AUTHOR=Li Zhe , Zhang Yiwen , Tan Xiaozhi , Kian Deng Tye , Gao Qian , Xiao Xiaomin , Xu Chengfang 

TITLE=Origin of the neonatal gut microbiota and probiotic intervention: a randomized controlled trial

JOURNAL=Frontiers in Nutrition

VOLUME=11

YEAR=2024

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2024.1389417

DOI=10.3389/fnut.2024.1389417

ISSN=2296-861X

ABSTRACT=<sec id="sec1"><title>Objective</title><p>This study aims to evaluate the origin of the neonatal gut microbiota on the 14th day and probiotic intervention in the third trimester.</p></sec><sec id="sec2"><title>Methods</title><p>Samples were obtained from a total of 30 pregnant individuals and their offspring, divided into a control group with no intervention and a probiotic group with live combined <italic>Bifidobacterium</italic> and <italic>Lactobacillus</italic> tablets, analyzing by 16S rRNA amplicon sequencing of the V4 region to evaluate the composition of them. Non-metric multidimensional scaling and SourceTracker were used to evaluate the origin of neonatal gut microbiota.</p></sec><sec id="sec3"><title>Results</title><p>We found that the microbiota in the neonatal gut at different times correlated with that in the maternal microbiota. The placenta had more influence on meconium microbiota. Maternal gut had more influence on neonatal gut microbiota on the 3rd day and 14th day. We also found that the maternal gut, vaginal, and placenta microbiota at full term in the probiotic group did not have a significantly different abundance of <italic>Bifidobacterium</italic>, <italic>Lactobacillus,</italic> or <italic>Streptococcus</italic>. However, some other bacteria changed in the maternal gut and their neonatal gut in the probiotic group.</p></sec>