AUTHOR=Ji Linbao , Zhang Lian , Liu Hu , Shen Jiakun , Zhang Yu , Lu Lin , Zhang Xiujun , Ma Xi 

TITLE=Bacillus subtilis M6 improves intestinal barrier, antioxidant capacity and gut microbial composition in AA broiler

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.965310

DOI=10.3389/fnut.2022.965310

ISSN=2296-861X

ABSTRACT=<p><italic>Bacillus subtilis</italic> can secret a variety of substances to improve human and animal gut health <italic>via</italic> inhibiting the proliferation of pathogenic bacteria. In this study, a fast-growing and stress-resistant strain of <italic>Bacillus subtilis M6</italic> (<italic>B. subtilis M6</italic>) were isolated, which showed a strong antibacterial activity to <italic>E. coli</italic> K88, <italic>S. typhimurium</italic> ATCC14028, and <italic>S. aureus</italic> ATCC25923 <italic>in vitro</italic>. <italic>In vivo</italic> studies showed that <italic>B. subtilis M6</italic> can significantly improve the average daily gain (ADG) using an AA broiler model. Dietary <italic>B. subtilis M6</italic> improved the intestinal morphology. The villus height of jejunum and ileum were significantly increased. The concentration of malondialdehyde (MDA) in the ileal mucosa was significantly reduced in <italic>B. subtilis M6</italic> treatment group, which suggested the oxidative stress of the ileum was significantly relieved. Though the β diversity of treatments was not significantly, <italic>B. subtilis M6</italic> improved the composition of intestinal microbes, especially at the level of caecum genus, the dominant genus was changed from <italic>Ruminococcus</italic> to <italic>Akkermansia</italic>, which indicated the change of intestinal carbohydrate nutrition. In conclusion, these data indicate that the <italic>B. subtilis</italic> M6 shows a probiotic potential to improve intestinal health <italic>via</italic> altering gut microbiota.</p>