduces apoptosis and increases the numbers of trigeminal ganglia, while the overexpression of HIPK2 in the developing sensory and sympathetic neurons promotes apoptosis in a caspase-dependent manner (Doxakis et al., 2004; Wiggins et al., 2004). HIPK2 plays an important role in apoptosis mediated by p53, CtBP, Axin, Brn3, Sp100, TP53INP1, and PML (Moller et al., 2003a,b; Tomasini et al., 2003; Doxakis et al., 2004; Kanei-Ishii et al., 2004). UV irradiation activates and stabilizes HIPK2, most likely by WSB1-independent auto-phosphorylation, which results in the phosphorylation of p53 at Ser46. Expression of p53 target genes then promotes apoptosis (D’Orazi et al., 2002; Hofmann et al., 2002). Genotoxic stresses, such as adriamycin and cisplatin, also inhibit polyubiquitination of HIPK2 by WSB1 (Choi et al., 2008). HIPK2 also phosphorylates CtBP at Ser422 and phosphorylated CtBP is degraded via the 26S proteasome, resulting in apoptosis in p53-deficient cells (Zhang et al., 2003). WSB1 expression is induced by Sonic hedgehog (Shh) in developing limb buds and other embryonic structures (Vasiliauskas et al., 1999). WSB1 also ubiquitinates the thyroid hormone-activating enzyme type 2 iodothyronine deiodinase (D2; Dentice et al., 2005). Ubiquitination of Shh-induced D2 by WSB1 induces parathyroid hormone-related peptide (PTHrP), thereby regulating chondrocyte differentiation (Dentice et al., 2005). In addition to HIPK2 and D2, WSB1 also binds to the interleukin-21 receptor (IL-21R; Nara et al., 2011). However, instead of promoting its degradation, WSB1 inhibits the degradation of the mature form of IL-21R (Nara et al., 2011). WSB1 associates with the intracytoplasmic region of IL-21R and enhances the maturation of IL-21R from an N-linked glycosylated form to a fully glycosylated mature form (Nara et al., 2011). These data indicate that WSB1 has important roles in both the maturation and the degradation of IL-21R.

CRL5ASB Complex

ASB2, 3, 4, 6, 9, and 11 can all bind to Cul5–Rbx2 and form ubiquitin ligase complexes. Retinoic acid induces ASB2 in acute promyelocytic leukemia cells (Guibal et al., 2002). ASB2 targets the actin-binding proteins filamin A and B for proteasomal degradation (Heuze et al., 2008). Since knockdown of endogenous ASB2 in leukemia cells delays retinoic acid-induced differentiation and filamin degradation, ASB2 may regulate hematopoietic cell differentiation by targeting filamins for degradation and thereby modulating actin remodeling (Heuze et al., 2008). ASB2 and Skp2 associate with each other to bridge the formation of a non-canonical cullin1- and cullin5-containing dimeric ubiquitin ligase complex and promote the polyubiquitination and degradation of Jak3 (Nie et al., 2011; Wu and Sun, 2011).

Tumor necrosis factor receptor type 2 (TNF-R2) is polyubiquitinated by ASB3 for proteasomal degradation (Chung et al., 2005). ASB3 can affect T cell signaling by degrading TNF-R2, resulting in the inhibition of downstream signaling events in response to TNF-α (Chung et al., 2005).

Insulin receptor substrate 4 (IRS4) is an adaptor molecule involved in signal transduction by both insulin and leptin, and is widely expressed throughout the hypothalamus, with the greatest expression observed in the medial preoptic nucleus, ventromedial hypothalamus, and arcuate nucleus (Numan and Russell, 1999). ASB4 co-localizes and interacts with IRS4 in hypothalamic neurons (Li et al., 2011). ASB4 polyubiquitinates IRS4 for degradation and decreases insulin signaling (Li et al., 2011).

ASB6 is expressed in 3T3-L1 adipocytes but not in fibroblasts (Wilcox et al., 2004). ASB6 may regulate components of the insulin signaling pathway in adipocytes by promoting the degradation of adapter protein with a pleckstrin homology and SH2 domain (APS; Wilcox et al., 2004).

ASB9 polyubiquitinates creatine kinase B (CKB) and decreases total CKB levels (Debrincat et al., 2007).

The notch signaling pathway is essential for the spatio-temporal regulation of cell fate (Mumm and Kopan, 2000; Lai, 2004; Louvi and Artavanis-Tsakonas, 2006). The single-pass transmembrane protein delta acts as a ligand for the notch receptor. Danio rerio Asb11 (d-Asb11) regulates compartment size in the endodermal and neuronal lineages via the ubiquitination and degradation of deltaA, leading to the activation of the canonical notch pathway (Diks et al., 2006, 2008). This recognition is specific to deltaA because d-Asb11 does not degrade deltaD (Diks et al., 2008). In zebrafish embryos, knockdown of d-Asb11 repressed specific delta–notch elements and their transcriptional targets, whereas these were induced when d-Asb11 was misexpressed (Diks et al., 2008). These data indicate that d-Asb11 regulates delta–notch signaling for the fine-tuning of lateral inhibition gradients between deltaA and notch (Diks et al., 2008).

CRL5Rab-40C Complex and CRLMUF1 Complex

The substrates of Rab-40C and MUF1 have not yet been identified. However, Rab-40C localizes in the perinuclear recycling compartment, suggesting its physiological role in receptor endocytosis (Rodriguez-Gabin et al., 2004). Given that the mRNA and protein level of Rab-40C increases as oligodendrocytes differentiate, it may be important in myelin formation (Rodriguez-Gabin et al., 2004).

Viral ECS-Type Ubiquitin Ligase

CRL2HPV16E7 Complex

Human papillomavirus (HPV) type 16 cause premalignant squamous intraepithelial neoplasia (Munger et al., 2004). Integration of viral DNA into the host genome leads to persistent and dysregulated expression of HPV E6 and E7 oncoproteins, which is necessary for the induction and maintenance of the oncogenic transformation (Munger et al., 2004). HPV E7 contains incomplete Cul2 box and can bind to endogenous Cul2 (Huh et al., 2007). HPV E7 polyubiquitinates retinoblastoma tumor suppressor (pRB) and induces proteasomal degradation (Boyer et al., 1996; Berezutskaya et al., 1997; Jones and Munger, 1997; Huh et al., 2007).

CRL5Vif Complex

The viral infectivity factor (Vif) protein of human immunodeficiency virus-1 (HIV-1) is also a Cul5-type ubiquitin ligase (Yu et al., 2003; Bergeron et al., 2010). Importantly, it has been suggested that the zinc-binding motif of Vif is important for its interaction with Cul5 (Yu et al., 2004; Mehle et al., 2006; Xiao et al., 2006). Vif polyubiquitinates and degrades the cellular intrinsic restriction factors APOBEC3F and APOBEC3G (Yu et al., 2003; Mehle et al., 2004; Liu et al., 2005). Both APOBEC3F and G have cytidine deaminase activity and, when packaged into HIV-1 virions, cause uracil (U) to be substituted for cytosine (C) in newly synthesized minus-strand viral DNA (Sheehy et al., 2002; Harris et al., 2003; Lecossier et al., 2003; Mangeat et al., 2003; Mariani et al., 2003). The C-to-U mutation introduced into minus-strand viral DNA results in a guanine (G)-to-adenine (A) mutation in plus-strand viral DNA because U is read as T by DNA polymerases (Lecossier et al., 2003). These mutations cause amino acid substitutions, which affect the enzymatic activity of HIV-1 (Harris et al., 2003). Another possibility is that deoxyuridine in minus-strand viral DNA is targeted for excision by uracil-DNA glycosylase (Harris et al., 2003). These abasic sites are recognized and cleaved by endonucleases, inhibiting HIV-1 replication (Harris et al., 2003). Since the CRL5Vif complex targets APOBEC3F and APOBEC3G for proteasomal degradation, it is a potential target for the development of antiviral agents aimed at preventing the interaction between Vif and Cul5.

CRL5E4orf6 Complex

The human adenovirus type 5 (Ad5) early region 4 34-kDa product from open reading frame 6 (E4orf6) contains three BC boxes (Blanchette et al., 2004; Cheng et al., 2007, 2011). Although Ad5 E4orf6 forms complex containing Cul5, Elongin BC complex, and Rbx1, Cul5 box is not present in the Ad5 E4orf6 (Harada et al., 2002; Blanchette et al., 2004; Cheng et al., 2011). Adenoviral protein E1B55K associates with the E4orf6 protein and recognizes substrate to be degraded by ubiquitin–proteasome pathway (Blanchette et al., 2004; Cheng et al., 2007; Luo et al., 2007). This complex is essential for efficient viral replication and some substrates have been identified, including p53 (Moore et al., 1996; Querido et al., 1997; Steegenga et al., 1998; Cathomen and Weitzman, 2000; Nevels et al., 2000; Shen et al., 2001), meiotic recombination 11 (Mre11; Stracker et al., 2002; Blanchette et al., 2004), DNA ligase IV (Baker et al., 2007), integrin α3 (Dallaire et al., 2009), and adeno-associated virus type 5 (AAV5) Rep52 and capsid proteins (Nayak et al., 2008). The Mre11 complex consists of Mre11, RAD50, and Nijmegen breakage syndrome 1 (NBS1, also known as nibrin) is a sensor of DNA double-strand breaks (DSBs) and induces p53-dependent apoptosis (Stracker and Petrini, 2011). DNA ligase IV plays a pivotal role in repairing DSBs and the mutation of this gene results in ligase IV (LIG4) syndrome characterized by pronounced radiosensitivity, genome instability, malignancy, immunodeficiency, and bone marrow abnormalities (Chistiakov et al., 2009). Heterodimer of integrin α and β subunits functions as transmembrane receptor that links external ligands to intracellular signaling pathways. Integrin α3β1 heterodimer in which the α3 subunit is coupled to the β1 subunit binds a variety of extracellular matrix substrates, including fibronectin, collagen, vitronectin, and laminins (DiPersio et al., 1995). E4orf6/E1B55K ligase complex is involved in cell detachment from the extracellular matrix, which may contribute to virus spread (Dallaire et al., 2009). Although Cul5 is present in the E4orf6 complex of the human Ad5, Cul2 is primarily present in the E4orf6 complex of Ad12 and Ad40 (Cheng et al., 2011). Interestingly, E4orf6 complex of Ad16 binds Cul2 as well as Cul5 and is not able to degrade p53 and integrin α3 (Cheng et al., 2011). It remains unclear how E4orf6 complexes of each serotypes distinguish Cul2 from Cul5.

CRL5BZLF1 Complex

Epstein–Barr virus (EBV), a human γ-herpesvirus, is associated with several B cell and epithelial cell malignancies and there are two different infection states, latent, and lytic (Tsurumi, 2001). BZLF1 (known as Zta, EB1, or ZEBRA), is a transcriptional trans-activator that induces EBV early gene expression to activate an EBV lytic cycle cascade (Chevallier-Greco et al., 1986; Countryman et al., 1987; Hammerschmidt and Sugden, 1988; Sinclair et al., 1991). BZLF1 can bind to Cul2 and Cul5 because of presence of both Cul2 and Cul5 boxes (Sato et al., 2009a). BZLF1 polyubiquitinates and induces degradation of p53 (Sato et al., 2009a,b). The degradation of p53 prevents apoptosis and is required for the efficient viral propagation in the lytic replication.

Conclusion

The “classical” SOCS box proteins can be divided into two distinct families. Cul2 and Cul5 within the VHL box and SOCS box, respectively, determine the association with Rbx1 or Rbx2. Given that Rbx1 and Rbx2 specifically interact with Cul2 and Cul5, respectively, the functions of Rbx1 and Rbx2 are different from each other, at least in higher eukaryotes. Cul2- and Cul5-type ubiquitin ligases are structurally similar because they have the Elongin BC complex adaptor protein and Cullin scaffold protein in common. As with other ubiquitin ligases, these two have various substrates and physiological functions (Tables 1, 2, and 3) and may have arisen independently during evolution.

TABLE 1
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Table 1. Cul2-type ubiquitin ligases and corresponding substrates.

TABLE 2
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Table 2. Cul5-type ubiquitin ligases and corresponding substrates.

TABLE 3
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Table 3. Viral ECS-type ubiquitin ligases and corresponding substrates.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

This work was supported in part by a Grant-in-Aid from Scientific Research on Innovative Areas and grants from the Ministry of Education, Science, Sports, and Culture of Japan.

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Keywords: ubiquitin, Cullin, Elongin, ECS complex, SCF complex

Citation: Okumura F, Matsuzaki M, Nakatsukasa K and Kamura T (2012) The role of Elongin BC-containing ubiquitin ligases. Front. Oncol. 2:10. doi: 10.3389/fonc.2012.00010

Received: 30 November 2011; Paper pending published: 12 December 2011;
Accepted: 17 January 2012; Published online: 03 February 2012.

Edited by:

Wenyi Wei, Harvard Medical School, USA

Reviewed by:

Qing Zhang, Dana Farber Cancer Institute, USA
Qin Yan, Yale University, USA
Haifeng Yang, Lerner Research Institute, USA

Copyright: © 2012 Okumura, Matsuzaki, Nakatsukasa and Kamura. This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

*Correspondence: Fumihiko Okumura and Takumi Kamura, Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, Japan. e-mail: okumura.fumihiko@a.mbox.nagoya-u.ac.jp; z47617a@nucc.cc.nagoya-u.ac.jp

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