AUTHOR=Miró Lluïsa , Rosell-Cardona Cristina , Amat Concepció , Polo Javier , Moretó Miquel , Pérez-Bosque Anna 

TITLE=Dietary supplementation with spray-dried animal plasma improves vaccine protection in aged mice

JOURNAL=Frontiers in Nutrition

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2023.1050961

DOI=10.3389/fnut.2023.1050961

ISSN=2296-861X

ABSTRACT=<sec><title>Background</title><p>Senescence is characterized by an aggravated inflammatory state that reduces vaccine responsiveness. Dietary supplementation with spray-dried porcine plasma (SDP) exerts anti-inflammatory effects in different mucosal areas. We aimed to determine if the anti-inflammatory properties of SDP improve the efficiency of immunization in senescent animals.</p></sec><sec><title>Methods</title><p>Experiments were performed in 2-month-old and 6-month-old male SAMP8 mice fed control or SDP (8%) feeds for 4  months. The mice received nasal doses of 2.5  μg of <italic>Staphylococcus aureus</italic> enterotoxin B (SEB) or vehicle every 15  days (i.e., 3 times). Fifteen days after the last dose, a lethal shock was induced by intraperitoneal administration of SEB and LPS.</p></sec><sec><title>Results</title><p>Immunization increased anti-SEB IgA in intestinal and bronchoalveolar fluid (<italic>p</italic> &lt; 0.05). After the lethal shock, all immunized aged mice that were supplemented with SDP survived, in contrast to only 66% of those fed the control feed (<italic>p</italic> &lt; 0.05). Moreover, after the lethal challenge, aged mice showed higher expression levels of pro-inflammatory cytokines (<italic>Il-6</italic>, <italic>Tnf-α</italic>, <italic>Ifn-γ</italic>, and <italic>Il-1β</italic>) in jejunal and (<italic>Tnf-α</italic>, and <italic>Il-1β</italic>) in lung tissues (<italic>p</italic> &lt; 0.05), which were reduced by SDP supplementation (<italic>p</italic> &lt; 0.05). Furthermore, in senescent mice, SDP supplementation augmented <italic>Il-4</italic> and <italic>Il-10</italic> expression in both tissues (<italic>p</italic> &lt; 0.05).</p></sec><sec><title>Conclusion</title><p>SDP reduces the mucosal inflammation associated with aging, improving vaccine protection in senescent mice.</p></sec>