AUTHOR=Ji Yun-Jeong , Kang Min Hye , Kim Geum-Soog , Kim Hyung Don , Jang Gwi Yeong 

TITLE=Platycodon grandiflorum exhibits anti-neuroinflammatory potential against beta-amyloid-induced toxicity in microglia cells

JOURNAL=Frontiers in Nutrition

VOLUME=11

YEAR=2024

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2024.1427121

DOI=10.3389/fnut.2024.1427121

ISSN=2296-861X

ABSTRACT=<sec id="sec1"><title>Background/objectives</title><p><italic>Platycodon grandiflorum</italic> (PG) is used in traditional oriental medicine to treat several ailments.</p></sec><sec id="sec2"><title>Methods</title><p>The study investigated the anti-inflammatory and neuroprotective effects of PGW (<italic>P. grandiflorum</italic>) extract in Aβ25-35-induced inflammation in BV2 microglia cells.</p></sec><sec id="sec3"><title>Result</title><p>PGW demonstrated significant inhibition of nitric oxide (NO) production, with reductions of 30.4, 36.7, and 61.2% at concentrations of 50, 100, and 200 μg/mL, respectively. Moreover, PGW effectively suppressed the production of pro-inflammatory cytokines IL-1β and IL-6 and exhibited significant inhibitory activity against TNF-α at 200 μg/mL. Furthermore, PGW treatment mitigated apoptosis in Aβ-induced BV2 cells by modulating the mitochondrial apoptosis pathway, regulating Bcl-2 family protein synthesis, and inhibiting caspase activation. Mechanistically, PGW attenuated the activation of the MAPK (JNK, ERK, p38) pathway induced by Aβ, showing a concentration-dependent decrease in phosphorylation levels of these proteins. Additionally, PGW inhibited the NF-κB pathway activation by reducing the phosphorylation levels of p65 and IκBα in a concentration-dependent manner.</p></sec><sec id="sec4"><title>Conclusion</title><p>PGW demonstrated anti-inflammatory and neuroprotective effects in Aβ-induced neuronal cells, suggesting its potential as a therapeutic agent for neuroinflammatory associated with neurodegenerative diseases.</p></sec>