AUTHOR=Fiecke Chelsey , Crimmins Meghan , Hameed Ahsan , Sims Clark , Williams D. Keith , Bode Lars , Martinez Audrey , Andres Aline , Ferruzzi Mario G. 

TITLE=Dietary modulation of human milk bioactives is associated with maternal FUT2 secretor phenotype: an exploratory analysis of carotenoids and polyphenol metabolites

JOURNAL=Frontiers in Nutrition

VOLUME=11

YEAR=2024

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2024.1463969

DOI=10.3389/fnut.2024.1463969

ISSN=2296-861X

ABSTRACT=<sec id="sec1"><title>Introduction</title><p>Maternal diet modifies profiles of human milk oligosaccharides (HMOs), carotenoids, and polyphenols in human milk (HM). However, substantial variability in profiles exists between women, highlighting the complexity of non-dietary factors modulating these profiles. The objective of this study was to carry out a secondary analysis exploring the effect of maternal diet on HM carotenoids and polyphenols and relationships between dietary modulation of HM bioactives (carotenoids, polyphenols, and oligosaccharides) and maternal α1,2-fucosyltransferase 2 (FUT2) secretor phenotype.</p></sec><sec id="sec2"><title>Methods</title><p>In this pilot study, 16 exclusively breastfeeding women with obesity were enrolled between 4 and 5 months postpartum. The women were provided a 4-week meal plan consistent with the 2020 Dietary Guidelines for Americans (DGA). HM was collected for 24 h at baseline and post-intervention. Maternal FUT2 secretor phenotype was determined by 2′-fucosyllactose concentration in HM (non-secretor: &lt; 100 nmol/ml; secretor: ≥100 nmol/ml). Concentrations of carotenoids and HMOs were determined by LC and polyphenol metabolites by UPLC–MS/MS.</p></sec><sec id="sec3"><title>Results</title><p>Thirteen women completed the study (6 secretors, 7 non-secretors). The change in HM concentrations of the HMOs lacto-N-tetraose (LNT, <italic>p</italic> = 0.007), lacto-N-fucopentaose II (LNFP II, <italic>p</italic> = 0.02), difucosyllacto-N-tetraose (DFLNT, <italic>p</italic> = 0.003), and disialyllacto-N-tetraose (DSLNT, <italic>p</italic> = 0.003) and polyphenol metabolites 4-hydroxybenzoic acid (4-HBA, <italic>p</italic> = 0.08) and ferulic acid (<italic>p</italic> = 0.02) over the intervention time frame was differentially associated with maternal secretor status. 4-HBA and ferulic acid positively correlated with HMOs LNT and DSLNT (<italic>r<sub>rm</sub></italic> = 0.82–0.90, <italic>p</italic> = 0.03–0.06) for secretors but not for non-secretors. Only secretors demonstrated a negative correlation between 4-HBA and DFLNT (<italic>r<sub>rm</sub></italic> = −0.94, <italic>p</italic> = 0.001).</p></sec><sec id="sec4"><title>Discussion</title><p>The influence of maternal diet on composition of HMOs and polyphenol metabolites in HM differs based on maternal secretor status. Consideration of non-dietary factors is needed to evaluate differences in response of HM bioactives to dietary modulation.</p></sec>