AUTHOR=Su Peiwen , Qiu Honghao , Liang Lishan , Weng Luo , Liu Yingjie , Liu Jiajun , Wu Liyan , Meng Fanxin 

TITLE=The antioxidant activity of polysaccharides from Armillaria gallica

JOURNAL=Frontiers in Nutrition

VOLUME=11

YEAR=2024

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2024.1277877

DOI=10.3389/fnut.2024.1277877

ISSN=2296-861X

ABSTRACT=<p>The purpose of this study was to investigate the antioxidant activity of <italic>Armillaria gallica</italic> polysaccharides. It explored whether <italic>Armillaria gallica</italic> polysaccharides (AgP) could prevent HepG<sub>2</sub> cells from H<sub>2</sub>O<sub>2</sub>-induced oxidative damage. The results demonstrated that HepG<sub>2</sub> cells were significantly protected by AgP, and efficiently suppressed the production of reactive oxygen species (ROS) in HepG<sub>2</sub> cells. Additionally, AgP significantly decreased the abnormal leakage of alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) caused by H<sub>2</sub>O<sub>2</sub>, protecting cell membrane integrity. It was discovered that AgP was also found to regulate the activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX), while reducing malondialdehyde (MDA), thus protecting cells from oxidative damage. According to the flow cytometry analysis and measurement of caspase-3, caspase-8, and caspase-9 activities, AgP could modulate apoptosis-related proteins and attenuate ROS-mediated cell apoptosis.</p>