AUTHOR=Yan Su , Liu Xianbin , Wang Yuwen , Yang Xiaomin , Bai Lu , Sun Lin , Zhou Yifa , Cui Sisi 

TITLE=Structural characterization and antioxidant activity of pectic polysaccharides from Veronica peregrina L.

JOURNAL=Frontiers in Nutrition

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2023.1217862

DOI=10.3389/fnut.2023.1217862

ISSN=2296-861X

ABSTRACT=<sec><title>Background</title><p>Pectins are a class of acidic polysaccharides with complex structures. Different pectin molecules are composed of different domains, which have an important impact on their biological activity.</p></sec><sec><title>Objective</title><p>This study aimed to determine the structural features and the antioxidant activities of the pectic polysaccharides isolated from <italic>Veronica peregrina</italic>  L.</p></sec><sec><title>Methods</title><p>The polysaccharide was isolated from <italic>Veronica peregrina</italic> L by water extraction and fractionated by ion exchange chromatography and gel permeation chromatography. The structure features of the pectic polysaccharides were determined by Fourier transforminfrared spectroscopy (FT-IR) and Nuclear magnetic resonance (NMR). The antioxidant activities was evaluated by the DPPH, OH and ABTS radical scavenging ability.</p></sec><sec><title>Results</title><p>WVPP-A2b and WVPP-A3b, with molecular weights of 48.7 × 10<sup>4</sup> and 77.6 × 10<sup>4</sup>  kDa, respectively, contained homogalacturonan (HG), rhamnogalacturonan I (RG-I), and rhamnogalacturonan II (RG-II) domains with a mass ratio of 2.08:2.64:1.00 and 3.87:4.65:1:00, respectively. The RG-I domain contained an arabinogalactan II backbone and arabinans consisting of t-Ara<italic>f</italic>, (1→5)-α-Ara<italic>f</italic>, and (1→3,5)-α-Ara<italic>f</italic>. WVPP-A3b also contained short chains consisting of the [t-Ara<italic>f</italic>-(1→5)-α-Ara<italic>f</italic>-(1→] structural unit. WVPP-A3b showed stronger ability to scavenge DPPH, hydroxyl, and ABTS radicals, which was potentially associated with its high content of galacturonic acid and presence of the HG domain.</p></sec><sec><title>Conclusion</title><p>The results provide information for enhancing knowledge of the structureactivity relationship of pectic polysaccharides from <italic>V. peregrina</italic> and their potential application in the healthcare food field.</p></sec>