AUTHOR=Betton Blandine , Gannon Philippe O., Koumakpayi Ismaël Hervé , Diallo Jean-Simon , Mes-Masson Anne-Marie , Saad Fred 

TITLE=Influence of pH on the Cytotoxic Activity of Inositol Hexakisphosphate (IP6) in Prostate Cancer

JOURNAL=Frontiers in Oncology

VOLUME=1

YEAR=2011

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2011.00040

DOI=10.3389/fonc.2011.00040

ISSN=2234-943X

ABSTRACT=<p><bold>Objectives:</bold> In the present study, we investigated whether the pH of IP6 could influence its anti-tumoral activity <italic>in vitro</italic>. <bold>Methods:</bold> PC-3 cells were exposed to IP6 at pH 5, pH 7, and pH 12 and we evaluated the metabolic activity (WST-1 assay), cell proliferation (cell count), cell cycle distribution (FACS), and mitochondrial depolarization (JC-1 staining) <italic>in vitro</italic>. <bold>Results:</bold> Our results demonstrated that IP6 at pH 5 and pH 12 were more potent at lowering the metabolic activity of PC-3 cells than IP6 at pH 7. Treatment with IP6 at pH 12 also caused the greatest inhibition in cellular proliferation and accumulation of PC-3 cells in sub-G1. Finally, IP6 at pH 12 lead to a reduction in phospho-AKT and phospho-PDK1 and upregulated phospho-ERK. <bold>Conclusion:</bold> Together, our data strongly suggest that the pH of IP6 effectively modulates its anti-tumoral activity and should be reported in future studies.</p>