AUTHOR=Wang Zhen , Liu Hui , Fu Ranze , Ou Jinmei , Wang Bin 

TITLE=Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum

JOURNAL=Frontiers in Nutrition

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2023.1156798

DOI=10.3389/fnut.2023.1156798

ISSN=2296-861X

ABSTRACT=<sec><title>Introduction</title><p>This study aimed to investigate the structure characterization and antiinflammatory activity of a novel polysaccharide, PKP2-1, from the rhizomes of <italic>Polygonatum kingianum</italic> Coll. and Hemsl.</p></sec><sec><title>Methods</title><p>We isolated a novel polysaccharide, PKP2-1, from the rhizomes of <italic>Polygonatum kingianum</italic> Coll. and Hemsl. for the first time, which was then successively purified through hot-water extraction, 80% alcohol precipitation, anion exchange and gel permeation chromatography. The <italic>in vitro</italic> anti-inflammatory activity of PKP2-1 in MH7A cells was assessed using a CCK-8 kit assay.</p></sec><sec><title>Results</title><p>Monosaccharide composition assay revealed that PKP2-1 was mainly composed of glucose, galactose, mannose, and glucuronic acid at an approximate molar ratio of 6:2:2:1. It had a molecular weight of approximately 17.34 kDa. Structural investigation revealed that the backbone of PKP2-1 consisted of (→2, 3)-α-D-Galp(4→, →2)-α-D-Manp(3→, →2)-β-D-Glcp(4→) and α-D-Glcp(3→) residues with side chains (→2)-β-D-Glcp(4→, →1)-α-D-Galp(4→) and α-D-Glcp(3→) branches located at O-3 position of (→2, 3)-α-D-Galp(4→). The <italic>in vitro</italic> anti-inflammatory activity of PKP2-1 in MH7A cells revealed that PKP2-1 could reduce the expression of IL-11β and IL-6, increase the expression of IL-10 and induce apoptosis of synovial fibroblasts.</p></sec><sec><title>Conclusion</title><p>The PKP2-1 could inhibit MH7A cell growth and potentially be exploited as an anti-inflammatory agent.</p></sec>